FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488703

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488703
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences528382
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG17600.33309234606780697No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCATTGACATTGCTGAGAGTCCAA17320.32779314965309186No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG16520.31265258846819155No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15110.2859673493798048No Hit
GTGTAAAAGGAGCGGCAAAGCCCAGAAGACCCACAATTCTTTGACATACT14690.2780185547577321No Hit
ATACCTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA14360.2717730732689607No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13970.2643920496913218No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACA13480.25511845596557037No Hit
CCTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT13010.24622337626944144No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT11760.22256624941803466No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG11140.2108323144997369No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG11090.20988602942568066No Hit
GGAAAGTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT10300.1949347252555916No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA10090.19096032794455525No Hit
ATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA9960.18849998675200896No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC9830.18603964555946265No Hit
CCCTTAACTTCATGGGATACGTAATTGGAAGTTGGGGTACCTTGCCACAG9820.1858503885446514No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC9770.18490410347059513No Hit
CATCTAAACCCTAATAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT9640.18244376227804884No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9590.18149747720399256No Hit
GGATATTATCAATAGATGTCAACAGTATGTGGGCCCTCTTACAGTTAACG9470.1792263930262575No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9340.17676605183371122No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACAC8920.16881725721163854No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAGGTTCAATCTCCTTTTTTCG8700.16465360288579095No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8630.16332880378211218No Hit
CTACTGTACCTGTCTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTCAC8550.16181474766362214No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA8470.16030069154513213No Hit
ATATAAGAGAGAAACCACACGCAGCGCCTCATTTTGTGGGTCACCATATT8230.15575852318966202No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACGTATCCCATGAA8210.15538000916003952No Hit
GCACTAAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7970.1508378408045694No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7880.14913452767126814No Hit
GTATAAAGAATTTGGAGCTTCCGTGGAGTTACTCTCTTTTTTGCCTTCTG7870.14894527065645688No Hit
GCTTACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCC7820.14799898558240063No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7810.14780972856758937No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC7750.14667418647872182No Hit
CCCCAAAAGACTGCCGTGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCA7740.14648492946391056No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT7700.14572790140466557No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC7670.14516013036023181No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTAGTACAATATGTT7380.13967167693070542No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG7330.13872539185664917No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTACTTCCGAGAGAGCACGACAGAGT7260.1374005927529704No Hit
CTGTAAGAGGGCCCACATACTGTTGACATCTATTGATAATATCCTCTTTC7210.13645430767891412No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7170.1356972796196691No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7150.1353187655900466No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG7140.13512950857523534No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTGTA7070.13380470947155657No Hit
GGGTAAAGGTTCAGATATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA6990.13229065335306653No Hit
GGAACATATTGTACTAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA6930.13115511126419901No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6920.13096585424938775No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC6840.12945179813089772No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT6800.1286947700716527No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6790.12850551305684146No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCAGCGGACGACCCGTCTCG6790.12850551305684146No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG6750.12774848499759645No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6720.12718071395316266No Hit
GTTTCCCACCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGAT6710.1269914569383514No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6710.1269914569383514No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6660.12604517186429515No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA6620.12528814380505016No Hit
TCCGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6540.12377408768656009No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT6500.12301705962731509No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTGG6500.12301705962731509No Hit
GTCTACGTCCCGTCGGCGCTGAATCCAGCGGACGACCCGTCTCGGGGCCG6450.12207077455325882No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAGTGTTTGCTG6440.12188151753844756No Hit
ATCCTAACCAGACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6410.1213137464940138No Hit
ATCCTAACCTGACTAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT6340.11998894739033501No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT6330.11979969037552378No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG6290.11904266231627875No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6280.11885340530146749No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6200.11733934918297746No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC6180.11696083515335495No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6120.11582529306448744No Hit
ATATAGAGCAGAGGCGGTGTCGAGGAGATCTCGAATAGAAGGAAAGAAGT6110.11563603604967618No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6090.11525752202005367No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6080.1150682650052424No Hit
CCATTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGATTGTTTGAATTGG6030.11412197993118614No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5990.11336495187194114No Hit
CTTTAATCCTGATTGGCAAACTCCCTCTTTTCCTCACATTCATTTGAAAG5970.11298643784231861No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTGCCAGC5920.11204015276826236No Hit
GTCATTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5880.11128312470901733No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGATCTTCC5880.11128312470901733No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAATGACCGACCT5790.10957981157571606No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACACGCAGCGCC5770.10920129754609353No Hit
CTCCCAGTACAAAGATCATTAACCTAGTCTCCTCCCCCAACTCCTCCCAG5710.10806575545722602No Hit
CTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC5690.10768724142760351No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5690.10768724142760351No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5660.10711947038316975No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5640.10674095635354724No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5590.10579467127949098No Hit
GCCTTCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAAC5580.10560541426467972No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5580.10560541426467972No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACCACACGCAGCGCCTCAT5580.10560541426467972No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5560.10522690023505722No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5530.10465912919062345No Hit
GTCCAACTGGTGACCGGGAAAGAATCCCAGAGGATTGGGAACCGAAAGAT5500.1040913581461897No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5500.1040913581461897No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5480.10371284411656717No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5460.10333433008694468No Hit
GTATACATCTAAACCCTAATAAAACTAAACGTTGGGGCTACTCCCTTAAC5390.1020095309832659No Hit
GGAAGGTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT5390.1020095309832659No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5370.10163101695364338No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAGTG5350.10125250292402088No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTGACAC5350.10125250292402088No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)