FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488701

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488701
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175623
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8700.4953793068106113No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6110.3479043177715903No Hit
CTTTAGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACAT5960.33936329524037284No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5220.2972275840863668No Hit
GTACAAAAGCTTAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT4730.26932691048438984No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA4220.24028743387825058No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA4210.23971803237616943No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT4020.22889940383662732No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3990.22719119933038381No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATAACCACATTGTGTAAAA3970.2260523963262215No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT3780.2152337677866794No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3740.21295616177835477No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT3700.2106785557700301No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG3660.20840094976170548No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA3620.20612334375338082No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3320.18904129869094594No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAGGGCATTAAACCATAT3220.18334728367013434No Hit
CCTTTGGAGTGTGGATTCGCACTCCTCCTGCTTACAGACCACCAAATGCC3080.17537566264099805No Hit
CTTTTGTACAATATGTTCTTGCGGTAAAGTACCCCAACTTCCAATTACAT3010.1713898521264299No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG2880.1639876325993748No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTCAACCTCCTTTTTTCA2750.15658541307231968No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAA2750.15658541307231968No Hit
GACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC2730.15544661006815735No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2710.15430780706399502No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC2680.15259960255775154No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG2660.15146079955358924No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA2600.14804439054110224No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC2570.14633618603485876No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAGTCTTTGA2560.1457667845327776No Hit
ACATCAAACCTCGACAAGGCATGGGGACAAATCTTTCGGTTCCCAATCCT2520.14348917852445295No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT2490.14178097401820947No Hit
CTCTTACAGTCAATGAAAAAAGGAGGTTGAAATTAATTATGCCTGCTAGG2460.14007276951196598No Hit
CTCCAAAGGATACCAAATACTCAAGGACAGTTTCTCTTCCAAAAGTAAGA2450.1395033680098848No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC2360.13437875449115436No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG2350.13380935298907318No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC2310.13153174698074854No Hit
GTTGTCAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2300.1309623454786674No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC2280.12982354247450506No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2280.12982354247450506No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA2270.12925414097242388No Hit
GGAAAGTATAGGCCCCTCACTCTGGGGTCTAGCAGAGCTTGGTGGAATGT2250.12811533796826155No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2160.1229907244495311No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2150.12242132294744994No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG2140.12185192144536879No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT2120.12071311844120645No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG2120.12071311844120645No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT2110.12014371693912529No Hit
ACTGACAACAGTGCCAGTAGCACCTCCTCCTGCCTCCACCAATCGGCAGT2090.11900491393496296No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCTTTATATGCATGTATA2070.11786611093080063No Hit
GTACTAAAGATTAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT2060.11729670942871948No Hit
GGATAATATGGTTTAATGCCCTTATCCAAGGGCAAATATTTGGTAAGGTT2020.11501910342039483No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC2010.11444970191831366No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG2000.11388030041623251No Hit
ATATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT1990.11331089891415133No Hit
GTATGGATCGGCAGAGGAGCCACGAAGGTTCCACGCATGCGGCGATGGCC1990.11331089891415133No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC1980.11274149741207018No Hit
CTATTAATAATATCCTCCTGTAAATGAATGTGAGGAAAGGAGGGAGTTTG1960.11160269440790786No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT1960.11160269440790786No Hit
CCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGTACAACAGAGT1950.11103329290582668No Hit
TTCGAAAACTGCCTGTAAATAGACCTATTGATTGGAAAGTATGTCAGAGA1940.11046389140374553No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA1910.10875568689750204No Hit
GCTTAGATTGTATACATGCATATAAAGGCATTAAAGCAGGATAACCACAT1860.10590867938709624No Hit
GTACTAAAGCTTAAGCAATGTTTTCGAAAACTGCCTGTAAATAGACCTAT1810.10306167187669041No Hit
GGGTTCACCCCACCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA1800.10249227037460924No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT1790.10192286887252809No Hit
CTTACAGTCAATGAAAAAAGGAGGTTGAAATTAATTATGCCTGCTAGGTT1760.1002146643662846No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)