FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488723

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488723
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences517981
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAGCCCAGAAGCCCCACAATTCGTTGACAGACT26140.5046517150242963No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA24030.4639166301466656No Hit
GGAACTGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG20400.3938368395751968No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG19240.371442195756215No Hit
CCTTTGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT18440.35599761381208966No Hit
GTCCCACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG18380.3548392701662802No Hit
GCACAACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA17920.34595863554840817No Hit
GTATAGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG17710.34190443278807525No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT17240.3328307408959016No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG16000.30889163888250726No Hit
GCACAGTTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA15210.2936401142126835No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGATC14160.273369100411019No Hit
CTACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA14030.27085935584509857No Hit
GGAGTTGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG13870.2677704394562735No Hit
CCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA12780.24672719655740272No Hit
CCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGAT12300.23746044739092745No Hit
ATATGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG12110.2337923591791977No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACT11900.22973815641886478No Hit
ATTCAGAGCCAATTCAAACAATCCAGATTGGGACTTCAACCCCAACAAGG11220.21661026176635823No Hit
GTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTA11100.21429357447473943No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11040.21313523082893004No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG10640.20541293985686734No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG10640.20541293985686734No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG10130.1955670188674874No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC9790.18900307154123414No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9440.1822460669406793No Hit
CTCCTACCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG9360.18070160874626676No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT9250.17857797872894954No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG9240.17838492145464793No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG9020.17413766142001347No Hit
GGTTAGGATAGAACCTAGCAGGCATAACCAATTTTAATCTCCTTTTTTCA8890.1716279168540931No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTC8710.16815288591666488No Hit
GTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAATCTT8640.16680148499655392No Hit
TCCTCAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG8550.16506396952783983No Hit
CTACAAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTA8430.16274728223622104No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTGGTTATGCCTGCTAGG8350.1612028240418085No Hit
GCTCCGAATGCAGGGTCCAACTGGTGACCGGGAAAGAATCCCAGAGGATT8280.1598514231216975No Hit
CATTTAAATCCTACTAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8150.15734167855577713No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG8120.15676250673287245No Hit
GGGTAAAGGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8070.1557972203613646No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC8040.1552180485384599No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG7840.15135690305242855No Hit
GGTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7840.15135690305242855No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7590.14653047119488938No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7550.14575824209768312No Hit
GTGTAAACAATATATGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTC7530.14537212754908No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATG7500.1447929557261753No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7380.1424762684345565No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7340.1417040393373502No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC7320.14131792478874708No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG7300.14093181024014395No Hit
CTACTGTACCTGTCTTTAATCCTCACTGGCAAACTCCCTCTTTTCCTCAC7260.14015958114293767No Hit
CACTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTG7240.13977346659433454No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7150.13803595112562042No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA7120.13745677930271574No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT7060.13629843565690633No Hit
TCTCTATACAAAGCAGAGGCGGTATCGAGGAGATCACGAATAGAAGGAAA7050.13610537838260478No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC7050.13610537838260478No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA7040.1359123211083032No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA7010.1353331492853985No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6770.1306997747021609No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6760.13050671742785933No Hit
GCTTTGTATAGAGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA6700.1293483737820499No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG6660.12857614468484366No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6600.12741780103903427No Hit
CTCAATTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCAC6600.12741780103903427No Hit
CAATATATGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCA6580.12703168649043112No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6530.12606640011892328No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6420.12394277010160605No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6390.12336359827870136No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC6370.12297748373009822No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6370.12297748373009822No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6370.12297748373009822No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGATTTAAATGTATA6340.12239831190719351No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT6290.12143302553568568No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6230.12027468188987628No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6220.1200816246155747No Hit
GTTCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6210.11988856734127314No Hit
GGATAGAACCTAGCAGGCATAACCAATTTTAATCTCCTTTTTTCATTAAC6140.11853716642116216No Hit
AATATGGAGAGCACTACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6120.11815105187255902No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC6120.11815105187255902No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6050.11679965095244807No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATA6050.11679965095244807No Hit
ATATATGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAG6000.11583436458094024No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6000.11583436458094024No Hit
GCGGTAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGAAC5990.11564130730663866No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5920.1142899063865277No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCC5900.11390379183792455No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAGCAGGCTTTCACTTT5860.1131315627407183No Hit
GTTCAGGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTA5790.11178016182060732No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5700.1100426463518932No Hit
CTCCTACCTGGTGTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC5690.10984958907759165No Hit
CCCCAAAAGATTGCCGTGTGGTGGAGTGAACCCTGGCCCGAATGATCCCA5670.10946347452898851No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5650.1090773599803854No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5640.1088843027060838No Hit
GATTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAA5630.10869124543178225No Hit
ATATAAGAGAGAAACTACTCGCAGCGCCTCATTTTGTGGGTCACCATATT5590.10791901633457597No Hit
CATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAG5540.10695372996306814No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5540.10695372996306814No Hit
TGGTAAGTGATTGGAGGTTGGGGACTGCAAATTTTGGCCAGGACACGTGG5480.10579538631725874No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5480.10579538631725874No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT5470.10560232904295717No Hit
CTTTTGGAGTGTGGATTCGCACTCCTACCGCTTACAGACCACCAAATGCC5470.10560232904295717No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5460.1054092717686556No Hit
GTTTGGGACTCTACCGTCCCCTTCTTCGTCTGCCGTTCCGACCGACCACG5410.10444398539714778No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCCCATATCGTCAATCTTCTCG5400.1042509281228462No Hit
GACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATACAC5400.1042509281228462No Hit
GATAATATCCTCTTTCAAATGAATGTGAGGAAAAGAGGGAGTTTGCCAGT5380.10386481357424306No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5360.10347869902563994No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTAGTAGGATTTAAATGTATAC5360.10347869902563994No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGTTATCGCTGGATGTGTC5350.10328564175133836No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGCGTCCAAGGGAT5350.10328564175133836No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAGTATAGGCCCCT5340.1030925844770368No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTGCTG5290.10212729810552897No Hit
GAATATGGAGAGCACTACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5250.1013550690083227No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACTCGCAGCGCCTCAT5230.10096895445971957No Hit
GCACGGGACCATGCAAGACCTGCACAGTTCCTGCTCAAGGAACCTCTATG5210.10058283991111643No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)