FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488715

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488715
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences532221
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC26510.4981013526335864No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGAAATACCCGTG18440.34647261194128004No Hit
GTCCGGTGCTGGTCGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC17950.33726591021399005No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT17580.33031391095052615No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC16440.3088942375441781No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG16110.30269380576865623No Hit
AGATTGAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCG15650.2940507796573228No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCACAAGGAACCTCTATG14690.2760131599467139No Hit
CTCAATAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA14370.27000062004317754No Hit
TTGTACACCTCACCATACGGCACTCAGGCAAGCTATTCTGTGTTGGGGTG14110.2651154313715543No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA14030.2636122963956702No Hit
TCTCCATCTCGGGGATCCCAATGTTAGTATTCCTTGGACACATAAGGTGG13890.2609818101878731No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC13540.25440559466838025No Hit
TCTGCTGAGTTACTCTCTTTTTTGCCTACTGACTTCTATCCTAATGTTCG13290.2497082978687425No Hit
ATCTCCAGTCACTCACTAACCTGTTGTCCTCCAATTTGTCCTGGCTATCG13210.2482051628928584No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA12850.24144105550138006No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA12150.2282886244623944No Hit
GTCTTGAAGTAATGATTAACTACATGCTCTGGATAATAAGGTTTGATCCC11860.2228397601748146No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA11180.21006311287979992No Hit
GGCAGAGACAGTATTCTGAGCAGGGCTTACTGTTCCTGAACTGGAGCCAC10850.2038626811042781No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT10290.19334073627308956No Hit
TAGAAAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC9760.18338246705785755No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA9720.18263089956991552No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAAGCCATGCAGTGGAA9380.1762425759224082No Hit
GTATCCAGTTGGCAGCACAGCCTAGCAGCCATGGAAAGGATGTATACTTG9190.1726726303546835No Hit
ACTGGATACTGCGCGGGACGTCCTTTGTCTACGTCCCGTCGGCGCTGAAT8990.16891479291497327No Hit
GGGATAAAACCTAGCAGGCATAATTAATTTTAGTCTTCTTTTTTCGTTTA8980.1687269010429878No Hit
CTCACCTGCCGGACCGTGTGCACTTCGCTTCACCTCTGCACGTCGCATGG8780.1649690636032776No Hit
CCTCTAAGGGACACTCATCCTCAAGCCATGCAGTGGAACTCCACCACTTT8720.16384171237136452No Hit
GTCTCAGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA8690.163278036755408No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8550.16064755054761087No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8490.1595201993156978No Hit
CTTTTTCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC8480.1593323074437123No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8330.15651392936392963No Hit
TCCTCAAGCCATGCAGTGGAACTCCACCACTTTTCACCAAACTCTACAAG8250.15501079438804555No Hit
CTCCAAAAGAAACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA8150.15313187566819048No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC8130.15275609192421946No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT8120.15256820005223393No Hit
ATTCTGAGCAGGGCTTACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT8030.15087717320436433No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC7870.1478709032525962No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT7870.1478709032525962No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATACTGC7670.14411306581288602No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG7490.1407310121171468No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT7430.13960366088523377No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7410.13922787714126275No Hit
CTCGTTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7360.1382884177813352No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7170.13471847221361052No Hit
CTTTATCTAAGGGCAAATATTTAGTAACATTGGGATAAAACCTAGCAGGC7030.13208798600581337No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG7020.13190009413382786No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7000.13152431038985685No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA6990.13133641851787134No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6960.1307727429019148No Hit
ACTGCAGATTTGGGACTGCGAATTTTGGCCAAGACACACGGGTATTTCCC6940.13039695915794378No Hit
CTCTGTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC6830.12833014856610317No Hit
CTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATAC6820.12814225669411766No Hit
CTGCTAGGCTGTGCTGCCAACTGGATACTGCGCGGGACGTCCTTTGTCTA6780.12739068920617563No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTACTGACTTCTTTCCTAATGTTCG6700.12588755423029155No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACGACC6680.12551177048632053No Hit
GAACCTGCCCAAGGTCTTGCATAAGAGGACTCTTGGACTTTCAGCAATGT6620.12438441925440746No Hit
CTACAAGATCCCAGAGTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAG6550.12306917615050891No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC6540.12288128427852339No Hit
CTTCAAGACAAGACATTATTTACACACTCTTTGGAAGGCGGGTATCTTAT6540.12288128427852339No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6500.12212971679058135No Hit
TACACACTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGT6460.12137814930263931No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG6390.12006290619874076No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6370.11968712245476973No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG6360.11949923058278422No Hit
ACATAGAGGTTCCTTGTGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTG6350.11931133871079871No Hit
GTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG6340.11912344683881321No Hit
GTCTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCCTCCCGGGGCCG6310.11855977122285667No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6260.11762031186292914No Hit
GGTATTTCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG6240.1172445281189581No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTCCCACGCATGCGCTGATGGCC6190.11630506875903056No Hit
GTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAA6180.11611717688704505No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGGCCCACAATTCG6150.11555350127108852No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC6080.11423825816718994No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGGCCCACAAT6070.11405036629520443No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCAGATACTGTTTACTT6030.1132987988072624No Hit
GGCAAGTTCCGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGT6000.11273512319130585No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5960.11198355570336382No Hit
GTGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAGGTAATTTGC5940.11160777195939281No Hit
CTCCAAAAGATACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA5880.11048042072747974No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5850.10991674511152323No Hit
CTCAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA5840.10972885323953772No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA5740.10784993451968261No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5650.10615890767181303No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCTGCGGACGACCCCTCCCG5650.10615890767181303No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACAAAATGCC5620.1055952320558565No Hit
GTTGACTACTGCTTCCCTGGATGCTTGATCTTGCAAATTACCTCCCACCC5610.10540734018387099No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5610.10540734018387099No Hit
CTTGACTCCCTTTATGCCGCTGTTACCAATTTTCTTTTGTCTTTGGGTAT5590.10503155643989996No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5580.10484366456791445No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAGA5550.10427998895195793No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTGCTGTTATTAG5510.10352842146401589No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGGACCTTTGTGTC5500.10334052959203037No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGA5500.10334052959203037No Hit
CTTCCAGGCAAGACATTATTTACACACTCTTTGGAAGGCGGGTATCTTAT5470.10277685397607386No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5470.10277685397607386No Hit
GTGCAGGAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTCGTTGATGAT5450.10240107023210282No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAATACG5430.10202528648813182No Hit
GGGTTCACCCCTCCCCATGGGGGACTGTTGGGGTGGAGCCCTCAGGCTCA5420.1018373946161463No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5400.10146161087217528No Hit
GTAGTCAACTATGTCAACGTTAATATGGGCATAAAAATCAGACAACTATT5390.10127371900018978No Hit
TCTCAATCTCGGGGATCCCAATGTTAGTATTCCTTGGACACATAAGGTGG5380.10108582712820426No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACTT5380.10108582712820426No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5360.10071004338423324No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5350.10052215151224773No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5340.10033425964026221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)