FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434212
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTAAATACACAGTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACA26680.6144463994546443No Hit
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT19880.4578408703582582No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT19030.43826517922120994No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT18850.4341197387451291No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17090.39358654297900564No Hit
GTCTGTAAATACACAGTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACA15800.3638775529004265No Hit
CATGTATACATGCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC14750.33969581678995514No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG13260.3053807817379529No Hit
GTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12980.2989323187751605No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12180.280508138881468No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTATTCCCTTCACTTCAT12150.27981723213545456No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG11590.26692030620986984No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11350.26139305224176207No Hit
GTAAATACACAGTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACATTGC10600.2441203835914254No Hit
CTCCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG10050.23145375991451184No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA10030.2309931554171695No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGG10030.2309931554171695No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT9980.22984164417381373No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA9970.22961134192514254No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT9530.2194780429836117No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC9170.21118716203145008No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA9030.20796293055005388No Hit
GCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC8990.20704172155536926No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC8580.1975993293598519No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACCACAGAGT8510.19598721361915378No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8170.1881569371643345No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCTAAAGATTAATCTA8020.18470240343426714No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG8000.18424179893692483No Hit
GCACGGGACCATGCAAGACCTGCACGAATCCTGCTCAAGGAACCTCTATG7920.18239938094755556No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7490.17249638425469585No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC7310.16835094377861506No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7220.16627822354057464No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC7160.16489641004854771No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7120.1639752010538631No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT7100.16351459655652079No Hit
ACATAGAGGTTCCTTGAGCAGGATTCGTGCAGGTCTTGCATGGTCCCGTG7100.16351459655652079No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6890.1586782493344265No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG6880.15844794708575535No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGCACAATATGTT6780.15614492459904378No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6700.15430250660967454No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6690.15407220436100338No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG6630.15269039086897646No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6620.1524600886203053No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6530.15038736838226488No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6500.14969646163625142No Hit
AAGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA6410.14762374139821102No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC6400.14739343914953984No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6360.14647223015485522No Hit
ATCTAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGG6340.1460116256575129No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6340.1460116256575129No Hit
ATATAAGAGAGAAACTACTCGTAGCGCCTCATTTTGTGGGTCACCATATT6230.1434783009221302No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6180.14232678967877443No Hit
GCATTAATGCCTTTGTATGCATGTATACATGCCAAGCAGGCTTTCACTTT6070.1397934649433917No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6050.1393328604460494No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6030.13887225594870709No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5920.13633893121332438No Hit
GGAACATATTGTGCACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA5890.1356480244673109No Hit
TCCTTTGTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCG5890.1356480244673109No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG5800.1335753042292705No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5740.13219349073724354No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5670.13058137499654546No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5670.13058137499654546No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC5660.13035107274787433No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGG5630.12966016600186084No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5520.12712684126647814No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5450.12551472552578005No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5420.12482381877976657No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5380.12390260978508195No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5350.12321170303906848No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5260.12113898280102808No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5260.12113898280102808No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5190.11952686706032999No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT5190.11952686706032999No Hit
CTCCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5170.11906626256298766No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGCACAAT5170.11906626256298766No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAC5150.11860565806564535No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5100.11745414682228957No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5000.11515112433557802No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGTAGCGCCTCAT4980.1146905198382357No Hit
TTCTATTCGTGATCTCCTCGACACCGCCTCTGCTCTATATCGGGAGGCCT4960.11422991534089338No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4920.11330870634620877No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4920.11330870634620877No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4910.1130784040975376No Hit
ATCCTAACCGTACAAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4860.11192689285418184No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4850.11169659060551067No Hit
TGCAAGACCTGCACGAATCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4840.11146628835683951No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG4820.1110056838594972No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTTAC4810.11077538161082606No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4790.11031477711348373No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGTAGCGCC4730.1089329636214568No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCATG4720.10870266137278564No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT4720.10870266137278564No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4670.10755115012942987No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4630.10662994113474523No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTTGTACGGTT4620.1063996388860741No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT4610.10616933663740293No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4600.10593903438873176No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGT4580.10547842989138945No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4570.1052481276427183No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4550.104787523145376No Hit
GTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4520.10409661639936253No Hit
AACATATTGTGCACAAAATCAAACAATGTTTTCGGAAACTTCCTATAAAT4500.10363601190202022No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGCC4490.10340570965334905No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC4490.10340570965334905No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCTGACGGG4450.10248450065866443No Hit
GCACCAAACCTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCT4450.10248450065866443No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA4440.10225419840999327No Hit
TATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGGTCTTCC4400.10133298941530865No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG4370.10064208266929518No Hit
AGTCTGTACAACATCTTGAATCCCTTTTTACCGCTGTTACCAATTTTCTT4350.10018147817195287No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)