FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413524
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16460.3980421934398004No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16220.39223841905185675No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG15310.37023244116423715No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT14210.3436318085528289No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13960.33758621023205426No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG12630.30542362716553334No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCCACACTTCCTGA11470.2773720509571391No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG10160.24569311575628017No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT8920.2157069480852381No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8560.20700128650332264No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT8550.20675946257049166No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCACCAACTTACAAGGCC8490.20530851897350577No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG8340.201681159981041No Hit
GTTAGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACAC8260.19974656851839312No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA8000.19345914626478752No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7920.19152455480213965No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT7670.18547895648136503No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGATAGCACGACAGAAT7660.18523713254853408No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTCCCAATTACATATCCCATGAA7600.18378618895154816No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7440.1799170060262524No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7190.1738714077054778No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7110.17193681624282994No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6990.1690349290488581No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAAACTCTATG6990.1690349290488581No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6800.16444027432506939No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6470.15646008454164692No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT6380.15428366914616806No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAACGGCACTAGTAAACTGAG6270.15162360588502724No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC6030.1458198314970836No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5900.1426761203702808No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5870.14195064857178785No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5830.14098335284046393No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5820.14074152890763292No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5810.14049970497480196No Hit
CCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTC5780.139774233176309No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCG5780.139774233176309No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT5660.13687234598233716No Hit
CTTACCAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC5650.1366305220495062No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA5500.1330031630570414No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTAC5490.13276133912421045No Hit
ATGTATACTTGCGGGATAGCACGACAGAATTGTCAGTCCCGATAAGGTTT5400.1305849237287316No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA5360.12961762799740764No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5350.12937580406457666No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5280.12768303653475976No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5200.1257484450721119No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCTCTAACAGTAAATGAAACCAG5140.124297501475126No Hit
TGGTAAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACACGG5130.12405567754229502No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA5130.12405567754229502No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5090.12308838181097108No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT5060.12236291001247812No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5030.12163743821398515No Hit
CTCACCAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC5010.1211537903483232No Hit
CTCTAACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGA4990.12067014248266121No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4960.11994467068416827No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4870.11776825528868941No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC4840.11704278349019645No Hit
CTGTTAGAGGTCCCACAAATTGCTTACACCTATCAACAATGTCTTCCTGC4780.11559183989321056No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4780.11559183989321056No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4770.11535001596037957No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4760.11510819202754857No Hit
GTTTACAGGAAGTTTCCTAAAACATACTTTGATTTTTTGTACAATATGTT4760.11510819202754857No Hit
CTCACTAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC4740.11462454416188662No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4710.11389907236339365No Hit
ATCTTATCCACACTTCCTGAAACTACTGTTGTTAGACGACGAGGCAGGTC4700.11365724843056269No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC4690.11341542449773169No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4680.11317360056490071No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4670.11293177663206971No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTA4670.11293177663206971No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4670.11293177663206971No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG4650.11244812876640775No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA4610.11148083303508381No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG4600.11123900910225283No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4590.11099718516942184No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4590.11099718516942184No Hit
GGAACATATTGTACAAAAAATCAAAGTATGTTTTAGGAAACTTCCTGTAA4560.11027171337092889No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG4560.11027171337092889No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4530.10954624157243593No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4520.10930441763960495No Hit
CCCCTATCTTATCCACACTTCCTGAAACTACTGTTGTTAGACGACGAGGC4500.10882076977394299No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA4470.10809529797545005No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4450.10761165010978807No Hit
GTGCTGGGGTTGGAATTGAGATTGGGATCATCCTTACTAAAGGTTTGGCT4440.10736982617695708No Hit
CTATTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG4420.10688617831129511No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA4410.10664435437846413No Hit
GCTTACACCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAA4380.10591888257997117No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT4350.10519341078147823No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4350.10519341078147823No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC4310.10422611505015429No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAAT4290.10374246718449232No Hit
ATGTAAGACCTTGGGCAGGTTCTGGTGGGCGTTCACGGTGGTTTCCATGC4290.10374246718449232No Hit
CACTCACTAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTG4260.10301699538599937No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4250.10277517145316838No Hit
GGATTAAAGTTAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC4240.10253334752033738No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4160.1005987560576895No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4140.10011510819202755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)