FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488717

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488717
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences536534
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAAATC27970.5213089943973728No Hit
GTACGGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGCAACTCTATG22850.4258816775824086No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA11780.21955738126567936No Hit
GGACTCTATCGTCCCCTTCTCCGTCTGCCGTTCCAGCCGACCACGGGGCG11660.21732080352782862No Hit
GTCCCGTACTGGTTGTTGTTGATCATTGAGAGTCCCGAGATTGAGATCTT10910.2033421926662616No Hit
GTATTAGGCTGTTCTTGCATTGCTATAAGGAAATACCTGAGACTGGGCAA10740.20017370753763974No Hit
TTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCTCGGGGC10640.19830989275609745No Hit
CTCCAAAAGAGACCAAATATTCAAGTACAGTTTCTCTTCCAAAAGTAAGG10590.19737798536532633No Hit
ATATGATCCTGTGGCAATGTTCCCCAACTTCCAATTATGTAACCCATGAA10030.18694062258868963No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATATCCACATTGTGTAAAT9560.17818069311544096No Hit
CCTCCACCTTATAGACCAGCAAATGCCCCTATCTTATCAACACTTCCGGA9240.1722164858145057No Hit
GTGTAAATGGAGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT8910.1660658970354162No Hit
GCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGCGCCCC8630.16084721564709784No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCACCTTATAGACCAGCAAATGCC7920.14761413069814774No Hit
ACTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTCCCTTCCGTCAG7690.1433273567006005No Hit
GTCCCGTACTGGTTGTTGTTGATCCTGGAATTAGAGGACAAACGGGCAAC7610.14183630487536672No Hit
GCCTTAGAGTCTCCTGAGCATTGCACACCTCACCATACTGCACTCAGGCA7450.13885420122489905No Hit
TCCTTGCACCATATAAAAATCAACTCAAAATGGATTAAAGACTTAAATGA7340.1368040049652026No Hit
CTCACCTGCCGGTCCGTGTGCACTTCGCTTCACCTCTGCACGTTGCATGG7070.13177170505503846No Hit
GTGTGAGACGGTATCTCATTGTGGTTTTGATTTGCATTTCTCTGATGGTT7050.13139894209873No Hit
ACCTGAGACTGGGCAATATTTAAAGAAAAGAGGTTTTTGGCTCAAGGCTC7050.13139894209873No Hit
ATAAAGCCCCAAGCGGCCCCGAGAGGGGTCGTCCGCGGGATTCAGCGCCG6860.12785769401379968No Hit
GTACAGGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG6660.12413006445071514No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6520.12152072375655597No Hit
GGATCATATTGTACAAAAGATCAAACACTGTTTTAGAAAACTTCCTGTTA6320.11779309419347142No Hit
TCAAAGACCACTGGCCAGCAGCCAACCAGGTAGGAGTGGGAGCATTCGGG6260.11667480532454608No Hit
CCTTTAGGTATGTACCCAATAATGGAATTGCTGGGTCAAATGGTAGTTAT6130.11425184610854111No Hit
CCCTAACAAAACAAAAAGATGGGGTTATTCCCTAAACTTCATGGGTTACA6110.11387908315223266No Hit
CCATTTACACAATGTGGATATCCTGCCTTAATGCCTTTGTATGCATGTAT6060.11294717576146153No Hit
CCTCCACCCTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA5760.10735573141683473No Hit
TTGTTCACACCTATTAATAATGTCCTCTTGTAAATGAATCTTAGGAAAGG5630.10493277220082976No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC5550.10344172037559596No Hit
ATACTATGCAGCCCTAAAAATTGAAATCATGCCTTTTGCAGCAACATGGA5530.10306895741928751No Hit
ATAGAGTCCCAAGCGGCCCCGAGAGGGGTCGTCCGCGGGATTCAGCGCCG5520.10288257594113327No Hit
GCCTAATACACTGCTTTCTGCAGTGGCTGAACTAATTTACATTCCCACCA5450.10157790559405368No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)