FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488711

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488711
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences434212
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATTTAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT19150.4410288062052638No Hit
GTCTTTAAATACACAGTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACA18870.43458034324247147No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACATACT17290.39819258795242873No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT17070.3931259384816634No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15290.35213213821819755No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG13660.3145928716847991No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12260.2823505568708373No Hit
GTCTGTAAATACACAGTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACA12140.2795869298867834No Hit
CATGTATACATGCCAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC11910.2742899781673468No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG11890.27382937367000454No Hit
CATTTAAACCCTACTAAAACCAAACGTTGGGGCTATTCCCTTCACTTCAT10380.23905373412065997No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGG9960.2293810396764714No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9850.22684771494108869No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA9600.2210901587243098No Hit
GTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9590.2208598564756386No Hit
CTCCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG9310.21441139351284624No Hit
GCTCTATATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA9120.21003565078809427No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA8500.19575691137048262No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT8320.1916114708944018No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT7940.18285998544489787No Hit
GCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC7680.17687212697944782No Hit
GCACGGGACCATGCAAGACCTGCACGAATCCTGCTCAAGGAACCTCTATG7530.1734175932493805No Hit
GTAAATACACAGTCTTTGAAATATGCCTCAAGGTCGGTCGTTGACATTGC7520.17318729100070934No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7430.17111457076266892No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7070.16282368981050732No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC7050.162363085313165No Hit
TCCTTTGTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCG6950.16006006282645344No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6800.1566055290963861No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG6800.1566055290963861No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG6790.15637522684771493No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACCACAGAGT6780.15614492459904378No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG6720.15476311110701685No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6660.1533812976149899No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6580.15153887962562068No Hit
CCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6530.15038736838226488No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6380.14693283465219753No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6360.14647223015485522No Hit
GTCTACGTCCCGTCAGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6310.14532071891149945No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCTAAAGATTAATCTA6140.1414055806840898No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTGTGCACAATATGTT6090.140254069440734No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6060.13956316269472055No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG6020.13864195370003593No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6020.13864195370003593No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6020.13864195370003593No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCGTTAAC5990.13795104695402247No Hit
ATCTAAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAATTTTACTGGG5980.1377207447053513No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG5890.1356480244673109No Hit
GGAACATATTGTGCACAAAATCAAACAATGTTTTCGGAAACTTCCTATAA5800.1335753042292705No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT5750.13242379298591472No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5740.13219349073724354No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5720.13173288623990123No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5670.13058137499654546No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTTTCCATGC5630.12966016600186084No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC5630.12966016600186084No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5550.1278177480124916No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5550.1278177480124916No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5530.1273571435151493No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5480.1262056322717935No Hit
ACATAGAGGTTCCTTGAGCAGGATTCGTGCAGGTCTTGCATGGTCCCGTG5450.12551472552578005No Hit
GCATTAATGCCTTTGTATGCATGTATACATGCCAAGCAGGCTTTCACTTT5430.12505412102843771No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5420.12482381877976657No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5360.12344200528773962No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5340.12298140079039731No Hit
CTCCTACTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5240.12067837830368575No Hit
AAGCAGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA5240.12067837830368575No Hit
ATATAAGAGAGAAACTACTCGTAGCGCCTCATTTTGTGGGTCACCATATT5110.11768444907096073No Hit
CAATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATG5110.11768444907096073No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5090.11722384457361842No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5080.11699354232494726No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGGCCGACCACG5080.11699354232494726No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA5040.11607233333026264No Hit
CTCCTACCTGGTGTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC4990.11492082208690686No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4940.11376931084355107No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG4940.11376931084355107No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTCTGG4900.11284810184886646No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4860.11192689285418184No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4810.11077538161082606No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4710.10847235912411449No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCA4680.10778145237810102No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4680.10778145237810102No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4620.1063996388860741No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT4580.10547842989138945No Hit
ATCCTAACCGTACAAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT4530.10432691864803367No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4520.10409661639936253No Hit
ACTCTGTGGTCCTCTCTCGGAAATACACCTCCTTTCCATGGCTGCTAGGC4500.10363601190202022No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4470.10294510515600674No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTTAC4460.10271480290733558No Hit
TTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC4460.10271480290733558No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA4430.10202389616132213No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4360.10041178042062404No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)