FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488744

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488744
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192217
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN11810.6144097556407602No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4870.25335948433281136No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG4480.23306991577227823No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT4440.23098893438145432No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT4420.22994844368604234No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC4360.22682697159980644No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAAATC4360.22682697159980644No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGATGTTGACATTGCTGAGAGTCCAA4230.22006378207962873No Hit
ATGTAAGACCTTGGGCAAGACCTGATGGGCGTTCACGGTGGTCTCCATGC4040.2101791204732152No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG3660.190409797260388No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGATTTCTTTCCTTCTATTCG3460.18000489030626843No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACTTTACCACAG3380.1758429275246206No Hit
CATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC3370.17532268217691463No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG3370.17532268217691463No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT3350.17428219148150267No Hit
ATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCAA3240.16855949265673692No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3220.16751900196132496No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTGCCAATTACATATCCCATGAA3110.1617963031365592No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC2950.15347237757326354No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT2930.15243188687785159No Hit
CCCTAGCAGCCATGGGAATGAGGTGTATTTCCGAGAGAGGACAACCGAGT2920.15191164153014564No Hit
ATCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAATTTTACTGGG2850.14826992409620376No Hit
CCTTTATATTCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAAC2770.14410796131455594No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2750.143067470619144No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG2730.14202697992373203No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC2700.1404662438806141No Hit
CTCTTACGGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG2690.13994599853290812No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACGGTTAATGAAAAA2650.1378650171420842No Hit
TCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAG2640.1373447717943782No Hit
CATCCAGCGATAGCCAGGACAAATTGGAGGACAAGAGGTTGGTGAGTGAT2600.13526379040355432No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG2550.13266256366502444No Hit
GTATACATGAATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA2520.1311018276219065No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGATGGGCGTTC2470.1285006008833766No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT2450.12746011018796463No Hit
GTACAAAAATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTA2450.12746011018796463No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2430.12641961949255268No Hit
TCCGCGAGAGGACAACCGAGTTGTCAGTCCCGATGAGTTTCGCTCCAGAC2400.12485888344943474No Hit
GTCCCGTGCTGGTAGTTGACGTTCCTGGAAGTAGAGGACAAACGGGCAAC2370.12329814740631682No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC2270.11809569392925703No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2260.11757544858155107No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC2230.11601471253843312No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG2230.11601471253843312No Hit
GGGTTAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAGCCAC2200.1144539764953152No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC2190.11393373114760921No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA2190.11393373114760921No Hit
TGTCTGCGGCGTTTTATCATATTCCTCTTCATCCTGCTGCTATGCCTCAT2180.11341348579990322No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG2170.11289324045219726No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG2160.11237299510449127No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACATCCGACCT2150.1118527497567853No Hit
ATATTCATGTATACAAGCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA2110.10977176836596139No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA2090.10873127767054944No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATTCATGTATA2090.10873127767054944No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA2080.10821103232284346No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA2080.10821103232284346No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGATCT2070.10769078697513747No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC2060.1071705416274315No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG2060.1071705416274315No Hit
CCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGAAGTATGCCTCA2060.1071705416274315No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC2050.10665029627972551No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTTTATATAAGAGAGAAACTACA2050.10665029627972551No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2050.10665029627972551No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA2040.10613005093201955No Hit
GCCTCACCCATATCGTCAATCTTCTCGAGGACTGGGGACCCTGCACCGAA2040.10613005093201955No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG2030.10560980558431356No Hit
GTTTCGCTCCAGACCGGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCA2000.10404906954119564No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG1980.10300857884578368No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG1970.10248833349807769No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)