FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488716

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488716
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536534
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC28520.5315599756958552No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG21660.4037022816820556No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA13580.2531060473334402No Hit
TTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCGGGGC11260.20986554440165953No Hit
GTCCCGTACTGGTTGTTGTTGATCATTGAGAGTCCCGAGATTGAGATCTT10840.202037522319182No Hit
GCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGCGCCCC10460.19495502614932136No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA10360.19309121136777913No Hit
CTCCAAAAGAGACCAAATATTCAAGTACAGTTTCTCTTCCAAAAGTAAGG10270.19141377806439105No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT10050.18731338554499807No Hit
CCTCCACCTTATAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA9760.1819083226785255No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9350.1742666820742022No Hit
ACTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCCGTCAG9270.17277563024896836No Hit
GGACTCTATCGTCCCCTTCTCCGTCTGCCGTTCCAGCCGACCACGGGGCG8910.1660658970354162No Hit
GTATTAGGCTGTTCTTGCATTGCTATAAGGAAATACCTGAGACTGGGCAA8560.15954254530001827No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC8280.15432386391169992No Hit
TCCTTGCACCATATAAAAATCAACTCAAAATGGATTAAAGACTTAAATGA8150.15190090469569495No Hit
ACCTGAGACTGGGCAATATTTAAAGAAAAGAGGTTTTTGGCTCAAGGCTC8000.14910518252338156No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCACCTTATAGACCAGCAAATGCC7990.14891880104522734No Hit
GCCTTAGAGTCTCCTGAGCATTGCACACCTCACCATACTGCACTCAGGCA7340.1368040049652026No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7230.13475380870550607No Hit
CTCACCTGCCGGTCCGTGTGCACTTCGCTTCACCTCTGCACGTTGCATGG7090.1321444680113469No Hit
GTACAGGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG6990.13028065322980464No Hit
TCAAAGACCACTGGCCAGCAGCCAACCAGGTAGGAGTGGGAGCATTCGGG6800.12673940514487433No Hit
CCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCATGGGTTACA6350.11835223862793412No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6340.11816585714977988No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA6300.11742033123716299No Hit
GTGTGAGACGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTGATGGTT6050.11276079428330729No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6050.11276079428330729No Hit
ATGGAATACTATGCAGCCCTAAAAATTGAAATCATGCCTTTTGCAGCAAC5950.11089697950176503No Hit
GACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTCCTGTACTGTTGACTT5900.10996507211099389No Hit
ATACTATGCAGCCCTAAAAATTGAAATCATGCCTTTTGCAGCAACATGGA5890.10977869063283968No Hit
TTGTTCACACCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGG5810.10828763880760585No Hit
ATAAAGCCCCAAGCGGCCCCGAGAGGGGTCGTCCGCGGGATTCAGCGCCG5790.1079148758512974No Hit
CCTCCACCCTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5790.1079148758512974No Hit
CCTGTATTCCCATCCCATCGTCCTGGGCTTTCGCAAAATACCTATGGGAG5610.10456000924452132No Hit
GTGCTGGTGCAAAACCAGACACATAGATCAATGGAACAGGTTACAGAACA5510.10269619446297905No Hit
ATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTATACAAGCTAAAC5460.10176428707220792No Hit
TATCCACATTGTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCT5450.10157790559405368No Hit
GTCTCACACCACTCAGAATGACTATTATTAAAAAGTCAAAAAATAACAGA5430.10120514263774524No Hit
CCTTTAGGTATGTACCCAATAATGGAATTGCTGGGTCAAATGGTAGTTAT5370.10008685376881987No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)