FastQCFastQC Report
Thu 1 Sep 2022
EGAF00002488724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences151266
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7280.481271402694591No Hit
GCATTCGACAAGGCATGGGGACGAATCTTTCTGTTCCCAATCCTCTGGGA5960.39400790660161567No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC5920.3913635582351619No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT4680.3093887588750942No Hit
GTCGAATGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGA4560.3014557137757328No Hit
GTCGAGGTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGA4470.2955059299512118No Hit
CCTCCTGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4280.28294527521055624No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC3840.2538574431795645No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA3670.24261896262213584No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG3670.24261896262213584No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG3590.23733026588922826No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT3480.2300583078814803No Hit
GTGTAAAAGGGGCAGCAAATCCCAAAAGACCCACAATTCTTTGACATACT3360.22212526278211892No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA3340.220803088598892No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3340.220803088598892No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3090.20427591130855577No Hit
CTTATATAGAATGCCAGCCTTCCACAGAACATGTAAATAATGCCTAGTTT3080.20361482421694235No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG2970.1963428662091944No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG2930.19369851784274061No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT2790.18444329856015232No Hit
CTATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATA2770.18312112437692543No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT2770.18312112437692543No Hit
ATTTTGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA2660.17584916636917747No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA2610.17254373091111025No Hit
CAGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT2570.16989938254465645No Hit
GGGTTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAT2540.1679161212698161No Hit
ATCTCAATGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGG2510.16593285999497576No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2490.16461068581174884No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAA2450.16196633744529504No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT2350.15535546652916057No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG2280.1507278568878664No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT2270.15006676979625297No Hit
CTCTTACTGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG2250.14874459561302608No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGATGA2190.14477807306334536No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2180.14411698597173192No Hit
CCTCCTGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA2160.142794811788505No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG2150.14213372469689156No Hit
ATATAAGAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATATT2080.13750611505559743No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACTGTTAATGAAAAA2060.1361839408723705No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA2010.1328785054143033No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG1970.1302341570478495No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT1920.12692872158978225No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA1910.12626763449816877No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC1900.12560654740655533No Hit
CATGTATACAAGCTAAGCAGGCTTTCACGTTCTCATCAACTTACAAGGCC1890.1249454603149419No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT1890.1249454603149419No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC1880.12428437322332844No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAACATGTAAATAATGCCTAGT1870.12362328613171499No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA1870.12362328613171499No Hit
ATATGTTCTTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA1860.12296219904010156No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG1860.12296219904010156No Hit
CTCCAAAAGACACCAAATACTCAAGAACGGTTTCTCTTCCAAAAGTAAGA1830.12097893776526121No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC1830.12097893776526121No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG1820.12031785067364775No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA1810.1196567635820343No Hit
GTTTCCCTCTTATATAGAATGCCAGCCTTCCACAGAACATGTAAATAATG1810.1196567635820343No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTC1800.11899567649042084No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA1770.11701241521558049No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA1760.11635132812396706No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT1760.11635132812396706No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC1740.11502915394074015No Hit
GACTTTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGC1730.11436806684912672No Hit
GGGTTCACCCCTCCACACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA1730.11436806684912672No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC1720.11370697975751326No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG1690.11172371848267292No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT1630.10775719593299221No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA1630.10775719593299221No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA1620.10709610884137877No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG1620.10709610884137877No Hit
GTAGAAGAGTAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT1620.10709610884137877No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG1610.10643502174976531No Hit
ATTTGGTGGTCTGTAAGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA1600.10577393465815187No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC1580.10445176047492497No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG1580.10445176047492497No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTAATCTCCTCC1560.10312958629169808No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC1550.10246849920008462No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG1540.10180741210847118No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG1530.10114632501685772No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA1520.10048523792524428No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)