FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488709

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488709
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430849
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA37500.8703745395718686No Hit
CCTCCAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA34510.8009766762833384No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA24210.5619138027475984No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA23600.5477557102372294No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT19630.4556120589812208No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT19240.44656016376967334No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG15490.35952270981248646No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG14530.3372411215994467No Hit
CCTCAAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13350.30985333608758525No Hit
ACTTCTCGGTACCGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT13310.30892493657870856No Hit
ATGTAAGACCTTGGGCAAGACTTGTCGAGCGTTCACGGTGGTCTCCATGC12420.2882680475062029No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC11760.272949455609738No Hit
CATTTAAACCCTACTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT11760.272949455609738No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC11210.26018396236268393No Hit
GTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10700.2483468686245065No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCTTATGTAAGATCTA10550.24486537046621903No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10350.24022337292183574No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG10210.2369739746407674No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG9540.22142328286708338No Hit
CCTTCTTTCGTTAACTGTAAGCGGGCCTACATATTGTTGACATCTATTGA9490.22026278348098752No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9360.21724548507713837No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9310.21608498569104256No Hit
CATGTATACAAGCTAAACAAGCTTTCACTTTCTCGCCAACTTACAAGACT9300.2158528858138234No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA8740.20285529268955016No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG8640.2005342939173585No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG8400.19496389686409857No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG8330.1933391977235644No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8290.19241079821468776No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCAGCGGACGACCCGTCTCG8220.1907860990741536No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7650.1775564060726612No Hit
GTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7650.1775564060726612No Hit
GTCTACGTCCCGTCGGCGCTGAATCCAGCGGACGACCCGTCTCGGGGCCG7590.17616380680934618No Hit
AGCTTGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA7550.17523540730046955No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA7320.16989711012442874No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAATATGTT7200.16711191159779876No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7200.16711191159779876No Hit
CTGCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGT6880.15968471552678548No Hit
CGCAAGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTC6820.15829211626347048No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAGTGTTTGCTG6690.15527481785962136No Hit
CCGATAAGTTTTGCTCCAGACCTGCTGCGAGCAAAACAAGCTGCTAGGAG6640.15411431847352552No Hit
GCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAAC6600.15318591896464887No Hit
AGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAA6570.15248961933299138No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6550.15202541957855303No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT6540.15179331970133386No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6450.1497044208063614No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6390.14831182154304642No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACTTGTCGAGCGTTC6180.14343772412144393No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAAGTCTTGTAAGTTGGCGA6080.1411167253492523No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5970.13856362669984146No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5960.1383315268226223No Hit
GCTCTGTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA5880.136474727804869No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTGTGCCAAGTG5850.1357784281732115No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5810.13485002866433485No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAAGCTTTCACTTT5800.13461792878711568No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5780.13415372903267733No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5750.13345742940101984No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5710.13252902989214319No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5680.1318327302604857No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATA5630.13067223087438987No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5630.13067223087438987No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5550.12881543185663655No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5530.1283512321021982No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC5520.12811913222497906No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC5470.12695863283888323No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5460.12672653296166406No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5410.12556603357556823No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT5380.12486973394391075No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5380.12486973394391075No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5350.12417343431225325No Hit
CTCTAAGGCCTCTCGATACAGAGCAGAGGCGGTGTCGAGGAGATCACGAA5330.12370923455781492No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGG5320.12347713468059576No Hit
ACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT5250.1218524355400616No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5150.11953143676786994No Hit
GTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG5090.11813883750455495No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCGCTTACAGTTAACG5030.11674623824123997No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4950.11488943922348664No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4940.11465733934626748No Hit
CGCATGCGCTGATGGCCTATGGCCAAGCCCCAACCAGTGGGGGTTGCGTC4900.11372893983739082No Hit
GTATGCATGTATACAAGCTAAACAAGCTTTCACTTTCTCGCCAACTTACA4860.11280054032851417No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4810.11164004094241833No Hit
GACCTGACCGTTGCCGAGCAACGGGGTAAAGGTTCAGATATTGTTTACAC4790.11117584118798No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGT4690.10885484241578837No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4660.10815854278413087No Hit
CCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGACCCGTAT4650.1079264429069117No Hit
CTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCGTGATCTCCTCGACACC4640.10769434302969254No Hit
AATACGGGCCTAAAAATTAGACAACTATTGTGGTTTCACATTTCCTGTCT4610.10699804339803505No Hit
GTATGGTGAGGTGAACAATGTTCCGGAGACTCTAAGGCCTCTCGATACAG4600.10676594352081586No Hit
GGGCAAGACTTGTCGAGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG4600.10676594352081586No Hit
CTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACGGGGCGCACCT4590.1065338436435967No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4590.1065338436435967No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC4580.10630174376637755No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGTACAAT4570.10606964388915838No Hit
GTATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGGTCTTC4560.10583754401193922No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT4550.10560544413472006No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4540.10537334425750088No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4500.10444494474862423No Hit
GTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGTAGGGTTTAAATGTATAC4480.1039807449941859No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4460.10351654523974756No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4440.10305234548530924No Hit
TCTCGGGGCCGTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCG4430.10282024560809007No Hit
GCGCTTGCGTGGAACCTTTGTGGCTCCTCTGCCGATCCATACTGCGGAAC4430.10282024560809007No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4420.10258814573087091No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCC4360.10119554646755592No Hit
GGAACATATTGTACACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA4350.10096344659033675No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)