FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488738

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488738
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences568687
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN19250.3384990337391219No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18590.32689335258235197No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17830.31352923488667755No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA16410.28855943603423323No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG15380.2704475396835166No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC15070.26499638641291257No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14750.25936938948841803No Hit
GTTGACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGCTTA14550.255852516410609No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG14210.2498738321783336No Hit
CTGTATCAACAATACCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGG13640.2398507439065778No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC13290.23369621602041193No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACAGACT12530.2203320983247375No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG12030.21153991563021488No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT11920.20960563543741986No Hit
GACTTCTTTCCTTCTATTCGAGATCTCCTTGACACCGCCTCTGCTCTGTA11910.20942979178352944No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC11240.19764826697286908No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG10960.1927246446639364No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT10960.1927246446639364No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG10590.18621842946998965No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA10410.18305324369996148No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCTTCCTGT10350.18199818177661878No Hit
ACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA10340.1818223381227283No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG10030.17637118485212427No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT9960.1751402792748911No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG9840.17303015542820568No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC9320.1638862854259021No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG9320.1638862854259021No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA9180.16142447427143577No Hit
GGGGCTACTCCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACT8850.15562163369305082No Hit
GAGTATCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATACCCAAAGA8770.1542148844619272No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG8740.15368735350025586No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG8710.15315982253858448No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG8670.15245644792302268No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8610.15140138599967998No Hit
ACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCCAGGTGGC8340.14665360734463773No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC8160.1434884215746096No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA8010.1408507667662528No Hit
GTATTAAAACTCAAGCAATGTTTTCGGAAACTGCCTGTAAATAGACCTAT8000.14067492311236235No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7950.13979570484291007No Hit
GCATGGGAGGTTGGTCTTCCAAACCTCGACAAGGCATGGGGACGAATCTT7940.13961986118901962No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC7800.13715805003455328No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT7440.13082767849449697No Hit
CTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG7370.1295967729172638No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7360.12942092926337337No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC7340.12906924195559244No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA7310.1285417109939211No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT7290.12819002368614016No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCCTTAAACAAAC7280.12801418003224974No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7260.12766249272446883No Hit
GGTTAGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCAGGACACG7220.126959118108907No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA7180.1262557434933452No Hit
AACTTGTGTTATTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT7110.12502483791611202No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGATAG7100.12484899426222157No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC7070.1243214633005502No Hit
GCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG6800.11957368464550798No Hit
CTATTAATAATGTCTTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6800.11957368464550798No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6750.11869446637605573No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC6730.11834277906827481No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6680.11746356079882256No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6600.11605681156769893No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6590.11588096791380847No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6570.11552928060602756No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA6560.11535343695213711No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6520.1146500623365753No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC6490.11412253137490394No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC6480.11394668772101349No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG6390.11236409483599942No Hit
AACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT6360.11183656387432805No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT6340.11148487656654714No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6330.11130903291265669No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA6230.10955059637375217No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6160.108319690796519No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT6110.10744047252706672No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA6090.10708878521928583No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT6090.10708878521928583No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT6080.10691294156539538No Hit
ACACAAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA6010.10568203598816221No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5950.1046269740648195No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA5940.10445113041092904No Hit
ATATAGGCCCCTCACTCTGGGATCTATCAGAGCTTGGTGGAATGTTGTGG5910.10392359944925768No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC5900.10374775579536721No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5900.10374775579536721No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT5880.10339606848758633No Hit
GTTTGGAAGACCAACCTCCCATGCTGTAGCTCTTGTTCCCAAGAATATGG5870.10322022483369588No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG5860.10304438117980541No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5840.10269269387202451No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG5770.10146178829479133No Hit
AAATAGACCTATTGATTGGAAAGTCTGTCAAAGAATTGTGGGTCTTTTGG5730.10075841367922954No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)