FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488706

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488706
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences359655
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA19160.5327327577817631No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17490.48629937022980363No Hit
CCTTTTTTCATGCACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA17060.4743434680457661No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC16720.4648899639932713No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT13840.38481322378390403No Hit
TGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATG13120.3647940387315622No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT12350.34338463249503No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG12210.3394920131792968No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG11390.31669238575857417No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT11290.31391194339019335No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA11050.30723888170607944No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9990.27776619260124286No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC9930.2760979271802144No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA9250.2571909190752249No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8810.2449569726543493No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8400.23355715894398799No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC8270.22994258386509295No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8190.2277182299703883No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7540.20964535457591305No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7470.20769904491804644No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7300.2029722928917991No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC7220.20074793899709445No Hit
CCTGTTTTCATGTACTGTAAGGGGCCCCACAAATTGCTTACATCTATCAA7170.19935771781290404No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA7160.19907967357606593No Hit
GAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC7140.19852358510238977No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7090.19713336391819938No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7010.19490901002349473No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6970.1937968330761424No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6620.18406528478680959No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT6510.18100679818159068No Hit
CTCTACTTCCAGGATCATCAACAACCAGCACCGGACCATGCAAGACCTGC6330.17600200191850524No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6250.1737776480238006No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6160.17127524989225784No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6040.16793871905020089No Hit
GTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGCAA6010.16710458633968664No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6000.16682654210284856No Hit
GGTAGAGACAGTAGTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCAC5950.16543632091865815No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5940.16515827668182007No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5890.16376805549762968No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5850.16265587855027736No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5830.1620997900766012No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA5750.15987543618189654No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5630.15653890533983958No Hit
GCACCGGACCATGCAAGACCTGCACAACTCCTGCTCAAGGAACCTCTATG5580.15514868415564917No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5560.154592595681973No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG5510.1532023744977826No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT5500.1529243302609445No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5410.15042193212940178No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTCTTGCATGGTCCGGTG5380.14958779941888753No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA5350.1487536667083733No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5220.14513909162947825No Hit
GTACAATAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG5190.144304958918964No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5180.14402691468212592No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5180.14402691468212592No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5110.14208060502425937No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5110.14208060502425937No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5100.1418025607874213No Hit
GTACAGGGTCCCCAGTCTTCGATAAGGTTGACGATATGGTAGAGACAGTA5080.14124647231374513No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5060.14069038384006896No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5040.1401342953663928No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT4980.1384660299453643No Hit
CTCTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGC4980.1384660299453643No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT4980.1384660299453643No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4970.13818798570852622No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4970.13818798570852622No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4940.13735385299801198No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4920.1367977645243358No Hit
GTATGGATCGGCAGCGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4910.13651972028749773No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGGTT4910.13651972028749773No Hit
ATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCT4840.13457341062963118No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC4790.13318318944544077No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA4760.13234905673492653No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC4680.1301247028402219No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4670.12984665860338382No Hit
GCTCAGACTACTGTCTCTACCATATCGTCAACCTTATCGAAGACTGGGGA4670.12984665860338382No Hit
GTCTCTACCATATCGTCAACCTTATCGAAGACTGGGGACCCTGTACCGAA4630.12873448165603146No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG4630.12873448165603146No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG4600.12790034894551722No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT4600.12790034894551722No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGCATACAAGCAAAACAG4590.12762230470867914No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT4570.127066216235003No Hit
TCTACTTCCAGGATCATCAACAACCAGCACCGGACCATGCAAGACCTGCA4520.1256759950508126No Hit
GGGAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGA4500.12511990657713642No Hit
AGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGGTGGAGGCAGGAG4460.12400772962978408No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4450.12372968539294603No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA4420.12289555268243178No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA4390.12206141997191752No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4380.12178337573507944No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4340.12067119878772714No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC4310.1198370660772129No Hit
CCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAA4280.11900293336669863No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA4260.11844684489302247No Hit
AGTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT4260.11844684489302247No Hit
CCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCTTCCTGACTGCCGATTGG4230.11761271218250825No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4220.11733466794567017No Hit
CCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAAC4220.11733466794567017No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT4160.11566640252464166No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4100.11399813710361319No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA4090.1137200928667751No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTAGCCCGAATGCTCCCA4080.11344204862993702No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG4030.11205182744574661No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATACTCTGGAT4020.11177378320890853No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG4020.11177378320890853No Hit
GAATATGGTGACCCGCAAAATGAGGCGCTACGTGTGGACTCTCTTTTATA3960.11010551778788005No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT3920.10899334084052772No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT3920.10899334084052772No Hit
ACATTATTTACACACTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGT3900.10843725236685156No Hit
GTACAGGGCCCTGACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGG3890.10815920813001349No Hit
CCCCATGGGGGACTGTTGGGGTGGAGCCCTCAGGCTCAGGGCCTACTCAC3880.10788116389317541No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA3880.10788116389317541No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA3880.10788116389317541No Hit
GCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC3860.10732507541949925No Hit
CAGTTATATGGATGATGTGGTTTTGGGGGCCAAGTCTGTACAACATCTTG3860.10732507541949925No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT3860.10732507541949925No Hit
TCCTTGTACGGGTTGAGGTCCCAATCTGGATTTTCTGAGTTGGCTTTGAA3860.10732507541949925No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGAAGAGGC3850.10704703118266116No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG3830.106490942708985No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC3810.10593485423530884No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3790.10537876576163267No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT3790.10537876576163267No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG3780.1051007215247946No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG3720.10343245610376611No Hit
CCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAAGGGACTCAAGATG3720.10343245610376611No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA3710.10315441186692803No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA3700.10287636763008995No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC3700.10287636763008995No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA3690.10259832339325187No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG3680.10232027915641377No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT3610.10037396949854722No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)