FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences413524
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG14240.3443572803513218No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13190.31896576740406846No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT13110.3170311759414206No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC12870.31122740155347695No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC12760.3085673382923361No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG11710.28317582534508273No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCCACACTTCCTGA11200.27084280477070255No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG9890.23916386956984356No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT8340.201681159981041No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC8250.19950474458556214No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT7960.1924918505334636No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCACCAACTTACAAGGCC7940.1920082026678016No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG7900.19104090693647768No Hit
GTTAGTGAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACAC7870.19031543513798474No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA7770.1878971958096749No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7520.18185159748890029No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGATAGCACGACAGAAT7220.17459687950397076No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7090.17145316837716795No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTCCCAATTACATATCCCATGAA6990.1690349290488581No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAAACTCTATG6850.16564939398922432No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC6750.16323115466091448No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT6530.1579110281386328No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6450.15597643667598493No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6430.155492788810323No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6230.1506563101537033No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA6190.14968901442237936No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT6100.1475125990269005No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6080.14702895116123854No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5950.14388524003443573No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA5880.14219247250461883No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5870.14195064857178785No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC5800.14025788104197096No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT5540.13397045878836536No Hit
CTTACCAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC5540.13397045878836536No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG5350.12937580406457666No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAACGGCACTAGTAAACTGAG5270.12744121260192878No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5270.12744121260192878No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT5200.1257484450721119No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCG5170.12502297327361894No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5140.124297501475126No Hit
ATGTATACTTGCGGGATAGCACGACAGAATTGTCAGTCCCGATAAGGTTT5070.12260473394530909No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC5030.12163743821398515No Hit
GTTTACAGGAAGTTTCCTAAAACATACTTTGATTTTTTGTACAATATGTT5020.12139561428115418No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5010.1211537903483232No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA5010.1211537903483232No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5000.1209119664154922No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4900.11849372708718237No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4900.11849372708718237No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4880.11801007922152039No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4880.11801007922152039No Hit
CAAATCTTTCTGTCCCCAATCCACTGGGATTCTTCCCCGATCATCAGTTG4790.11583366382604153No Hit
CTCACCAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC4780.11559183989321056No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4770.11535001596037957No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA4750.11486636809471759No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTAC4720.11414089629622465No Hit
ATCTTATCCACACTTCCTGAAACTACTGTTGTTAGACGACGAGGCAGGTC4690.11341542449773169No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4640.11220630483357677No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA4630.11196448090074577No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG4620.11172265696791481No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4590.11099718516942184No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4590.11099718516942184No Hit
GGAACATATTGTACAAAAAATCAAAGTATGTTTTAGGAAACTTCCTGTAA4550.11002988943809792No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4530.10954624157243593No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC4530.10954624157243593No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT4520.10930441763960495No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCTCTAACAGTAAATGAAACCAG4470.10809529797545005No Hit
CTCACTAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC4470.10809529797545005No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG4440.10736982617695708No Hit
CTGTTAGAGGTCCCACAAATTGCTTACACCTATCAACAATGTCTTCCTGC4400.10640253044563314No Hit
GTGCTGGGGTTGGAATTGAGATTGGGATCATCCTTACTAAAGGTTTGGCT4380.10591888257997117No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTA4370.10567705864714019No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAAT4370.10567705864714019No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC4340.10495158684864724No Hit
GGTGTAAGCAATTTGTGGGACCTCTAACAGTAAATGAAACCAGGAGACTA4330.10470976291581625No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4290.10374246718449232No Hit
TGGTAAGTGACTGGAGATTTGGGACTGCGAATTTTGGCCAAGACACACGG4240.10253334752033738No Hit
CACTCACTAACTTGTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTG4240.10253334752033738No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG4230.10229152358750641No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4230.10229152358750641No Hit
CCCCTATCTTATCCACACTTCCTGAAACTACTGTTGTTAGACGACGAGGC4220.10204969965467543No Hit
CTCTAACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGA4220.10204969965467543No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA4190.10132422785618247No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT4160.1005987560576895No Hit
GCTTACACCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAA4140.10011510819202755No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG4140.10011510819202755No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)