FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488698

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488698
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344711
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA23070.6692562755467624No Hit
ATCTCAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTG21610.6269019555511719No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTG18900.548285375285384No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17010.49345683775684557No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCCACACTTCCTGA16220.47053908926608085No Hit
GGTTCAGATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGTGAGAAAGTA12570.3646532892771046No Hit
ATGTAAGACCTTGGCCAGGTTCTGGTGGGCGTTCACGGTGGTCTCCATGC11600.33651377530743143No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11570.33564348106094666No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG10900.3162069095561209No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTCCCAATTACATATCCCATGAA10860.3150465172274746No Hit
GGTTGAGATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGTGAGAAAGTA10030.29096837640806356No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG9770.2834258262718625No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9290.2695011183281067No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC9220.2674704317529757No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT9210.26718033367081406No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT8710.25267542956273514No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAAACTCTATG8360.24252199668707988No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7870.22830719066116253No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT7460.21641316929253782No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA7430.21554287504605305No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA6890.1998775786093278No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT6850.1987171862806815No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6800.19726669586987358No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6730.19523600929474255No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT6620.19204493039096518No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT6580.19088453806231886No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6580.19088453806231886No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA6410.18595287066557203No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA6360.18450238025476412No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG6050.1755093397077552No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC5910.1714479665574931No Hit
ATCTTATCCACACTTCCTGAAACTACTGTTGTTAGACGACGAGGCAGGTC5890.17086777039316994No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5880.17057767231100834No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCACCAACTTACAAGGCC5840.16941727998236203No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT5820.16883708381803889No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5820.16883708381803889No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5490.15926384710670677No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5390.156362866285091No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGATAGCACGACAGAAT5380.15607276820292942No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5380.15607276820292942No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5380.15607276820292942No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTAC5280.15317178738131362No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC5270.15288168929915205No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5150.14940051231321308No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGCCAGGTTCTGGTGGGCGTTC5110.14824011998456676No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5080.14736982573808205No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC5080.14736982573808205No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT5060.1467896295737589No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC5000.14504904108078942No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4990.14475894299862785No Hit
CCCCTATCTTATCCACACTTCCTGAAACTACTGTTGTTAGACGACGAGGC4990.14475894299862785No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG4930.14301835450565836No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT4910.14243815834133522No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA4880.14156786409485048No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4790.13895698135539628No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4700.13634609861594205No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4700.13634609861594205No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4700.13634609861594205No Hit
TCGACAAGGCATGGGGACAAATCTTTCTGTCCCCAATCCACTGGGATTCT4660.13518570628729573No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC4650.13489560820513416No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4630.134315412040811No Hit
ATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC4610.13373521587648785No Hit
GTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGC4600.13344511779432627No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT4580.1328649216300031No Hit
GTTTACAGGAAGTTTCCTAAAACATACTTTGATTTTTTGTACAATATGTT4580.1328649216300031No Hit
CCTCGACAAGGCATGGGGACAAATCTTTCTGTCCCCAATCCACTGGGATT4580.1328649216300031No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAAT4570.13257482354784153No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4560.13228472546567993No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT4550.13199462738351836No Hit
ATGTATACTTGCGGGATAGCACGACAGAATTGTCAGTCCCGATAAGGTTT4460.12938374464406416No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC4380.12706295998677153No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4380.12706295998677153No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA4380.12706295998677153No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4370.12677286190460993No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4250.123291684918671No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCG4240.12300158683650944No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATACTTTGATTTTTTGT4210.12213129259002468No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4210.12213129259002468No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4180.12126099834353994No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4180.12126099834353994No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC4180.12126099834353994No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCA4180.12126099834353994No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4160.12068080217921678No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4150.12039070409705521No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG4150.12039070409705521No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4140.12010060601489363No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4130.11981050793273207No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG4090.11865011560408575No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG4080.11836001752192415No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAAGAGTCCTCTTATGT4040.11719962519327785No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG4030.11690952711111625No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTACGCCCCGTGGTCGGTCGGTACGG4030.11690952711111625No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG4020.1166194290289547No Hit
GTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTA4020.1166194290289547No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3950.11458874245382364No Hit
GGAACATATTGTACAAAAAATCAAAGTATGTTTTAGGAAACTTCCTGTAA3950.11458874245382364No Hit
CTGTTAGAGGTCCCACAAATTGCTTACACCTATCAACAATGTCTTCCTGC3940.11429864437166207No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCTCTAACAGTAAATGAAACCAG3930.11400854628950048No Hit
CTCTTATGTAAGACCTTGGCCAGGTTCTGGTGGGCGTTCACGGTGGTCTC3920.11371844820733891No Hit
GTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC3920.11371844820733891No Hit
GGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAGATCCCA3910.11342835012517732No Hit
AGATAGGGGCATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACT3850.11168776163220785No Hit
ATGTAATTGGGAGTTGGGGTTCCTTGCCACAGGAACATATTGTACAAAAA3840.11139766355004627No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3820.11081746738572311No Hit
GGATTAAAGTTAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC3810.11052736930356154No Hit
CCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAG3800.11023727122139995No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCA3790.10994717313923838No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3690.1070461923176226No Hit
CCTTTTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCC3680.10675609423546101No Hit
ATTTACATACTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACA3670.10646599615329942No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT3670.10646599615329942No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3660.10617589807113785No Hit
GCTTACACCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAA3650.10588579998897626No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG3620.10501550574249154No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3610.10472540766032995No Hit
GGTGTAAGCAATTTGTGGGACCTCTAACAGTAAATGAAACCAGGAGACTA3600.10443530957816838No Hit
GTCCCGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC3580.10385511341384522No Hit
GTTCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGTGAAGC3560.10327491724952206No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG3550.10298481916736048No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTA3540.10269472108519892No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAAGGGGCAGCG3530.10240462300303732No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA3530.10240462300303732No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT3530.10240462300303732No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3520.10211452492087576No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT3490.101244230674391No Hit
GCACCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGG3450.1000838383457447No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)