FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488708

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488708
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430849
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA43941.019846860501011No Hit
CCTCCAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA34600.8030655751783108No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA28260.6559142530213602No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA25810.5990497831026648No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT20880.4846245436336164No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT20370.47278744989543897No Hit
ATGTAAGACCTTGGGCAAGACTTGTCGAGCGTTCACGGTGGTCTCCATGC15020.3486140155831857No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG14450.3353843225816934No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14040.3258682276157076No Hit
ACTTCTCGGTACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT13580.315191633263626No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC12420.2882680475062029No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG12300.2854828489795729No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT11850.2750383545047105No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCTTATGTAAGATCTA11680.27109265659198467No Hit
GTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10720.24881106837894482No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC10500.2437048710801232No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG10420.24184807206236988No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10220.2372060745179866No Hit
CATGTATACAAGCTAAACAAGCTTTCACTTTCTCGCCAACTTACAAGACT10200.23674187476354827No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9920.23024307820141166No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG9710.22536898077980916No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG9540.22142328286708338No Hit
CCTTCTTTCGTTAACTGTAAGCGGGCCTACATATTGTTGACATCTATTGA9380.21770968483157674No Hit
AGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA9050.21005038888334426No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8830.20494419158452265No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA8620.20007009416292018No Hit
CCGATAAGTTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCTGCTAGGAG8380.19449969710966022No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8160.1893934998108386No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG8120.18846510030196195No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8070.18730460091586612No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7920.18382310275757863No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7480.17361070815993537No Hit
CGCAAGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTC7400.17175390914218205No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC7380.17128970938774374No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCAGCGGACGACCCGTCTCG7370.17105760951052457No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7330.1701292100016479No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT7300.16943291036999042No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA7120.16525511258004544No Hit
GTCTACGTCCCGTCGGCGCTGAATCCAGCGGACGACCCGTCTCGGGGCCG7110.1650230127028263No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6990.1622378141761963No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6960.1615415145445388No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT6700.15550691773684053No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA6690.15527481785962136No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAGTCTTGTAAGTTGGCGA6670.15481061810518304No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAAGCTTTCACTTT6620.1536501187190872No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAGTGTTTGCTG6600.15318591896464887No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTTGTCGAGCGTTC6590.1529538190874297No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT6560.1522575194557722No Hit
GCTCTGTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6440.14947232092914223No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6430.14924022105192306No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6300.1462229226480739No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6260.14529452313919727No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6250.1450624232619781No Hit
GCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAAC6230.14459822350753976No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAGTG6120.14204512485812895No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6010.13949202620871815No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5970.13856362669984146No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5940.13786732706818397No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5930.13763522719096483No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5910.13717102743652648No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5890.13670682768208814No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5890.13670682768208814No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5800.13461792878711568No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5790.1343858289098965No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5690.13206483013770484No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5610.13020803111995152No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5600.12997593124273238No Hit
CTCTAAGGCCTCTCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA5600.12997593124273238No Hit
CGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTC5560.1290475317338557No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5520.12811913222497906No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5510.1278870323477599No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5450.12649443308444488No Hit
AATACGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCTGTCT5430.12603023333000657No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGG5370.1246376340666916No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAACG5300.12301293492615742No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG5300.12301293492615742No Hit
GTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATAC5260.12208453541728076No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5260.12208453541728076No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA5190.12045983627674661No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACAC5180.12022773639952745No Hit
GTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5180.12022773639952745No Hit
TCTCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAA5100.11837093738177414No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT5020.11651413836402082No Hit
GTATGCATGTATACAAGCTAAACAAGCTTTCACTTTCTCGCCAACTTACA5010.11628203848680163No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4960.11512153910070583No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4900.11372893983739082No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG4870.11303264020573332No Hit
CTGCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGT4870.11303264020573332No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4860.11280054032851417No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4860.11280054032851417No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGTGATCTCCTCGACACC4810.11164004094241833No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4800.11140794106519918No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4790.11117584118798No Hit
CTTATGTAAGATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATAC4790.11117584118798No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4780.11094374131076085No Hit
CTCTTATGTAAGACCTTGGGCAAGACTTGTCGAGCGTTCACGGTGGTCTC4730.10978324192466501No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4730.10978324192466501No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCTCGATACAG4710.10931904217022669No Hit
GGGCAAGACTTGTCGAGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG4690.10885484241578837No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4680.10862274253856918No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4680.10862274253856918No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC4640.10769434302969254No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA4620.10723014327525421No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4620.10723014327525421No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT4600.10676594352081586No Hit
GTATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGGTCTTC4590.1065338436435967No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4480.1039807449941859No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT4480.1039807449941859No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4470.10374864511696674No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC4460.10351654523974756No Hit
ATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAATAGAAGGAAAGAAGT4420.10258814573087091No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4410.10235604585365175No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCC4400.10212394597643257No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4390.10189184609921342No Hit
CCTCTGCACGTCGCATGGAGACCACCGTGAACGCTCGACAAGTCTTGCCC4340.10073134671311759No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTG4330.10049924683589843No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)