FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488732

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488732
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549681
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG22860.4158775726284881No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC19250.3502031178083288No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA18530.3371046115838095No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN17000.309270285856706No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC15100.27470478331978No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT14520.26415320886113947No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14320.2605147349098841No Hit
GAACAAGTAGTTAATCATTACTTCAAAACTAGGCATTATTTACATACTCT13860.25214624482199677No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC13860.25214624482199677No Hit
CTTTAGTGGTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGAC13480.24523314431461157No Hit
GGGGCTGTGGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTAG13310.24214044145604452No Hit
ACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTATATTTTCC12680.23067924850959012No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12530.22795039304614856No Hit
GAAGATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACATACTCT12050.2192180555631357No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11960.21758074228507082No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA11900.2164892000996942No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11210.20393646496786316No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA11000.20011606731904505No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11000.20011606731904505No Hit
GCACCAAAGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTC10980.19975221992391953No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCAAAG10570.192293348323846No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT10470.19047411134821832No Hit
GCATCTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTT10260.1866537136994002No Hit
GGGGCTGTGGTGGAGTTCCACTGTATGGCCTGAGGATGACTGTCTCTTAG10070.1831971634457076No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG10050.18283331605058206No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT9710.17664791033344796No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9340.16991673352362552No Hit
CTGCATCTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT8960.16300363301624032No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8790.15991093015767327No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC8470.1540893718356647No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA8420.15317975334785083No Hit
ATTTACATACTCTGTGGAAGGCCGGCATTCTATATAAGAGAGAAACTACA8320.15136051637222317No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC8280.1506328215819721No Hit
GTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT7870.14317394998189859No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7830.14244625519164753No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7810.14208240779652198No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7720.14044509451845705No Hit
CTACCTGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT7710.1402631708208943No Hit
GGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG7550.13735239165989No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7470.13589700207938787No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7450.13553315468426233No Hit
CTTATATAGAATGCCGGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7300.1328042992208208No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7210.1311669859427559No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7110.12934774896712822No Hit
CCTCCAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7050.1282562067817516No Hit
CTACTTGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT7010.1275285119915005No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6990.127164664596375No Hit
CCACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTATATTTT6930.12607312241099838No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6880.12516350392318454No Hit
CTATTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG6860.12479965652805901No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6860.12479965652805901No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6790.12352619064511963No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6760.12298041955243133No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6700.12188887736705471No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA6540.11897809820605042No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA6440.11715886123042274No Hit
AAGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATACTCAAGA6190.11261076879135352No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6110.11115537921085139No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6100.1109734555132886No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6100.1109734555132886No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCATGT6030.10969998963034923No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6010.10933614223522371No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5940.10806267635228431No Hit
GGGTTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5920.1076988289571588No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5870.10678921046934495No Hit
CCTTTACCCCGTTGCCAGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG5770.10496997349371726No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5760.1047880497961545No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5740.10442420240102895No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC5680.10333266021565234No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5660.10296881282052682No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5600.1018772706351502No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5500.10005803365952252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)