FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488728

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488728
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378064
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14300.37824283719158663No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12710.33618646578357103No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12590.3330124000169284No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACGCTTCCGGA12160.3216386643531254No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG12040.3184645985864827No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11100.29360108341444835No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA10220.27032460112573536No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9890.2615959202674679No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9760.2581573490202717No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA9630.25471877777307544No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT9620.25445427229252193No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC9470.25048669008421853No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA9140.24175800922595114No Hit
GTGTAAAAGGAGCAGCGAAGCCTAAAAGACCCACAATCCTTTGACATACT8610.22773921875661263No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8600.2274747132760591No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8230.21768801049557746No Hit
ATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT8160.21583647213170254No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8000.21160438444284566No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGCAGAGGACAAACGGGCAAC7350.19441152820686444No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG7270.192295484362436No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTTT7250.19176647340132885No Hit
CCCTTAACTTCATGGGATACGTAATTGGAAGTTGGGGTACTTTACCACAG7200.1904439459985611No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA7190.19017944051800753No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7000.18515383638748995No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAAC6990.1848893309069364No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6990.1848893309069364No Hit
CTATTAATAATGTCCTCCTTTAAATGAATGTTAGGAAAAGAGGGAGTTTG6960.18409581446527573No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6940.1835668035041686No Hit
GGGTAAAGGTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6890.18224427610140082No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA6820.18039273773752593No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6810.18012823225697236No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC6400.1692835075542765No Hit
GGATATTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA6180.16346438698209828No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6090.16108383765711626No Hit
GGGTTCACCCCACCTCACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA6020.15923229929324134No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6020.15923229929324134No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5970.15790977189047356No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACGTATCCCATGAA5920.15658724448770578No Hit
CCTTTAAATGAATGTTAGGAAAAGAGGGAGTTTGCCACTCAGGATTAAAG5820.15394218968217022No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5770.15261966227940243No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG5710.1510326293960811No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5670.14997460747386684No Hit
GTTATTAGACAACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG5670.14997460747386684No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT5660.1497101019933133No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5630.14891658555165263No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACAAAATGCC5580.14759405814888485No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5440.14389098142113504No Hit
GTGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA5430.1436264759405815No Hit
GTCTAATAACAGTAGTTTCCGGAAGCGTTGATAAGATAGGGGCATTTTGT5340.14124592661559948No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5290.1399233992128317No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA5200.13754284988784968No Hit
GTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA5160.13648482796563544No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5050.1335752676795463No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5030.1330462567184392No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4990.13198823479622498No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4980.13172372931567142No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTAAAGG4940.1306657073934572No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4940.1306657073934572No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4940.1306657073934572No Hit
TCATTAACCTGGTCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG4930.13040120191290364No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4900.12960768547124296No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4880.12907867451013585No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGAGCAGCG4880.12907867451013585No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4810.12722713614626097No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4790.12669812518515386No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4790.12669812518515386No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4780.1264336197046003No Hit
CCCCAAAAGACCGCCGTGAGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4750.1256401032629396No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC4670.12352405941851116No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4580.12114351009352914No Hit
CAATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCA4570.12087900461297557No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTC4550.12034999365186846No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4540.1200854881713149No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCGAGCTGTGC4460.11796944432688646No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA4460.11796944432688646No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4460.11796944432688646No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACGGAGTTGTCAGTC4430.11717592788522578No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4390.11611790596301155No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4390.11611790596301155No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4380.11585340048245799No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4380.11585340048245799No Hit
AAACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAA4330.11453087307969022No Hit
ATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAG4330.11453087307969022No Hit
ATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG4320.11426636759913665No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGGCCCTTTTTACCTCTAT4310.11400186211858308No Hit
TCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGA4290.11347285115747598No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4280.11320834567692244No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT4250.11241482923526175No Hit
GGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTTGGTAAGGTT4240.11215032375470818No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4230.11188581827415464No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4230.11188581827415464No Hit
CCTTATGAGTCCAAGGGATATTAACATTGAGATCCCCGAGATTGAGATCT4230.11188581827415464No Hit
ATTTATGCCTACAGCCTCCTAGTACAAAGATCATTAACCTGGTCTCCTCC4220.11162131279360107No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT4180.11056329087138686No Hit
GCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4170.1102987853908333No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4170.1102987853908333No Hit
GTGTAAACAATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4150.10976977442972617No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4140.10950526894917263No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4140.10950526894917263No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCA4130.10924076346861906No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4130.10924076346861906No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4120.10897625798806553No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTGCTTCCAGGAACATCAACTACC4120.10897625798806553No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGGCCCTTTTTACC4090.10818274154640484No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4060.10738922510474416No Hit
GGATAGCCACATTGTGTAAAAGGAGCAGCGAAGCCTAAAAGACCCACAAT4060.10738922510474416No Hit
ATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTTCCGGA4050.10712471962419062No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT4040.10686021414363706No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4020.10633120318252993No Hit
GGTAAAAAGGGCCTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4010.10606669770197638No Hit
CATACTGCGGAACTCCTAGCGGCTTGTTTTGCTCGCAGCCGGTCTGGAGC3970.10500867577976215No Hit
TTGTACACCTCACCATACAGCACTCAGGCAAGCTATTCTGTGTTGGGGTG3970.10500867577976215No Hit
GTGGGGGAAAGCCCTGCGAACCACTGAACAAATGGCACTAGTAAACTGAG3950.10447966481865505No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG3920.10368614837699436No Hit
GTCCAAGGGATATTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGAC3910.10342164289644082No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC3910.10342164289644082No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3900.10315713741588725No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC3880.10262812645478014No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG3850.10183461001311946No Hit
CTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATATTGACAACAGTGCCAGC3800.10051208261035169No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3790.10024757712979812No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACACAC3790.10024757712979812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)