FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488712

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488712
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences417961
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA26100.6244601769064577No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA18690.44717090829048645No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16170.38687820155469055No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC15470.3701302274614139No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14720.3521859695043318No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC13330.3189292780905395No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC12270.29356806017786347No Hit
GTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCTCCCGC11770.2816052215398087No Hit
GTGTAAAAGGGGCGGCAAATCCCAGAAGACCCACAATTCTTTGACATACT10960.26222542294616No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT10770.2576795442636992No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG10190.24380265144355573No Hit
CTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTCCTCAC9920.23734271857900618No Hit
GTTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA9490.2270546773502791No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9330.22322656898610158No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9140.21868069030364076No Hit
GTATAGGCCTCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG8790.21030670325700246No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTGTTGATAATGTCCTCTTTC8730.20887116262043587No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8500.2033682568469307No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA8190.19595129689133675No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8160.19523352657305348No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8130.1945157562547702No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG8060.19284095884544253No Hit
ATCCTAAACTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT7980.19092690466335377No Hit
CTTTTACACAATGTGGTTACCCAGCATTAATGCCTTTATATGCATGTATA7830.18733805307193735No Hit
GTATACATGCATATAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAA7770.18590251243537076No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG7750.1854239988898486No Hit
GTAGTAAGCTATGTCAATGTTAACATGGGCCTAAAAATCAGACAACTATT7690.183988458253282No Hit
GCATTAATGCCTTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT7610.18207440407119324No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG7560.18087812020738778No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7540.18039960666186558No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG7510.1796818363435823No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG7170.17154710606970508No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG7150.17106859252418288No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC7130.1705900789786607No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7090.16963305188761632No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC6970.16676197061448317No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT6910.16532642997791658No Hit
CCTCAGCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAGTCTTTGA6830.16341237579582782No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC6780.16221609193202235No Hit
ATTTATAGGAAGTTTCCGAAAACATAGTTTCAATTTTTGTACAATATGTT6690.1600627809771725No Hit
GGAACATATTGTACAAAAATTGAAACTATGTTTTCGGAAACTTCCTATAA6670.1595842674316503No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACACGCAGTGCC6670.1595842674316503No Hit
TCCACAGAGTATGCAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTA6610.15814872679508374No Hit
CTCTAGAACAGTTTCTCTTCCAAAAGTAAGACAGGAAATGTGAAACCACA6580.15743095647680047No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6580.15743095647680047No Hit
ATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATATT6510.15575615906747278No Hit
TTCCATGGCTGCTCGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6450.15432061843090622No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6450.15432061843090622No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC6440.15408136165814512No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6400.15312433456710076No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6370.15240656424881746No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6270.1500139965212065No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6130.14666440170255118No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6060.14498960429322352No Hit
GCTTACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCC5910.1414007527018071No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT5900.141161495929046No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5830.13948669851971834No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTCTGG5780.13829041465591288No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5710.1366156172465852No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5700.13637636047382412No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5660.13541933338277973No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5640.13494081983725753No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5630.13470156306449643No Hit
ACTACACGCAGTGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA5590.13374453597345207No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5580.13350527920069097No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5580.13350527920069097No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5570.13326602242792987No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5540.1325482521096466No Hit
GTATGCAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG5510.1318304817913633No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5490.1313519682458411No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5470.13087345470031891No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCG5440.13015568438203565No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5440.13015568438203565No Hit
ATTTGCATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA5420.12967717083651345No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5410.12943791406375235No Hit
GGCCTACATATTGTTGACATCTGTTGATAATGTCCTCTTTCAAATGAATG5360.1282416301999469No Hit
GGCCTATACTTTCCTGCTGGTGGCTCAAGTTCCGGAACAGTAAACCCTGT5360.1282416301999469No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5340.1277631166544247No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5340.1277631166544247No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATG5330.1275238598816636No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5320.1272846031089025No Hit
GAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGATACTAAC5300.12680608956338033No Hit
TTATTATCCTGAACATACAGTTAATCATTACTTCCAAACTAGACATTATT5250.12560980569957483No Hit
GTATCGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5250.12560980569957483No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACACGCAGTGCCTCAT5200.12441352183576937No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT5190.12417426506300827No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5190.12417426506300827No Hit
GCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCGGTG5170.12369575151748607No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5150.12321723797196389No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5110.12226021088091951No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTAAGTTT5110.12226021088091951No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC5090.12178169733539732No Hit
TGCATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACACGC4960.11867135928950309No Hit
CCCCAAAAGATTGCCGTGGGGTGGAGTGAACCCTGGCCCGAATGATCCCA4950.118432102516742No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4950.118432102516742No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACGG4940.1181928457439809No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG4940.1181928457439809No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4870.11651804833465323No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4870.11651804833465323No Hit
ATGTTAACATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4850.11603953478913104No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATAGTTTCAATTTTTGTACAAT4810.11508250769808666No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC4800.11484325092532557No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC4790.11460399415256448No Hit
TCACCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4770.11412548060704228No Hit
AAACTGTTCTAGAGTATTTGGTGTCTTTTGGAGTGTGGATTCGCACTCCT4760.1138862238342812No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG4750.1136469670615201No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4690.11221142642495352No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4690.11221142642495352No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACCTTT4650.11125439933390914No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC4620.11053662901562586No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4600.11005811547010368No Hit
CCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATG4590.10981885869734258No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCATCTCGGGGCCG4560.1091010883790593No Hit
TTATATAAGAGAGAAACTACACGCAGTGCCTCATTTTGTGGGTCACCATA4520.10814406128801492No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4480.10718703419697054No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4460.10670852065144834No Hit
ATATGGGAGAGGCAGTGGTCGGAACAGGGTTTACTGTTCCGGAACTTGAG4460.10670852065144834No Hit
CTTATATAAAATACCAGCCTTCCACAGAGTATGCAAATAATGTCTAGTTT4460.10670852065144834No Hit
GTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA4420.10575149356040396No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4410.10551223678764286No Hit
CTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCGGGATCTCCTCGACACC4400.10527298001488178No Hit
TTCTTCTACTGTACCTGTCTTTAATCCTGAATGGCAAACTCCCTCTTTTC4370.10455520969659848No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4350.1040766961510763No Hit
GGGTTTACTGTTCCGGAACTTGAGCCACCAGCAGGAAAGTATAGGCCTCT4310.10311966906003192No Hit
GAGTGAACCCTGGCCCGAATGATCCCACTCCTACCTGGTTTGCCGCTGGC4280.10240189874174864No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG4260.10192338519622644No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTGCCAGC4260.10192338519622644No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG4260.10192338519622644No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC4220.10096635810518206No Hit
CTCTTATATAAAATACCAGCCTTCCACAGAGTATGCAAATAATGTCTAGT4210.10072710133242097No Hit
CTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTGTACCTGTC4200.10048784455965988No Hit
CTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTC4190.10024858778689878No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCGAGCAGCC4180.10000933101413768No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)