FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488704

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488704
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522649
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC28590.547021040889775No Hit
TCCATAGAGTGTGTAAATAATGTCTTGTTTGGAAGTAATGATTAACTAGA24810.47469716769763265No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC18770.35913203698849516No Hit
CTCTATGGAAGGCAGGTGTATTATATAAGAGAGAAACAACACATAGCGCC16490.31550811347577434No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT15700.3003928066446124No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT15530.29714014568094455No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA15420.2950354827044537No Hit
CCTCCAGCTTATAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA14780.28279017084123376No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG14510.2776241798989379No Hit
GTTTAGACTGAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACAT13920.2663355330250321No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC13140.2514115591917329No Hit
CAGTCAATGAGAAAAGAAGGCTGCAATTGATTATGCCTGCTAGGTTTTAT13140.2514115591917329No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12670.2424189082921808No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC12490.2389749143306502No Hit
CATTAACTGTGAGTGGGCCTACAAATTGTTCACATTTTTTGATAATGTCT12390.2370615843520221No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT12390.2370615843520221No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG11800.22577293747811628No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC11720.2242422734952138No Hit
ATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTTGTTTGGA11560.22118094552940884No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG11060.21161429563626832No Hit
CTCTTATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTTGT11020.2108489636448171No Hit
CCCTTATCCAATGGCAAATATTTGGTGACCTTTGGATAAAACCTAGCAGG10910.20874430066832617No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT10790.20644830469397243No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA10570.20223897874099062No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT10480.2005169817602253No Hit
TACCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10440.19975164976877408No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT10370.19841231878373441No Hit
GTATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACC10060.1924809958499873No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT9970.19075899886922199No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT9780.18712367190982857No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9720.1859756739226517No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9640.18444500993974924No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC9340.17870502000386493No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA9280.17755702201668805No Hit
GAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA9110.1743043610530203No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG9050.17315636306584342No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC8870.16971236910431284No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCTTCAACTACC8860.16952103610645003No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC8800.16837303811927318No Hit
CTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCT8740.1672250401320963No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA8740.1672250401320963No Hit
GTATGGATCGGCATAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC8490.16244171518552603No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8370.16014571921117232No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG8290.15861505522826982No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA8190.1567017252496417No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATT8190.1567017252496417No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8120.15536239426460205No Hit
CCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT7840.15000507032444335No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT7770.1486657393394037No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG7680.14694374235863839No Hit
TCCTAATACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGT7650.14636974336505No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC7640.14617841036718715No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT7570.1448390793821475No Hit
CTTCTAGATACCGCCTCAGCTCTGTTTCGGGAAGCCTTAGAGTCTCCTGA7560.14464774638428468No Hit
TCACCATACTGCTCTCAGGCAAGCAATTCTGTGCTGGGGGGAACTAATGA7560.14464774638428468No Hit
GTTTTGACTGAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACAT7460.14273441640565657No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTCTGATGTGAT7430.14216041741206814No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT7400.14158641841847971No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7320.14005575443557722No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7170.13718575946763506No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTGACCTTTGGATAAAAC7160.13699442646977225No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG7150.13680309347190944No Hit
GCATAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAG6990.13374176550610448No Hit
CAGTTAATGAGAAAAGAAGGCTGCAATTGATTATGCCTGCTAGGTTTTAT6980.13355043250824167No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6700.128193108568083No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTATGGACTTCTTTCCTTCAGTACG6680.12781044257235735No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT6640.12704511058090612No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6600.12627977858945488No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6480.12398378261510115No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6470.12379244961723834No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA6310.12073112165143338No Hit
GCCTGAGGATGAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT6290.12034845565570775No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6280.12015712265784494No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA6260.11977445666211933No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6260.11977445666211933No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6250.1195831236642565No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6220.11900912467066808No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC6160.11786112668349122No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT6140.11747846068776559No Hit
CCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTC6130.11728712768990278No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA6070.11613912970272593No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA6030.11537379771127468No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA6020.11518246471341187No Hit
CTCTACTTCCAGGATCTTCAACTACCAGCACGGGACCATGCAGAACCTGC5980.11441713272196062No Hit
CATGTATTCAGTCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5950.11384313372837218No Hit
GTCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5950.11384313372837218No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5920.11326913473478377No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5920.11326913473478377No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCTCTCAGGCA5900.11288646873905814No Hit
GTGTAAATAATGTCTTGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT5870.11231246974546971No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA5850.11192980374974408No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGG5840.11173847075188127No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC5830.11154713775401848No Hit
CTATGGAAGGCAGGTGTATTATATAAGAGAGAAACAACACATAGCGCCTC5800.11097313876043002No Hit
ATTTATGCCTACAGCCTCCTAATACAAAGACCTTTAACCTCATCTCCTCC5800.11097313876043002No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5730.10963380777539036No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5720.10944247477752755No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5700.10905980878180192No Hit
TCTAGATACCGCCTCAGCTCTGTTTCGGGAAGCCTTAGAGTCTCCTGAGC5690.10886847578393911No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG5680.10867714278607632No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG5680.10867714278607632No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5620.10752914479889945No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACCGCATCTTGAGTCCCTTTTTACC5610.10733781180103664No Hit
AGTCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGT5560.1063811468117226No Hit
CATTGACTGTGAGTGGGCCTACAAATTGTTCACATTTTTTGATAATGTCT5550.10618981381385977No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT5520.10561581482027135No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5510.10542448182240854No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT5500.10523314882454572No Hit
ACCTCATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG5450.10427648383523166No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5430.10389381783950605No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT5380.102937152850192No Hit
GTTTTGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTC5370.1027458198523292No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAAGTAGAGGACAAACGGGCAAC5360.10255448685446639No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5340.10217182085874076No Hit
ATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACGAGCAG5340.10217182085874076No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5320.10178915486301514No Hit
ATATGGGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG5270.1008324898737011No Hit
GGGAAATACAGGCCTCTCACTCTGGGATCTTGCAGAGTTTGGTGGAAGGT5250.10044982387797546No Hit
CTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTC5230.10006715788224985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)