FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488702

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488702
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528382
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAGAGTCCAA19890.37643220245958414No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16230.3071641350386652No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG15820.2994045974314038No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15490.2931591159426324No Hit
GTGTAAAAGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT15040.284642550276126No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA14810.28028963893546716No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14250.2696912461060369No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACA14040.2657168487950006No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13260.2509548016397228No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT12860.2433845210472726No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC11690.2212414503143559No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG11520.2180240810625646No Hit
GGAAAGTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT11300.21386042673671699No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG10680.20212649181841924No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA10650.20155872077398548No Hit
CATCTAAACCCTAATAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT10240.19379918316672406No Hit
GGATATTATCAATAGATGTCAACAGTATGTGGGCCCTCTTACAGTTAACG10030.18982478585568774No Hit
CCCTTAACTTCATGGGATACGTAATTGGAAGTTGGGGTACCTTGCCACAG9820.1858503885446514No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9800.1854718745150289No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9710.18376856138172762No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA9620.18206524824842635No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACAC9620.18206524824842635No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9620.18206524824842635No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9370.17733382287814498No Hit
ATATAAGAGAGAAACCACACGCAGCGCCTCATTTTGTGGGTCACCATATT9070.17165611243380735No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACGTATCCCATGAA9020.1707098273597511No Hit
GCACTAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTGGCCAC8700.16465360288579095No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT8620.16313954676730094No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8470.16030069154513213No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAGGTTCAATCTCCTTTTTTCG8440.15973292050069837No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8420.15935440647107585No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT8400.15897589244145333No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG8350.15802960736739707No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTCAC8160.15443372408598324No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGCACGACAGAGT8080.15291966796749323No Hit
GCTTACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCC8070.15273041095268197No Hit
GTATAAAGAATTTGGAGCTTCCGTGGAGTTACTCTCTTTTTTGCCTTCTG7840.14837749961202312No Hit
CTGTAAGAGGGCCCACATACTGTTGACATCTATTGATAATATCCTCTTTC7800.1476204715527781No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC7750.14667418647872182No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7660.14497087334542055No Hit
CCCCAAAAGACTGCCGTGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCA7540.1426997891676855No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG7450.14099647603438423No Hit
GTCATTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG7400.14005019096032795No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7320.1385361348418379No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7320.1385361348418379No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7280.13777910678259292No Hit
GGAACATATTGTACTAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA7240.13702207872334787No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC7140.13512950857523534No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT7120.13475099454561282No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG7110.13456173753080156No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC7090.13418322350117906No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7080.13399396648636783No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7040.1332369384271228No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT7040.1332369384271228No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTA7040.1332369384271228No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6900.13058734021976526No Hit
CCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTTTGAATTGG6890.130398083204954No Hit
ATATAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT6810.12888402708646396No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTAGTACAATATGTT6790.12850551305684146No Hit
ATCCTAACCAGACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6720.12718071395316266No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6710.1269914569383514No Hit
GGGTAAAGGTTCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6700.12680219992354017No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC6660.12604517186429515No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACACGCAGCGCCTCAT6610.1250988867902389No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6540.12377408768656009No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6530.12358483067174886No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6520.1233955736569376No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCAGCGGACGACCCGTCTCG6520.1233955736569376No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC6510.12320631664212633No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6450.12207077455325882No Hit
GTCTACGTCCCGTCGGCGCTGAATCCAGCGGACGACCCGTCTCGGGGCCG6450.12207077455325882No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGATCTTCC6410.1213137464940138No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6400.12112448947920254No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6320.11961043336071252No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACACGCAGCGCC6300.11923191933109001No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6250.11828563425703374No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT6200.11733934918297746No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTGG6190.11715009216816621No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6100.11544677903486493No Hit
ATCCTAACCTGACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6090.11525752202005367No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6080.1150682650052424No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAGTGTTTGCTG5990.11336495187194114No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5920.11204015276826236No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAATGACCGACCT5910.1118508957534511No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTGCCAGC5900.11166163873863985No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5860.11090461067939483No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5840.11052609664977232No Hit
CTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5770.10920129754609353No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5740.10863352650165978No Hit
CTCCCAGTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAG5730.10844426948684854No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5710.10806575545722602No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC5680.10749798441279225No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5680.10749798441279225No Hit
TCACCATACAGCACTAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGA5660.10711947038316975No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACACGC5640.10674095635354724No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5630.10655169933873598No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTGACAC5600.10598392829430223No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAATC5580.10560541426467972No Hit
GTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTC5550.10503764322024596No Hit
TTATATAAGAGAGAAACCACACGCAGCGCCTCATTTTGTGGGTCACCATA5550.10503764322024596No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG5540.1048483862054347No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5540.1048483862054347No Hit
GGAAGGTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT5520.10446987217581219No Hit
CCATACAGCACTAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATC5500.1040913581461897No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5470.10352358710175594No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTGAACCTAATTATGCCTGCTAGG5470.10352358710175594No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5440.10295581605732217No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5430.1027665590425109No Hit
GTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCTG5420.10257730202769967No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5410.10238804501288841No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5370.10163101695364338No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAGTG5350.10125250292402088No Hit
GTATGCATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACA5350.10125250292402088No Hit
TATCAATAGATGTCAACAGTATGTGGGCCCTCTTACAGTTAACGAAAAAA5330.10087398889439837No Hit
CTCCTAGTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAG5300.10030621784996462No Hit
GTCCAACTGGTGACCGGGAAAGAATCCCAGAGGATTGGGAACCGAAAGAT5290.10011696083515335No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)