FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488705

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488705
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences522649
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTTGTTTGGAAGTAATGATTAACTAGA24900.4764191646783979No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC18800.3597060359820836No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG18780.359323369986358No Hit
CTCTATGGAAGGCAGGTGTATTATATAAGAGAGAAACAACACATAGCGCC17000.3252660963667777No Hit
CCTCCAGCTTATAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA15160.2900608247600206No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT14230.2722668559587792No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT14220.2720755229609164No Hit
GTTATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC13550.25925621210410815No Hit
CAGTCAATGAGAAAAGAAGGCTGCAATTGATTATGCCTGCTAGGTTTTAT12970.2481588982280651No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG12340.23610491936270805No Hit
CATTAACTGTGAGTGGGCCTACAAATTGTTCACATTTTTTGATAATGTCT12230.23400025638621713No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT12190.2332349243947659No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA12060.23074759542254936No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC12030.2301735964289609No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT11990.2294082644375097No Hit
CCCTTATCCAATGGCAAATATTTGGTGACCTTTGGATAAAACCTAGCAGG11950.22864293244605843No Hit
GTTTAGACTGAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACAT11900.22768626745674436No Hit
CTCTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA11660.22309427550803695No Hit
CTCTTATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTTGT11250.2152496225956617No Hit
ATGTAAGACCTTGGGCAAGAATTGATGGGCGTTCACGGTGGTCTCCATGC11110.21257096062558237No Hit
AATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG10950.20950963265977737No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT10510.20109098075381374No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT10410.19917765077518565No Hit
ATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTTGTTTGGA10340.197838319790146No Hit
CTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCT9880.18903700188845668No Hit
GTATTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACC9810.18769767090341702No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC9400.1798530179910418No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC9350.17889635300172774No Hit
GAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACATTGTGTAAAA9150.17506969304447154No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9060.17334769606370623No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAAAGCCCTTGTATGCATGTATT9040.1729650300679806No Hit
GTATGGATCGGCATAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCC8940.1710517000893525No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA8940.1710517000893525No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8780.16799037212354753No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT8740.1672250401320963No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT8720.16684237413637068No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGATGGGCGTTC8700.16645970814064506No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA8600.16454637816201698No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8360.1599543862133095No Hit
CAATAGGCCTGTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8170.15631905925391612No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGATCTTCAACTACC8160.1561277262560533No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7940.15191840030307147No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG7920.15153573430734585No Hit
TACCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7830.14981373732658054No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7730.14790040734795246No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT7720.14770907435008965No Hit
GTTTTGACTGAATACATGCATACAAGGGCTTTAAAGCAGGATAACCACAT7660.14656107636291277No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTGACCTTTGGATAAAAC7590.1452217453778731No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7580.1450304123800103No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG7560.14464774638428468No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7450.14254308340779376No Hit
CCTCACAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTC7450.14254308340779376No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT7370.1410124194248913No Hit
GTTAATAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGTCTGATGTGAT7360.14082108642702848No Hit
CAGTTAATGAGAAAAGAAGGCTGCAATTGATTATGCCTGCTAGGTTTTAT7340.14043842043130284No Hit
CCTATTAACAGGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCT7290.1394817554419888No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT7200.1377597584612235No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT7050.13488976349328136No Hit
CAAATATACATCGTATCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCC6890.13182843552747636No Hit
CCTCGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT6890.13182843552747636No Hit
CTTCTAGATACCGCCTCAGCTCTGTTTCGGGAAGCCTTAGAGTCTCCTGA6870.13144576953175074No Hit
GTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTTGC6850.13106310353602516No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6840.13087177053816232No Hit
GTTTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG6830.1306804375402995No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC6780.12972377255098547No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6750.12914977355739704No Hit
CTCCTATGCCGATCCATACTGCGGAACTCCTAGCCGCTTGTTTTGCTCGC6680.12781044257235735No Hit
GCATAGGAGCCAGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAG6670.12761910957449454No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6670.12761910957449454No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTATGGACTTCTTTCCTTCAGTACG6650.12723644357876893No Hit
TCACCATACTGCTCTCAGGCAAGCAATTCTGTGCTGGGGGGAACTAATGA6600.12627977858945488No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA6570.12570577959586646No Hit
GCCTGAGGATGAGTGTCTCTCAAAGGTGGAGACAGCGGGGTAGGCTGCCT6550.12532311360014084No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA6550.12532311360014084No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA6490.12417511561296395No Hit
GTGTAAATAATGTCTTGTTTGGAAGTAATGATTAACTAGATGTTCTGGGT6380.12207045263647304No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCCCATATCGTCAATCTTCTCG6370.12187911963861024No Hit
CTCTACTTCCAGGATCTTCAACTACCAGCACGGGACCATGCAGAACCTGC6290.12034845565570775No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAGTTCCACTGCATG6290.12034845565570775No Hit
GTCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA6250.1195831236642565No Hit
GTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAATATACATTTACA6210.11881779167280528No Hit
AGTCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGT6180.11824379267921684No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6170.11805245968135403No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6160.11786112668349122No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6110.11690446169417718No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6060.11594779670486312No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT6050.1157564637070003No Hit
GCTTTAGGGCATGGACATTGACCCTTATAAAGAATTTGGAGCTTCTGTGG6010.11499113171554906No Hit
GCCTTAGAGTCTCCTGAGCATTGTTCACCTCACCATACTGCTCTCAGGCA5940.11365180073050939No Hit
CATTGACTGTGAGTGGGCCTACAAATTGTTCACATTTTTTGATAATGTCT5940.11365180073050939No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC5850.11192980374974408No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT5840.11173847075188127No Hit
CCTCAAAATACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTC5720.10944247477752755No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5710.10925114177966476No Hit
GTCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA5690.10886847578393911No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5660.1082944767903507No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA5620.10752914479889945No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT5610.10733781180103664No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG5610.10733781180103664No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA5540.10599848081599697No Hit
GGCTATGTCATTGGATGTTATGGGTCATTGCCACAAGATCACATCAGACA5530.10580714781813416No Hit
AGTCTGTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT5500.10523314882454572No Hit
TCTCGGGGCCGCTTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCG5490.10504181582668291No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT5480.1048504828288201No Hit
GTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACTTCCAAACA5470.1046591498309573No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5410.10351115184378044No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT5390.10312848584805481No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5380.102937152850192No Hit
ATATGGGAGAGACAGTAGTCGGAACAGGGTTTACTGTTCCTGAACTGGAG5360.10255448685446639No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG5320.10178915486301514No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG5300.10140648886728951No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAAATTCCTATGGGAG5290.1012151558694267No Hit
GTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGAC5270.1008324898737011No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATAA5270.1008324898737011No Hit
GAGTAACCCCATCTTTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA5260.10064115687583829No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT5260.10064115687583829No Hit
CTCTTTACGCGGACTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5250.10044982387797546No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACCGCATCTTGAGTCCCTTTTTACC5250.10044982387797546No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG5230.10006715788224985No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)