FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488741

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488741
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences401516
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18460.45975751900297873No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14590.363372817023481No Hit
GTGTAAAGGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT12660.31530499407246537No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11140.2774484702975722No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGGCCG9150.22788631088175815No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG7840.19525996473365942No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG7520.18729017025473454No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6910.17209774952928403No Hit
TAGCCACACTGTGTAAAGGGGGCAGCAAAGCCCAAAAGACCCACAATTCT6830.1701053009095528No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6620.16487512328275836No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6450.1606411699658295No Hit
CCTTTACACAGTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA6410.1596449456559639No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA6350.15815060919116547No Hit
GTGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATACAAGCTAAGC5830.14519969316291256No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5760.14345630062064774No Hit
CCCTTAACTTCATGGGGTATGTAATTGGAAGTTGGGGTACTTTACCACAA5690.14171290807838294No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5670.14121479592345013No Hit
GGTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5600.1394714033811853No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG5470.13623367437412207No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5330.13274688928959244No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5280.13150160890226045No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT5230.13025632851492844No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5230.13025632851492844No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT5180.1290110481275964No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG5010.12477709481066757No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4960.12353181442333555No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC4940.12303370226840275No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG4840.12054314149373872No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCCGGTCTCTGCCAAGTGTTTGCTG4780.11904880502894033No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA4700.11705635640920911No Hit
ATTGACAACAGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT4680.1165582442542763No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT4540.11307145916974665No Hit
CTTTATACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4500.11207523485988105No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4430.11033184231761624No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4380.10908656193028422No Hit
GACCACTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCC4220.10510166469082179No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4190.10435449645842258No Hit
CTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAA4180.10410544038095618No Hit
CCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTT4130.10286015999362416No Hit
GGATTAAAGATAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4060.10111676745135935No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)