FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488743

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488743
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences258328
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14420.5582050726208541No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10350.4006534328450652No Hit
GTGTAAAAGGGGCAACAAATCCCAAAAGACCCACAATTCTTTGACACACT6200.2400049549410052No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA5910.22877891672602274No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA5890.22800470719395496No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGCTGGGGTACTTTACCACAG4970.19239106871883807No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4960.1920039639528042No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG4940.19122975442073645No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA4900.18968133535660092No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGCTT4750.18387476386609272No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA4750.18387476386609272No Hit
GTATGTAAATAATGCCTAGCTTGGAAGTAATGATTAACTGCATGTTCAGG4740.18348765910005885No Hit
GGATAGCCACATTGTGTAAAAGGGGCAACAAATCCCAAAAGACCCACAAT4430.17148741135300857No Hit
ATATGTTCCTGTGGTAAAGTACCCCAGCTTCCAATTACATATCCCATGAA4420.1711003065869747No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC4310.16684215416060202No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4150.16064847790405998No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4040.15639032547768728No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG4000.15484190641355178No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC3990.15445480164751788No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC3930.1521321730513146No Hit
CATTTGAACCCTCATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT3910.15135796351924685No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT3890.15058375398717908No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA3690.1428416586665015No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGTCAACTTACAAGGCC3640.1409061348363321No Hit
CTATTAATAATGTCCTCCTGCAAATGAATATTAGGAAAGGAGGGAGTTTG3630.14051903007029823No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG3620.14013192530426435No Hit
GTCTCGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCG3620.14013192530426435No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA3560.13780929670806108No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC3530.13664798240995943No Hit
GGCTTTCACTTTCTCGTCAACTTACAAGGCCTTTCTGTGTAAACAATATC3500.13548666811185778No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT3460.13393824904772228No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAACA3350.1296800966213496No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGACGA3350.1296800966213496No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC3330.12890588708928183No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3270.12658325849307855No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3250.1258090489610108No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC3240.12542194419497693No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG3200.1238735251308414No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG3190.12348642036480754No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGAACGTCC3160.1223251060667059No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG3150.12193800130067202No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG3110.12038958223653651No Hit
GGAACATATTGTACAAAAACTTAAGCAATGTTTTCGGAAACTGCCTGTAA3090.11961537270446874No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTAATAATGTCCTCCTGC3090.11961537270446874No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG3050.11806695364033322No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC3040.11767984887429934No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3010.1165185345761977No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATGAGGGTTCAAATGTATA2890.11187327738379114No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC2880.11148617261775727No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACATTCCACCAAGCTCTGCTAG2850.11032485831965563No Hit
CTCTAAGGCCTCCCGATAGAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA2840.10993775355362174No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT2800.10838933448948623No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG2760.10684091542535071No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC2730.10567960112724908No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG2720.10529249636121521No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATCATT2710.10490539159518132No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2710.10490539159518132No Hit
ATGTAATTGGAAGCTGGGGTACTTTACCACAGGAACATATTGTACAAAAA2700.10451828682914745No Hit
GCTTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGT2690.10413118206311356No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA2630.10180855346691028No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGC2620.10142144870087641No Hit
GTGATTCGCAGGGCTTTCCCCCACTGTTTGGCTTTCAGTTATATGGATGA2600.10064723916880865No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)