FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488740

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488740
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401516
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA15290.38080674244612917No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13860.3451917233684336No Hit
GTGTAAAGGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12600.3138106576076669No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11180.2784446946074378No Hit
TAGCCACACTGTGTAAAGGGGGCAGCAAAGCCCAAAAGACCCACAATTCT7870.19600713296605865No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7790.19401468434632743No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT7690.1915241235716634No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7430.18504866555753693No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC7350.18305621693780572No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA7140.17782603931101126No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6760.16836190836728798No Hit
CCTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6450.1606411699658295No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCG6450.1606411699658295No Hit
CCCTTAACTTCATGGGGTATGTAATTGGAAGTTGGGGTACTTTACCACAA6260.15590910449396786No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6230.15516193626156866No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA6160.15341854371930386No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5940.14793931001504299No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC5720.14246007631078214No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5650.14071668376851731No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5550.13822612299385328No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5530.1377280108389205No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5530.1377280108389205No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5370.13374311359945804No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA5180.1290110481275964No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5110.1272676555853316No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5070.126271431275466No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4980.12402992657826836No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4950.12328275834586916No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4760.1185506928740075No Hit
CTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAA4700.11705635640920911No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4680.1165582442542763No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4660.11606013209934347No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA4570.11381862740214588No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG4560.11356957132467947No Hit
CCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTT4420.11008278624014983No Hit
GACCACTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC4320.10759222546548582No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG4320.10759222546548582No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4310.10734316938801941No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACACTGTGTAAAG4210.10485260861335538No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4190.10435449645842258No Hit
TCCTAACATTCATTTACAGGAGGACATTATTAATAGATGTCAACAATATG4190.10435449645842258No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4140.10310921607109057No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4130.10286015999362416No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC4120.10261110391615776No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)