FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488718

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488718
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496915
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAAAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC27950.5624704426310334No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21620.43508447118722515No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20840.41938762162542886No Hit
CCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGGAC20190.40630691365726535No Hit
GCCGCCTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACC19840.39926345552056186No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16570.3334574323576467No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT16450.33104253242506265No Hit
CTTTCCGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGCT14250.2867693669943552No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC14180.2853606753670145No Hit
CCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGTC14080.28334825875652775No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC12290.2473260014288158No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC11870.23887385166477165No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11440.23022046023967882No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG11270.22679935200185142No Hit
ACCTCATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTG10780.21693851061046657No Hit
CATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATATACATCGT10500.21130374410110378No Hit
CCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAGCAAGCGGCTAGGAG10420.20969381081271443No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT10370.2086876025074711No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA10010.20144290270971899No Hit
CCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCATGGGCTATG9680.19480192789511286No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC9360.1883621947415554No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT9340.18795971141945805No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC9070.18252618657114394No Hit
GCCGGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC8940.17991004497751123No Hit
CAATTGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8870.17850135335017056No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8740.17588521175653785No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT8680.1746777617902458No Hit
GTCCAAGAGTCCTCTTATGTAAGAGAGTCCAAGAGTCCTCTTATGTAAGA8540.17186037853556443No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8520.17145789521346708No Hit
AGTCTAGACTCCGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8400.16904299528088307No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGG8370.16843927029773703No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC8040.1617982954831309No Hit
AGTTATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA7960.16018836219474156No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT7730.15555980399062214No Hit
GCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACG7710.1551573206685248No Hit
CATGTCCTACTGTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACC7510.1511324874475514No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA7490.15073000412545404No Hit
GTATCCATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAAT7400.148918829176016No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG7390.14871758751496736No Hit
GCACGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG7350.14791262087077267No Hit
CCTTTCCGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGC7310.14710765422657798No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG7040.14167412937826387No Hit
CTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGTCT6990.14066792107302054No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6930.13946047110672852No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGGACAAAGACC6780.13644184619099847No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT6600.1328194962921224No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG6590.13261825463107374No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6470.13020335469848968No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6430.12939838805429502No Hit
GATCCAACATCTAGGGACCTAGTTGTTAGTTATGTCAACACTAATATGGG6400.12879466307114898No Hit
CCTAGGACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGTC6210.12497107151122425No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG6150.12376362154493223No Hit
GGCCACTTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT6130.1233611382228349No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6090.12255617157864021No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6040.12154996327339686No Hit
TTGTCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC6010.12094623829025085No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC5980.12034251330710485No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC5970.12014127164605617No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC5960.11994002998500748No Hit
GGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAAC5920.11913506334081281No Hit
GATCAAGCATCTAGGGACCTAGTTGTTAGTTATGTCAACACTAATATGGG5900.11873258001871548No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5880.11833009669661813No Hit
CAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAG5830.11732388839137478No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC5820.11712264673032613No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5790.11651892174718011No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC5790.11651892174718011No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGG5780.11631768008613143No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5750.11571395510298543No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC5710.11490898845879073No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT5640.11350029683145003No Hit
GTGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5630.11329905517040138No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5550.11168912188201201No Hit
CCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTG5430.10927422194942799No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC5420.1090729802883793No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT5410.10887173862733063No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA5340.10746304699998993No Hit
TGGAAACCACCGTGAACGCCCACCAATTCTTGCCCAAGGTCTTACATAAG5340.10746304699998993No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCAGTACG5320.10706056367789259No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5300.10665808035579526No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC5270.10605435537264926No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT5170.10404193876216254No Hit
GTCTCCTGAGCATTGTTCACCTCACCATACTGCACTCAGGCAAGCAATTC5160.10384069710111386No Hit
TGCCTAGATTGAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACA5140.10343821377901653No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5120.10303573045691919No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5120.10303573045691919No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC5080.10223076381272451No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5070.10202952215167584No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG5050.10162703882957849No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5020.10102331384643248No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)