FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295144
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGCAGGGTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGA9950.33712357357764344No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8650.2930772775323232No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8620.29206082454666193No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8500.287995012604017No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7690.26055078199116366No Hit
GTGCTGGTGAACGCACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7390.2503862521345513No Hit
CTCCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6870.23276773371642318No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG6470.21921502724094No Hit
CTCCATCTGCCTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCG6290.2131163093269726No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT6170.20905049738432763No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6020.20396823245602147No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG6000.2032905971322473No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAGG5970.20227414414658607No Hit
GTTGTGAGTAAGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG5950.2015965088228119No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA5920.2005800558371507No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA5870.19888596752771526No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA5830.19753069688016694No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5540.1877049846854417No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATCAAAGCAGGATATCCACAT5540.1877049846854417No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT5420.18363917274279673No Hit
ATCTCATCGAAGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATC5280.17889572547637764No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5270.17855690781449055No Hit
CAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAACA5090.17245818990052314No Hit
GACCTTTAACCTAACCTCCTCCCCTAACTCCTCCCACTCATTAAACACAC5010.16974764860542652No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT4950.16771474263410402No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT4910.1663594719865557No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC4760.16127720705824952No Hit
GGATAATACGGTTTAATCCCTTTATCTAAGGGCAAATATTTAGTAACATT4740.16059957173447537No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4710.15958311874881415No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC4700.15924430108692705No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4440.150435041877863No Hit
CCCTTTTTTCAAAGCCAACTCAGACAATCCAGATTGGGACCTCAACCCGC4400.14907977123031468No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCATGTTGCTGTACA4220.14298105331634728No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA4190.14196460033068603No Hit
CTATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGT4170.14128696500691187No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG4010.1358658824167186No Hit
CAGTAAACAGTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTC3980.13484942943105738No Hit
CCCTAAAAGACCCACAATTCGTTGACATACTTTCCAATCAATAGGCCTGT3950.13383297644539613No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT3880.1314612528121866No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA3850.13044479982652535No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA3770.12773425853142872No Hit
GCGTAAAAGGGGCGGCAAACCCTAAAAGACCCACAATTCGTTGACATACT3770.12773425853142872No Hit
AACGCGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGC3760.12739544086954163No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC3700.1253625348982192No Hit
CTTTTAGGGTTTGCCGCCCCTTTTACGCAATGTGGATATCCTGCTTTGAT3660.12400726425067087No Hit
GATCATTAACCTAACCTCCTCCCCTAACTCCTCCCACTCATTAAACACAC3660.12400726425067087No Hit
CTCAAACAATCCAGATTGGGACCTCAACCCGCACAAGGACAACTGGCCGG3580.12129672295557424No Hit
ACTACATACTCTGGATAATACGGTTTAATCCCTTTATCTAAGGGCAAATA3560.12061908763180008No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT3540.11994145230802593No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTT3510.11892499932236468No Hit
ATCTTAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG3500.1185861816604776No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT3470.11756972867481637No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC3440.11655327568915513No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC3430.11621445802726806No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA3370.11418155205594557No Hit
CATTTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCC3320.11248746374651018No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATATCCACATTGCGTAAAA3320.11248746374651018No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT3270.11079337543707479No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3250.11011574011330064No Hit
CCCCTAACTCCTCCCACTCATTAAACACACAGTCTTTGAAGTATGCCTCA3240.10977692245141356No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT3200.10842165180386523No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3180.10774401648009108No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT3150.10672756349442984No Hit
CTTTTACGCAATGTGGATATCCTGCTTTGATGCCTTTATATGCATGTATA3140.10638874583254276No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG3140.10638874583254276No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG3110.10537229284688153No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC3100.10503347518499445No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3100.10503347518499445No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3090.10469465752310737No Hit
CATTAACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCC3070.10401702219933322No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT3060.10367820453744614No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA3060.10367820453744614No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3030.10266175155178489No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3030.10266175155178489No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA3030.10266175155178489No Hit
TAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTGCAGT3020.10232293388989781No Hit
CAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTAT3010.10198411622801073No Hit
GACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGGATCGG3000.10164529856612366No Hit
CCAACCTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTCTGCG2970.10062884558046242No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2970.10062884558046242No Hit
CAATTCTCTAGGGGGAACACCCGTGTGTCTTGGCCAAAATTCGCAGTCCC2970.10062884558046242No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)