FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488733

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488733
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences549681
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATCAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG22970.4178787333016786No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA18360.3340119087252425No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC17570.3196399366177838No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16950.30836066736889217No Hit
CTTTAGTGGTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGAC14380.2616062770952607No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14190.2581497268415681No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13460.24486929691948603No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC13050.23741042531941256No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12660.23031540111446455No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA12310.2239480716997677No Hit
TAGCCACACTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCT11990.2181265133777591No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG11740.2135784209386899No Hit
ACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTATATTTTCC11480.2088484048020579No Hit
GGGGCTGTGGTGGAGTTCCACTGCATGGCCTGAGGATGACTGTCTCTTAG11320.20593762564105364No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGATCTTCGTCTGCGAGGCGA11090.20175338059710995No Hit
CTATTAATAATGTCCTCATGTAAATGAATGTTAGGAAAGGAGGGAGTTTG11010.2002979910166078No Hit
GCACCAAAGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTC10590.19265719571897155No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCAAAG10210.18574409521158636No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT10140.18447062932864697No Hit
GAACAAGTAGTTAATCATTACTTCAAAACTAGGCATTATTTACATACTCT10040.1826513923530193No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG9990.18174177386520546No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT9920.18046830798226607No Hit
GCATCTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATATTT9520.17319136007975536No Hit
ATCTCAATCTCGGGAATCTCAATGTTAGTATCCCTTGGACTCATAAGGTG9510.1730094363821926No Hit
GAAGATGCAGTTAATCATTACTTCAAAACTAGGCATTATTTACATACTCT9060.16482286999186802No Hit
TTATTGATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA8750.15918323536742218No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8750.15918323536742218No Hit
GTACTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT8310.1511785926746604No Hit
GGGGCTGTGGTGGAGTTCCACTGTATGGCCTGAGGATGACTGTCTCTTAG8300.15099666897709763No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8070.14681242393315394No Hit
CTGCATCTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT8000.14553895805021458No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7890.14353779737702413No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7590.13808008645014108No Hit
TATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAAA7450.13553315468426233No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG7440.13535123098669957No Hit
TCCCATGGCTGCTCGGATGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7430.1351693072891368No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG7410.13480545989401127No Hit
ATTTACATACTCTGTGGAAGGCCGGCATTCTATATAAGAGAGAAACTACA7410.13480545989401127No Hit
CTACCTGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT7310.13298622291838358No Hit
GGTTAGGATAAAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7180.13062121485006759No Hit
CTTATATAGAATGCCGGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT7140.12989352005981653No Hit
CTATTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG7080.12880197787443992No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6950.12643696980612393No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6890.12534542762074732No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA6850.12461773283049624No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6790.12352619064511963No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6720.12225272476218026No Hit
CCACATTCCACCAAGCTCTGCTAGATCCCAGAGTGAGGGGCCTATATTTT6620.12043348778655255No Hit
GGTGCTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTG6610.12025156408898979No Hit
CCTTTACCCCGTTGCCAGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG6580.11970579299630149No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6540.11897809820605042No Hit
CCTCCAGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6520.11861425081092487No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG6410.11661309013773444No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATCAATAATGTCCTCATGT6330.11515770055723228No Hit
CTACTTGTTCAGGATAATAAGGTTTAATGCCTTTATCCAAGGGCAAATAT6270.11406615837185569No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6250.11370231097673014No Hit
GGAATCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG6100.1109734555132886No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5950.1082446000498471No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAA5930.10788075265472155No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5820.10587959198153112No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCAGGCAACGGTCCGGTC5760.1047880497961545No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG5760.1047880497961545No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC5710.10387843130834065No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5700.1036965076107779No Hit
GCCTCACTTTTGGAAGAGAAACTGTTCTTGAGTATTTGGTGTCTTTTGGA5650.10278688912296403No Hit
GTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGG5610.10205919433271297No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5590.10169534693758744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)