FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488722

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488722
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517981
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA30360.5861218847795575No Hit
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACAGACT25700.4961571949550273No Hit
GGAACTGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG25280.4880487894343615No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT20710.39982161507854536No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA19110.3689324511902946No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG18330.35387398379477236No Hit
GTATAGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG17280.33360296999310785No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT16850.3253015071981405No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG16190.31255972709423707No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC16000.30889163888250726No Hit
GGAGTTGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG15970.3083124670596026No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA15460.29846654607022266No Hit
GCACAGTTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA15380.29692208787581015No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG15180.29306094238977876No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14220.27452744405682833No Hit
CCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT13090.25271197206075124No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT12800.24711331110600582No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC12620.24363828016857764No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG12140.23437153100210237No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11410.220278349978088No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG10870.20985325716580336No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10750.20753656987418456No Hit
ATATGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG10360.20000733617642347No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT10080.1946017324959796No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9940.19189893065575764No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9880.19074058700994825No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG9860.19035447246134513No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC9780.18881001426693259No Hit
GGTTAGGATAGAACCTAGCAGGCATAACCAATTTTAATCTCCTTTTTTCA9480.18301829603788555No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC9440.1822460669406793No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG8930.17240014595129938No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC8890.1716279168540931No Hit
CTACAAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA8790.16969734411107742No Hit
GTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTT8680.1675737140937602No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC8510.16429174043063355No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG8500.16409868315633197No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGGTTATGCCTGCTAGG8450.16313339678482414No Hit
CTACTGTACCTGTCTTTAATCCTCACTGGCAAACTCCCTCTTTTCCTCAC8310.1604305949446022No Hit
GCTCCGAATGCAGGGTCCAACTGGTGACCGGGAAAGAATCCCAGAGGATT8280.1598514231216975No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA8180.15792085037868184No Hit
CATTTAAATCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8120.15676250673287245No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT8020.15483193398985676No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA7990.15425276216695208No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7900.15251524669823796No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7900.15251524669823796No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7860.1517430176010317No Hit
CACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTG7800.1505846739552223No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7750.14961938758371446No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7750.14961938758371446No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7730.14923327303511133No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC7570.14614435664628625No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7540.14556518482338154No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC7540.14556518482338154No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG7500.1447929557261753No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA7450.14382766935466745No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7280.1405456956915408No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC7100.1370706647541126No Hit
GTACAAAGATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA7050.13610537838260478No Hit
CTCAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCAC6980.1347539774624938No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG6930.13378869109098596No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6810.13147200379936716No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6810.13147200379936716No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6770.1306997747021609No Hit
GTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTA6760.13050671742785933No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6760.13050671742785933No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT6740.1301206028792562No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6730.12992754560495462No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6720.12973448833065304No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGATTTAAATGTATA6690.12915531650774836No Hit
TCTCTATACAAAGCAGAGGCGGTATCGAGGAGATCACGAATAGAAGGAAA6600.12741780103903427No Hit
GATCATTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC6570.12683862921612954No Hit
GATTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA6530.12606640011892328No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6500.12548722829601858No Hit
GCTTTGTATAGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6440.12432888465020916No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6430.12413582737590761No Hit
GTGTAAACAATATATGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC6400.12355665555300291No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6240.12046773916417784No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6220.1200816246155747No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6200.11969551006697156No Hit
TGGTAAGTGATTGGAGGTTGGGGACTGCAAATTTTGGCCAGGACACGTGG6130.11834410914686061No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC6110.11795799459825747No Hit
AATATGGAGAGCACTACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6090.11757188004965433No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG6050.11679965095244807No Hit
CAATATATGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCA6020.11622047912954336No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG5900.11390379183792455No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5900.11390379183792455No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC5890.113710734563623No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC5880.11351767728932141No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5870.11332462001501986No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTGCTGACTTCTTTCCTTCTATTCG5870.11332462001501986No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA5860.1131315627407183No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC5850.11293850546641672No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5820.11235933364351203No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTGCTG5730.11062181817479792No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGCGTCCAAGGGAT5730.11062181817479792No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT5660.10927041725468695No Hit
CCATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATC5650.1090773599803854No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5650.1090773599803854No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5650.1090773599803854No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5620.10849818815748066No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5590.10791901633457597No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5570.10753290178597286No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCATG5540.10695372996306814No Hit
AGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCT5490.10598844359156032No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5480.10579538631725874No Hit
GGATAGAACCTAGCAGGCATAACCAATTTTAATCTCCTTTTTTCATTAAC5460.1054092717686556No Hit
ATACAGCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTG5440.10502315722005248No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5430.1048300999457509No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC5400.1042509281228462No Hit
CATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG5400.1042509281228462No Hit
GAATATGGAGAGCACTACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5350.10328564175133836No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5340.1030925844770368No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5320.10270646992843367No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA5300.10232035537983053No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAA5300.10232035537983053No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5290.10212729810552897No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5270.10174118355692582No Hit
TATTAACATTGACATAGCTTACTACTAATTCCCTGGATGCTGGGTCTTCC5260.10154812628262427No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGATTTAAATGTATAC5250.1013550690083227No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGC5210.10058283991111643No Hit
GCACGGGACCATGCAAGACCTGCACAGTTCCTGCTCAAGGAACCTCTATG5210.10058283991111643No Hit
ATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGTG5190.1001967253625133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)