FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488721

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488721
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences480837
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA27860.5794063268841624No Hit
ATTTACAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT24980.5195107697618944No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC23620.49122675667637894No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT21900.4554557989505799No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA21460.44630508883467784No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTT12800.2662024760989691No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12160.25289235229402063No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA12140.25247641092511597No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC11580.2408300525957861No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG10790.22440036852405285No Hit
GTATACATGCATATAAGGGCATTAATGCTGGGTAACCACATTGAGTAAAA10690.22232066167952963No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT10410.2164974825148647No Hit
CATCCAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT10380.21587357046150774No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC10220.21254603951027062No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG10010.20817865513677192No Hit
CCTGTAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT9430.19611635543853737No Hit
GTATGTAAATAATGTCTAGTTTGGAAGTAATGATTAACTGTATGTTCAGG9340.1942446192784665No Hit
GCATTAATGCCCTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTT9260.19258085380284795No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC9070.18862941079825388No Hit
GAGTAAAAGGGGCGGCAAATCCCAGAAGACCCACAATTCGTTGACATACT9040.18800549874489692No Hit
GCACTAAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTAC9010.18738158669153995No Hit
GTGTAAACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC8700.18093449547351806No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8510.17698305246892398No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8460.17594319904666236No Hit
CGCTCGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT8430.17531928699330543No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8160.1697040785130928No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAATCTCCTTTTTTCA7950.1653366941395941No Hit
GAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAAT7730.16076133908164306No Hit
GGGTAAAGGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7620.15847366155266754No Hit
GGATATTATCACCAGATGTCAACAATTTGTAGGCCCTCTTACAGTTAATG7530.15660192539259665No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7490.1557700426547874No Hit
CATCGAGCGATAACCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT7470.15535410128588273No Hit
GGCCCATGGCCAAGCCCCAACCAGTGGGGGTTGCGTCAGCAAACACTTGG7430.15452221854807346No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCGTCTATTCG7420.15431424786362113No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACA7390.15369033581026417No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA7340.1526504823880026No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC7300.15181859965019331No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC7290.15161062896574098No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG7280.15140265828128865No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG7260.15098671691238405No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG7170.14911498075231314No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT7160.14890701006786083No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7100.1476591859611469No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA7070.14703527390778998No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT7050.14661933253888532No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7030.14620339116998068No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6980.14516353774771906No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCGGGGCCG6830.14204397748093428No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6790.141212094743125No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG6790.141212094743125No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATCATT6650.13830050516079254No Hit
CATATAAGGGCATTAATGCTGGGTAACCACATTGAGTAAAAGGGGCGGCA6640.1380925344763402No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCCTCCCG6630.1378845637918879No Hit
GTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGG6590.1370526810540786No Hit
GTTGTCAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACTGCCGTG6560.13642876900072165No Hit
GTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATAC6300.13102153120496135No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG6290.13081356052050902No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6170.1283179123070812No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA6080.12644617614701031No Hit
CTGTAAGAGGGCCTACAAATTGTTGACATCTGGTGATAATATCCTCTTTT6060.12603023477810568No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6030.12540632272474872No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGATTATGCCTGCTAGG5940.12353458656467783No Hit
AGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAA5870.12207879177351161No Hit
CCCTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAAC5830.12124690903570232No Hit
GTTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG5800.12062299698234535No Hit
CCCCAAAAGACTGCCGTGTGGTGGAGTGAACCCTGGCCCGAATGATCCCA5730.11916720219117913No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACCGCTAT5710.1187512608222745No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5690.11833531945336985No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTCTGG5630.11708749534665593No Hit
GTACAAAGATCATTAACCTGATCTCCTCCCCCAACTCCTCCCAGTCTTTA5610.11667155397775128No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5590.11625561260884666No Hit
CCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGCTCCTCTGCCGATC5580.11604764192439433No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG5580.11604764192439433No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5520.11479981781768042No Hit
GTTCCGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG5510.1145918471332281No Hit
GCTTGTATACATGCATATAAGGGCATTAATGCTGGGTAACCACATTGAGT5490.11417590576432346No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5480.11396793507987114No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC5470.11375996439541881No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAA5450.1133440230265142No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5430.11292808165760955No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT5360.1114722868664433No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5350.11126431618199098No Hit
AGTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTTAAATGTATA5340.11105634549753868No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5340.11105634549753868No Hit
TTCGAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAG5330.11084837481308635No Hit
GTACAACATCTTGAATCCCTTTTTACCGCTATTACCAATTTTCTTTTGTC5330.11084837481308635No Hit
CCTCTCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5270.10960055070637242No Hit
CTTATATGCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTT5270.10960055070637242No Hit
CGCTGGATGTGTCTGCGGCGTTTTATCATCTTCCTCTTCATCCTGCTGCT5240.10897663865301548No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5170.10752084386184922No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5160.1073128731773969No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG5120.10648099043958764No Hit
CTTTTACTCAATGTGGTTACCCAGCATTAATGCCCTTATATGCATGTATA5100.106065049070683No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC5050.1050251956484214No Hit
TCCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTC5050.1050251956484214No Hit
TCCTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTG5050.1050251956484214No Hit
CCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAAGGGATTCAAGATG4990.10377737154170748No Hit
CATTAATGCTGGGTAACCACATTGAGTAAAAGGGGCGGCAAATCCCAGAA4990.10377737154170748No Hit
GAAGTTTCCGAAAACATTGTTTCAATTTTTGTACAATATGTTCCTGTGGC4970.10336143017280283No Hit
ATTTATGCCTACAGCCTCCCAGTACAAAGATCATTAACCTGATCTCCTCC4970.10336143017280283No Hit
TATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTC4950.1029454888038982No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4930.10252954743499357No Hit
GGTTGGGGCTTGGCCATGGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4910.10211360606608892No Hit
GGTTCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG4870.10128172332827963No Hit
GTCTTTGGGTATACATTTAAACCCTAATAAAACCAAACGTTGGGGTTACT4860.10107375264382733No Hit
TCATTAACCTGATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACACAG4810.10003389922156572No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)