FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488736

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488736
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences449054
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA22870.5092928690090724No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC16890.3761240296267264No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACACTGCTGAGAGTCCAA15200.33848935762736776No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA13210.2941739746222058No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC12440.2770268163739773No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12170.2710141764687543No Hit
GACATCTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT12120.26990072463445375No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT11870.264333465462951No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC10990.2447367131792613No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10700.23827869254031808No Hit
ATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAGATCCCAGAGT10240.22803493566475302No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG10070.22424919942813115No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC9340.20799280264734307No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG9210.20509782787816164No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG9200.20487513751130154No Hit
CCACCGTATGGCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAG9120.20309361457642064No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGAATTAAACCTTAT8290.1846103141270315No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCGTG8180.18216072009157028No Hit
AAAACTCAGACATCTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAA8060.17948843568924897No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA8040.17904305495552872No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7890.1757026994526271No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT7860.17503462835204675No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7800.17369848615088607No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCGCCAATCGGCAGT7760.17280772468344566No Hit
ATAGTTGTCTGATTTTTAGGCCCATATTAACATTGACATAGCTGACTACT7520.167463155878803No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG7480.16657239441136257No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7380.16434549074276145No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT7370.16412280037590135No Hit
TTATTAGGGTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGA7340.163454729275321No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7300.16256396780788057No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGTCGTTC7220.1607824448729997No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTACTAATAATGTCCTCCTGT7100.15811016047067836No Hit
CTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGAGTCTCCGGA7020.15632863753579748No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7020.15632863753579748No Hit
TACCAGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGT6970.15521518570149692No Hit
ATGTAAGAGCTTGGGCAAGACCTGGTGGTCGTTCACGGTGGTCTCCATGC6700.14920254579627396No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6600.14697564212767283No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6550.14586219029337227No Hit
GTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6470.1440806673584914No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6400.14252183479047065No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC6370.1418537636898903No Hit
GTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGAAACATAGAGGTTC6260.1394041696544291No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA6230.13873609855384877No Hit
CTATTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG6150.13695457561896787No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6140.13673188525210775No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC6090.13561843341780722No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC6040.13450498158350666No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6020.13405960084978644No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6000.13361422011606622No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5970.13294614901548588No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5920.13183269718118534No Hit
GCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTGGCCAC5910.13161000681432522No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5850.13027386461316456No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5830.12982848387944435No Hit
TGGTTGACTTCTCTCAATTTTCTAGGGGGAGCACCCACGTGTCCTGGCCA5820.12960579351258425No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5820.12960579351258425No Hit
TACCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGT5780.12871503204514378No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5770.1284923416782837No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5760.12826965131142357No Hit
CTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTG5720.12737888984398313No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5670.12626543800968257No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA5660.12604274764282247No Hit
GACAACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACT5640.12559736690910225No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5630.12537467654224213No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC5590.1244839150748017No Hit
CATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCAGGTCTGGAGC5580.12426122470794158No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA5570.12403853434108146No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5510.1227023921399208No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5500.12247970177306071No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC5470.12181163067248038No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5440.12114355957190004No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT5320.11847127516957871No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5320.11847127516957871No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG5310.1182485848027186No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5300.11802589443585851No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5190.11557630040039726No Hit
GACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGG5170.11513091966667706No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5080.11312670636493606No Hit
GAATTAGTAGTCAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACA5070.11290401599807595No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC5040.11223594489749562No Hit
CCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCATGGGATATG5030.11201325453063553No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCTGGAACGG5020.11179056416377539No Hit
ATACAGCACTCAGGCAAGCTATTTTGTGTTGGGGTGAGTTGATGAATCTG4980.11089980269633497No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT4950.11023173159575464No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA4780.10644599535913274No Hit
TCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAACAG4780.10644599535913274No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4770.10622330499227264No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT4770.10622330499227264No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG4760.10600061462541253No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG4750.10577792425855241No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4750.10577792425855241No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCGAGCAGCC4730.1053325435248322No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4720.10510985315797208No Hit
GTCGTTGACACTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGAGCTTGGG4690.10444178205739175No Hit
GTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG4690.10444178205739175No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4680.10421909169053166No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4680.10421909169053166No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC4560.10154680728821032No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4530.10087873618762999No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4530.10087873618762999No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4530.10087873618762999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)