FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488726

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488726
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences383773
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC17280.4502661729720434No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14660.3819966490607729No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA11730.30564943338900863No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG11630.3030437263694945No Hit
ATATAGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC11530.3004380193499803No Hit
GATTTGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA10780.2808952167036243No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC10390.2707329593275191No Hit
TGGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTAC10250.26708496950019933No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10050.261873555461171No Hit
TCTTATTGGTTCTTCTGGACTACCAAGGTATGTTGCCCGTTTGTCCTCTA9920.2584861363358027No Hit
CCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGAATATGATAAAA9420.24545760123823196No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8930.2326896368426127No Hit
AACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGAC8700.22669651069773017No Hit
GTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGCAGTGCTTCAT8700.22669651069773017No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA7920.2063719959455199No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7910.20611142524356846No Hit
CCTTTCTCCAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGG7700.20063944050258878No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT7320.19073775382843502No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7270.18943490031867796No Hit
CAGTAACCCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG7200.18761090540501807No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC6940.1808360671542813No Hit
CTCTCAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGATTG6760.17614579451915585No Hit
GTCCCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCTGACTGCCGAT6670.17380065820159313No Hit
CTCACTGTTAGTATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCT6510.1696315269703705No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6490.16911038556646768No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6380.16624410784500213No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6360.1657229664410993No Hit
ATATAAAAGAGAAACTACACGCAGTGCTTCATTTTGTGGGTCACCATATT6270.16337783012353657No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6230.1623355473157309No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6230.1623355473157309No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6180.16103269380597385No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG6100.15894812819036253No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG6090.15868755748841112No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6060.1579058453825569No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6020.15686356257475123No Hit
GCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG5970.15556070906499414No Hit
GATTTTTAGGCCCATATTAACATTGACATAGCTGACTACTAATTCCCTGG5680.14800415870840314No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAGCAAACAGTCTTTGA5630.14670130519864608No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG5580.14539845168888899No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5510.1435744567752291No Hit
GTCAACGAATTGTGGGTCTTTTGGGCTTTGCTGCCCCTTTTACACAATGT5460.14227160326547203No Hit
CTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCTTG5440.1417504618615692No Hit
GGAAGATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT5330.13888418414010364No Hit
GCAGACGCAGAAGGGGACGATAGAGGCCCAAACGGCCCCGAGACGGGTCG5290.13784190133229798No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT5240.13653904782254092No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5210.1357573357166867No Hit
ATACCACATCATCCATATAACTGAAGGCCAAACAGTGGGGGAAAGCCCTG5180.13497562361083243No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4950.12898249746594995No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA4900.12767964395619286No Hit
ATTTACATACCCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA4890.12741907325424146No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4880.12715850255229003No Hit
CGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTACGTCC4880.12715850255229003No Hit
GGATAAAGGCATTAAACCTTATTATCCTGAACATGCAGTTAATCATTACT4750.12377108342692164No Hit
GCCTAGTCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4740.12351051272497024No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4740.12351051272497024No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG4730.12324994202301881No Hit
GCCTCCCACAGGGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAAC4720.12298937132106741No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4690.12220765921521316No Hit
CTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTG4690.12220765921521316No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4670.12168651781131035No Hit
CTGCATCTGCCGTTCCGGCCGACCACGGGGCGCACCTCTCTTTACGCGGT4650.12116537640740752No Hit
CTGGCTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTG4630.12064423500350469No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4600.11986252289765043No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4560.11882024008984479No Hit
CCTAAAACTCAGACGACTATTGTGGTTTCACATTTCCTGTCTTACTTTTG4540.11829909868594196No Hit
GGCCCACTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAA4500.1172568158781363No Hit
TCTGCGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4470.11647510377228204No Hit
GCACACCCGAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAAC4430.11543282096447641No Hit
TTTCTGTTCCCAATCCTCTGGGATTCTTTCCCGATCACCAGTTGGACCCT4430.11543282096447641No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4390.11439053815667075No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4370.11386939675276792No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGAGAAAGGCATTAAACCTTAT4360.11360882605081649No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAGATATAGGCCCCT4270.11126368973325376No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA4270.11126368973325376No Hit
CAGCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATC4260.11100311903130236No Hit
ATACTATATCATCCATATAACTGAAGGCCAAACAGTGGGGGAAAGCCCTG4260.11100311903130236No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCGCG4220.1099608362234967No Hit
CATTTGAACCCTAATAAAACCAAACGCTGGGGCTACTCCCTTAACTTCAT4180.10891855341569104No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTTACAGGTAGTTTCCG4170.10865798271373964No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4160.10839741201178821No Hit
GATTGGCCTCTGGCCAGTGATCCTTGTTGGGGTTGAAGTCCCAATCTGGA4150.1081368413098368No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCTGGTCTCTGCCAAGTGTTTGCTG4100.10683398780007973No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4080.10631284639617691No Hit
ACATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACC4050.10553113429032267No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4020.10474942218446842No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4000.10422828078056559No Hit
ATTTACAGGTAGTTTCCGAAAACATTGCTTGAGTTTTAAGACAATATGTT4000.10422828078056559No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG4000.10422828078056559No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3980.10370713937666277No Hit
GCCACACAGTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGT3970.10344656867471134No Hit
GCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC3940.10266485656885711No Hit
TTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC3930.1024042858669057No Hit
GGATTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCCATTTG3920.10214371516495428No Hit
GGTTTGCTGTAGCTCTTGTTCCCAAGAATATGGTGACCCACAAAATGAAG3860.10058029095324579No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3840.10005914954934299No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)