FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488749

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488749
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences410937
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17960.4370499614296109No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17760.4321830353557845No Hit
ATTAACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT12290.29907260723663237No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12250.2980992220218671No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12000.2920155644295841No Hit
GTTGTTAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG11880.2890954087852882No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG10580.25746038930541665No Hit
GTATACATGCATATAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA10520.25600031148326874No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9730.23677595349165442No Hit
GTATGTAAATAATGTCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG9510.23142233481044538No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCGCAG9310.226555408736619No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATA9120.22193182896648392No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9070.2207150974480273No Hit
CATGTATACAATCTAAGCAAGCTTTCACTTTCTCGCCAACTTACAAGGCC8850.21536147876681827No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGTTT8780.213658054640979No Hit
ATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAACCTTAT8380.2039242024933262No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTTGTTGACATTGCTGAGAGTCCAA7790.18956677057553833No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTGTGGGTCACCATATT7770.18908007796815568No Hit
CCCTAGCAGCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7550.18372645928694664No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG7320.1781294943020463No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7280.17715610908728102No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6900.16790894954701086No Hit
ATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC6850.16669221802855425No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG6840.16644887172486295No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6780.164988793902715No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6610.16085190673996258No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6520.1586617900067407No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6510.1584184437030494No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6000.14600778221479205No Hit
CTATTAATAATGTCCTCCTGTAAATGAATATTAGGAAAGGAGGGAGTTTG6000.14600778221479205No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5980.1455210896074094No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5960.14503439700002677No Hit
GTAGAGGCCCAAGCGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG5920.14406101178526148No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAT5790.14089750983727434No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5760.14016747092620035No Hit
GGCCTCTACCGTCCCCTTCTTCAGCTGCCGTTCCGGCCGACCACGGGGCG5620.13676062267452188No Hit
CTCTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGTCTAGT5580.1357872374597566No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT5540.13481385224499132No Hit
GGTTCGCCCCAGACCGGCTGCGAGCAAAACACGCTGCTAGGAGTTCCGCA5510.13408381333391736No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5370.1306769650822389No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5350.13019027247485623No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGCCCCGTG5350.13019027247485623No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5300.12897354095639965No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA5250.12775680943794304No Hit
ATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAA5220.12702677052686906No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5150.12532334640102982No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5070.12337657597149929No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5060.12313322966780796No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5050.12288988336411664No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGCGGTAAAAA5030.122403190756734No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4990.12142980554196872No Hit
ATCTAAGCAAGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTA4980.1211864592382774No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4940.12021307402351213No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG4930.1199697277198208No Hit
GGATGGGGCTTGGCCGTAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC4890.11899634250505552No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4890.11899634250505552No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4830.1175362646829076No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG4820.11729291837921628No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTATATGCATGTATA4770.11607618686075968No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG4710.11461610903861176No Hit
GCTAGGAGTTCCGCAGTATGGATCGGCAGAGGAGCCACAAAGGTTCCACG4620.11242599230538988No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4610.11218264600169854No Hit
GTTCTATCCTAACCTTACCAAATATTTACCCTTGGATAAAGGCATTAAAC4590.11169595339431593No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4430.10780241253525479No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4400.10707237362418084No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4390.1068290273204895No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAATATTCATTTACAGG4370.10634233471310686No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4360.10609898840941556No Hit
GTCTTACATAAGAGGACTCTTGGACTCTCAGCAATGTCAACAACCGACCT4340.10561229580203291No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC4280.10415221797988498No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT4230.10293548646142839No Hit
GCCTTAATGCCTTTATATGCATGTATACAATCTAAGCAAGCTTTCACTTT4220.10269214015773709No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGGTGGGCGTTC4170.10147540863928046No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4140.10074536972820651No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4130.10050202342451518No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)