FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488699

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488699
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences344711
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCTCAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTG22460.6515602925349061No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTG18920.5488655714497072No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA18130.5259478229589425No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC14850.4307956520099445No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCCACACTTCCTGA14270.41396996324457297No Hit
GGTTCAGATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGTGAGAAAGTA12290.3565305429765804No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12060.34985828708686406No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG10540.3057633785983041No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTCCCAATTACATATCCCATGAA10170.29502974955832567No Hit
GGTTGAGATATTGTTTACTTAGAAAGGCCTTGTAAGTTGGTGAGAAAGTA9160.2657298432600062No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8880.257607096959482No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT8370.24281209476924148No Hit
ATGTAAGACCTTGGCCAGGTTCTGGTGGGCGTTCACGGTGGTCTCCATGC7940.23033787723629356No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAAACTCTATG7920.22975768107197045No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT7910.22946758298980885No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7750.2248260136752236No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA7620.22105473860712307No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC7380.2140923846352452No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA7270.21090130573146781No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGCAAGTATTTGGTAACATT7090.20567954025255938No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT7030.20393895175958993No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT6860.1990072843628431No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT6830.19813699011635835No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6570.1905944399801573No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA6430.18653306682989518No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCACCAACTTACAAGGCC6410.18595287066557203No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6400.18566277258341043No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6260.18160139943314835No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT6140.1781202224472094No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA6050.1755093397077552No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG5920.17173806463965466No Hit
ATCTTATCCACACTTCCTGAAACTACTGTTGTTAGACGACGAGGCAGGTC5560.16129453368183783No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5520.1601341413531915No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAGTC5490.15926384710670677No Hit
GCCTAGCAGCCATGGAAACGATGTATACTTGCGGGATAGCACGACAGAAT5460.15839355286022205No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC5460.15839355286022205No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5340.1549123758742831No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG5330.1546222777921215No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5320.15433217970995994No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC5320.15433217970995994No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA5280.15317178738131362No Hit
AGTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5210.15114110080618257No Hit
CTATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGT5160.14969061039537468No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA5120.14853021806672836No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5120.14853021806672836No Hit
ATCTAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA5030.14591933532727414No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4870.1412777660126889No Hit
TCTATGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4860.1409876679305273No Hit
GTATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC4860.1409876679305273No Hit
GGAACATATTGTACAAAAAATCAAAGTATGTTTTAGGAAACTTCCTGTAA4840.14040747176620416No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC4820.139827275601881No Hit
CAAATCTTTCTGTCCCCAATCCACTGGGATTCTTCCCCGATCATCAGTTG4800.13924707943755785No Hit
CCTCGACAAGGCATGGGGACAAATCTTTCTGTCCCCAATCCACTGGGATT4790.13895698135539628No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4780.13866688327323468No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA4750.13779658902674993No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG4700.13634609861594205No Hit
CCCCTATCTTATCCACACTTCCTGAAACTACTGTTGTTAGACGACGAGGC4700.13634609861594205No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG4690.13605600053378047No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA4680.1357659024516189No Hit
GTTTACTAGTGCCGTTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC4620.13402531395864942No Hit
ATGTATACTTGCGGGATAGCACGACAGAATTGTCAGTCCCGATAAGGTTT4610.13373521587648785No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA4600.13344511779432627No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC4520.13112433313703362No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACATACTTTGATTTTTTGT4500.13054413697271047No Hit
GAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGACCCTG4490.1302540388905489No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTAC4490.1302540388905489No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT4470.12967384272622576No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC4410.12793325423325624No Hit
TCGACAAGGCATGGGGACAAATCTTTCTGTCCCCAATCCACTGGGATTCT4400.12764315615109467No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4390.1273530580689331No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG4360.12648276382244839No Hit
GTTTACAGGAAGTTTCCTAAAACATACTTTGATTTTTTGTACAATATGTT4330.12561246957596361No Hit
GGATATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAAT4330.12561246957596361No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA4310.12503227341164047No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT4290.12445207724731731No Hit
ATTTACATACTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACA4290.12445207724731731No Hit
ATGTTAATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCAC4270.12387188108299416No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4250.123291684918671No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4240.12300158683650944No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC4200.1218411945078631No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA4170.12097090026137837No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC4090.11865011560408575No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4080.11836001752192415No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA4030.11690952711111625No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCTCTAACAGTAAATGAAACCAG4020.1166194290289547No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4010.1163293309467931No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA3990.11574913478246994No Hit
ATGTAATTGGGAGTTGGGGTTCCTTGCCACAGGAACATATTGTACAAAAA3970.11516893861814678No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT3950.11458874245382364No Hit
GCTTACACCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAA3940.11429864437166207No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3940.11429864437166207No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG3890.11284815396085417No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCG3860.11197785971436942No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT3830.1111075654678847No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA3830.1111075654678847No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG3820.11081746738572311No Hit
GTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTC3790.10994717313923838No Hit
CCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCA3760.10907687889275364No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGCCAGGTTCTGGTGGGCGTTC3750.10878678081059207No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGACCTTTAACCTAATCTCCTCC3730.10820658464626891No Hit
AGATAGGGGCATTTGGTGGTCTATATGCAGGAGGAGTGCGAATCCACACT3690.1070461923176226No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG3690.1070461923176226No Hit
ACCAAATACTTGCCCTTAGATAAAGGAATCAAACCCTATTATCCCGAGCA3690.1070461923176226No Hit
TCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCACT3670.10646599615329942No Hit
CTGTTAGAGGTCCCACAAATTGCTTACACCTATCAACAATGTCTTCCTGC3650.10588579998897626No Hit
GGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAGATCCCA3650.10588579998897626No Hit
CCTTTTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCC3640.10559570190681469No Hit
GTAATTGGGAGTTGGGGTTCCTTGCCACAGGAACATATTGTACAAAAAAT3610.10472540766032995No Hit
GGATTAAAGTTAGGTACCGTAGAAGAATAAAGCCCCGTAAAGTTTCCCAC3610.10472540766032995No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACCTTT3610.10472540766032995No Hit
TTTTACGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTT3600.10443530957816838No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG3570.10356501533168364No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT3560.10327491724952206No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTACGCCCCGTGGTCGGTCGGTACGG3540.10269472108519892No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG3540.10269472108519892No Hit
TCCTGAAACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAA3530.10240462300303732No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3530.10240462300303732No Hit
GTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGGCAGTA3520.10211452492087576No Hit
ATTGTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA3470.10066403451006786No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)