FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488700

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488700
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175623
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8710.4959487083126925No Hit
CTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACAT6600.37580499137356727No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6440.3666945673402687No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA6280.35758414330697No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5540.31544843215296403No Hit
GTACAAAAGCTTAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT4620.2630634939614971No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4340.2471202519032245No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4280.24370384289073757No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4170.23744042636784476No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA4110.23402401735535777No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT3950.22491359332205918No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG3830.21808077529708522No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3800.21637257079084177No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT3760.2140949647825171No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3740.21295616177835477No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAGGGCATTAAACCATAT3640.20726214675754312No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT3500.1992905257284069No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA3450.19644351821800107No Hit
CTTTTGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACAT3390.19302710920551408No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA3350.19074950319718942No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC3280.1867636926826213No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC3250.1850554881763778No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC3040.1730980566326734No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC2920.16626523860769946No Hit
ACATCAAACCTCGACAAGGCATGGGGACAAATCTTTCGGTTCCCAATCCT2860.16284882959521246No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG2790.15886301908064432No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA2720.1548772085660762No Hit
GGAAAGTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGT2710.15430780706399502No Hit
ACTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT2640.1503219965494269No Hit
GTTGTCAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2580.14690558753693994No Hit
CTCTTACAGTCAATGAAAAAAGGAGGTTGAAATTAATTATGCCTGCTAGG2540.14462798152861528No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTCAACCTCCTTTTTTCA2540.14462798152861528No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC2540.14462798152861528No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG2510.14291977702237177No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC2460.14007276951196598No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2410.13722576200156017No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG2370.1349481559932355No Hit
CTCCAAAGGATACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA2360.13437875449115436No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG2350.13380935298907318No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC2340.13323995148699203No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2340.13323995148699203No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT2290.1303929439765862No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT2220.1264071334620181No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC2210.12583773195993692No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGTACAACAGAGT2150.12242132294744994No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT2140.12185192144536879No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA2140.12185192144536879No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2120.12071311844120645No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC2120.12071311844120645No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT2110.12014371693912529No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT2090.11900491393496296No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2060.11729670942871948No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG2030.11558850492247598No Hit
TTCGAAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAGAGA2020.11501910342039483No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2020.11501910342039483No Hit
GTATGGATCGGCAGAGGAGCCACGAAGGTTCCACGCATGCGGCGATGGCC2010.11444970191831366No Hit
CCTAGTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA2000.11388030041623251No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG1980.11274149741207018No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC1950.11103329290582668No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAAGCAGGATAACCACAT1930.10989448990166437No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG1910.10875568689750204No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG1910.10875568689750204No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC1900.10818628539542088No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG1880.10704748239125855No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA1830.10420047488085274No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATA1820.10363107337877156No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC1820.10363107337877156No Hit
GTACTAAAGCTTAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT1810.10306167187669041No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA1800.10249227037460924No Hit
GTACTAAAGATTAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT1800.10249227037460924No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA1800.10249227037460924No Hit
CCCCAAAAGACCGCCGTGTGGTGGGGTGAACCCTGGCCCGAATGCTCCCG1800.10249227037460924No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT1780.10135346737044691No Hit
GGATAATATGGTTTAATGCCCTTATCCAAGGGCAAATATTTGGTAAGGTT1770.10078406586836576No Hit
GTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGTTGTGG1760.1002146643662846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)