FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488719

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488719
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496915
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCAAAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC25960.522423352082348No Hit
GCCGCCTGGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACC25190.5069277441816005No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20160.40570318867411936No Hit
CCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGGAC19530.3930249640280531No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17260.3473431069700049No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT16280.3276214241872352No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA16050.32299286598311583No Hit
CTTTCCGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGCT15860.3191692744231911No Hit
CTCTATGGAAGGCGGGTATCTTATATAAGAGAGAAACAACACATAGCGCC11980.241087509936307No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT11590.23323908515540887No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG11120.22378072708612137No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC11070.222774518780878No Hit
CATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGCAAATATACATCGT11050.22237203545878068No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGC10850.21834720223780726No Hit
GCGTGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG10580.21291367738949318No Hit
CCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAATTTCATGGGCTATG10150.20426028596440038No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT10100.20325407765915698No Hit
CTCTTGGACTCTCTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAA9950.20023545274342694No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA9490.1909783363351881No Hit
CCGATAATGTTTGCTCCAGACCTGCTGCGAGCAAAGCAAGCGGCTAGGAG9000.18111749494380325No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT8670.17447652012919715No Hit
CAATTGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT8670.17447652012919715No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC8520.17145789521346708No Hit
GTCCCACGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC8500.17105541189136975No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC8100.16300574544942295No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT7970.1603896038557902No Hit
GCCGGAAAGGTTCCACGCATGCGCTGATGGCCCATGACCAAGCCCCAGCC7970.1603896038557902No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACAGGG7880.15857842890635218No Hit
CCTAGTACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCAGTC7750.15596228731271947No Hit
GCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACG7690.15475483734642748No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7620.15334614571908678No Hit
AGTCTAGACTCCGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7590.15274242073594074No Hit
CCTTTCCGGCTCCTCTGCCGATCCATACTGCGGAACTCCTAGCCGCTTGC7570.1523399374138434No Hit
CATGTCCTACTGTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACC7520.15133372910860005No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT7390.14871758751496736No Hit
GTATCCATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTAAAT7340.147711379209724No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7160.14408902931084794No Hit
GTCCAAGAGTCCTCTTATGTAAGAGAGTCCAAGAGTCCTCTTATGTAAGA7000.14086916273406921No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCTGTAATGTCAACGACCGACCT6890.13865550446253383No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAGACGGGCAAC6850.13785053781833914No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6730.1354356378857551No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6620.13322197961421975No Hit
GGCCACTTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT6540.13161204632583037No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6410.12899590473219766No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG6410.12899590473219766No Hit
GGCCTTTCTGTGTAAACAATATCTGAACCTTTACCCCGTTGCCCGGCAAC6400.12879466307114898No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC6380.12839217974905165No Hit
ACTAAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACAAATGG6350.12778845476590564No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG6340.12758721310485696No Hit
GTACAACATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6270.1261785214775163No Hit
AGTTATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA6260.1259772798164676No Hit
GATCAAGCATCTAGGGACCTAGTTGTTAGTTATGTCAACACTAATATGGG6120.12315989656178622No Hit
GCACGGGACCATGCAGAACCTGCACGACTACTGTTCAAGGAACCTCTATG6120.12315989656178622No Hit
ATACAAACCTTGCCAGCAAATCCGCCTCCTGCATCTACCAATCGCCAGTC6100.12275741323968888No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGATATTGTTTACAC6090.12255617157864021No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6080.12235492991759153No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG6040.12154996327339686No Hit
TGGAAACCACCGTGAACGCCCACCAATTCTTGCCCAAGGTCTTACATAAG6040.12154996327339686No Hit
GTGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG6000.12074499662920218No Hit
TTGTCAGGGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGC5990.1205437549681535No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGGACAAAGACC5950.11973878832395883No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5910.11893382167976416No Hit
GTTCAAGGAACCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGA5900.11873258001871548No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAAC5780.11631768008613143No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC5710.11490898845879073No Hit
CCACACTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTG5690.11450650513669339No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG5660.11390278015354738No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG5650.11370153849249871No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5620.1130978135093527No Hit
GATCCAACATCTAGGGACCTAGTTGTTAGTTATGTCAACACTAATATGGG5610.11289657184830404No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5590.11249408852620671No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA5560.11189036354306069No Hit
CCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAACCTAGCAGG5450.10967670527152532No Hit
CAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAG5450.10967670527152532No Hit
GGTTTAATACCCTTATCTAATGGCAAATATTTGGTAACCTTTGGATAAAA5380.10826801364418462No Hit
CTTTTTACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5300.10665808035579526No Hit
GGGCAAGAATTGGTGGGCGTTCACGGTGGTTTCCATGCGACGTGCAGAGG5270.10605435537264926No Hit
GTATAAAGGGACTCAAGGTGCTGTACAGACTTGGCCCCCAATACCACATC5220.1050481470674059No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5160.10384069710111386No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTACTGACTTCTTTCCTTCAGTACG5140.10343821377901653No Hit
CTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAA5090.10243200547377317No Hit
GTATGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC5000.10062083052433514No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)