FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488729

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488729
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378064
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC14580.38564899064708624No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG14110.373217233061069No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG12210.3229611917558932No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACGCTTCCGGA12070.3192581150281434No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11160.2951881162977697No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11010.29122053408946635No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT10200.26979559016462823No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT9950.26318295315078927No Hit
CTATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATA9860.26080240382580727No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA9130.2414935037453976No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT8950.23673240509543358No Hit
GTGTAAAAGGAGCAGCGAAGCCTAAAAGACCCACAATCCTTTGACATACT8910.23567438317321937No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG8770.23197130644546957No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8610.22773921875661263No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA8190.21662998857336324No Hit
ATATAAGAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT7780.20578526387066742No Hit
CCCTTAACTTCATGGGATACGTAATTGGAAGTTGGGGTACTTTACCACAG7480.19785009945406068No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7270.192295484362436No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7260.19203097888188242No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTTT7130.1885924076346862No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7090.18753438571247194No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG7010.1854183418680435No Hit
CTATTAATAATGTCCTCCTTTAAATGAATGTTAGGAAAAGAGGGAGTTTG6910.18277328706250792No Hit
ATGTAAGACCTTGGGCAAGACCTGGCGGGCGTTCACGGTGGTCTCCATGC6880.18197977062084728No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC6740.17827669389309747No Hit
GGGTAAAGGTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6700.17721867197088323No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGCAGAGGACAAACGGGCAAC6520.1724575733209192No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG6440.17034152947649076No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA6400.1692835075542765No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACGTATCCCATGAA6300.16663845274874095No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6230.16478691438486606No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6110.16161284861822336No Hit
GGATATTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGACGCGGCGA6090.16108383765711626No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6000.15870328833213423No Hit
CCTTTAAATGAATGTTAGGAAAAGAGGGAGTTTGCCACTCAGGATTAAAG5960.15764526640992002No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5920.15658724448770578No Hit
GTTAATATCCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTC5840.15447120064327732No Hit
GGGTTCACCCCACCTCACGGCGGTCTTTTGGGGTGGAGCCCTCAGGCTCA5830.15420669516272376No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA5790.15314867324050954No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5700.15076812391552752No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAAC5610.14838757459054552No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5440.14389098142113504No Hit
CCTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTAT5380.1423039485378137No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG5380.1423039485378137No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT5330.14098142113504591No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG5300.14018790469338524No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5280.13965889373227813No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5180.13701383892674257No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACAC5170.136749333446189No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACAAAATGCC5120.13542680604342122No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5100.13489779508231411No Hit
GTCTAATAACAGTAGTTTCCGGAAGCGTTGATAAGATAGGGGCATTTTGT5040.13331076219899277No Hit
ATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAG5040.13331076219899277No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATG4980.13172372931567142No Hit
GTTATTAGACAACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCG4960.1311947183545643No Hit
CCTTATGAGTCCAAGGGATATTAACATTGAGATCCCCGAGATTGAGATCT4950.13093021287401077No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4920.13013669643235007No Hit
GTGCATATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA4900.12960768547124296No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGAGCAGCG4810.12722713614626097No Hit
CAATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCA4690.12405307037961827No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCTTTTCCTAACATTCATTTAAAGG4660.1232595539379576No Hit
CCCCAAAAGACCGCCGTGAGGTGGGGTGAACCCTGGCCCGAATGCTCCCG4650.12299504845740404No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4640.12273054297685047No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4610.1219370265351898No Hit
GTGTGTAGTTTCTCTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTA4560.12061449913242203No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGGCCCTTTTTACC4530.11982098269076136No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTGCTTCCAGGAACATCAACTACC4500.11902746624910068No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC4480.11849845528799356No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4430.11717592788522578No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG4410.11664691692411866No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4410.11664691692411866No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG4340.11479537856024377No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA4330.11453087307969022No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4290.11347285115747598No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGACTTTATTCTTCTACTG4290.11347285115747598No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4270.11294384019636887No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA4260.11267933471581532No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGGCCCTTTTTACCTCTAT4220.11162131279360107No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4210.11135680731304753No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4210.11135680731304753No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT4200.11109230183249397No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGTCCAGTAAAGTTTCCCAC4190.11082779635194041No Hit
GTCCAAGGGATATTAACATTGAGATCCCCGAGATTGAGATCTTCTGCGAC4190.11082779635194041No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA4180.11056329087138686No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT4170.1102987853908333No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4160.11003427991027974No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG4160.11003427991027974No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4140.10950526894917263No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG4100.1084472470269584No Hit
GTGTAAACAATATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC4050.10712471962419062No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3990.10553768674086926No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG3980.10527318126031572No Hit
GGATAGCCACATTGTGTAAAAGGAGCAGCGAAGCCTAAAAGACCCACAAT3980.10527318126031572No Hit
TTATTATCCTGAACATGCAGTTAATCATTACTTCAAAACTAGGCATTATT3950.10447966481865505No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC3930.10395065385754793No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT3930.10395065385754793No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA3930.10395065385754793No Hit
ATATGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG3930.10395065385754793No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT3920.10368614837699436No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3880.10262812645478014No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC3830.10130559905201235No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTCG3810.10077658809090524No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCACTCAGGCA3790.10024757712979812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)