FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488751

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488751
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences378312
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC12940.34204571887754026No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG12120.32037048785129735No Hit
CCTCCCGCTTACAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA11290.29843092473936855No Hit
GTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAA10690.28257099959821524No Hit
ATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCATGC10660.2817780033411576No Hit
GTATGTAAATAATGCCTAGTTTTAAAGTAATGATTAACTGCATGTTCCGG10420.27543403328469623No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9800.2590454439721711No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTTTGACATACT9420.24900082471610735No Hit
GGTATAAAGGGCCTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT9350.2471505001163061No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT9340.24688616803062022No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA9070.23974920171710123No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC8220.21728097443380065No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCCATGAA7750.2048573664065639No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA7600.20089238512127558No Hit
GGATAGCCACATTGTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAAT7460.1971917359216731No Hit
GCTAGATCCCAGAGTGAGGGGCCTATATTTTCCTGCTGGTGGCTCCAGTT7400.19560574340755776No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG7340.19401975089344245No Hit
ATATAAAAGAGAAACTACACACAGCGCTTCATTTTGTGGGTCACCATATT7270.19216942629364123No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTACGCGGGACGTCC7250.19164076212226946No Hit
GTTGTCAATGTGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG7080.18714711666560935No Hit
ACTACACACAGCGCTTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA7010.1852967920658081No Hit
GCACGGGACCATGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATG6970.18423946372306457No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATATCCTCCTGT6850.1810674786948339No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6830.18053881452346213No Hit
GGATCTAGCAGAGCTTGGTGGAATGTTGTGGAGTTCCACTGCATGGCCTG6750.17842415783797502No Hit
CTTTATACCTCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA6490.1715515236101419No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG6450.17049419526739834No Hit
GACTTCTTTCCTTCTGTTCGAGATCTCCTTGACACCGCCTCTGCTCTGTA6400.16917253483896888No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA6250.16520755355368058No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6200.16388589312525112No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC6160.16282856478250757No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC6130.1620355685254499No Hit
GGTAAAAAGGGCCTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT6080.16071390809702046No Hit
ACGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGAT6030.159392247668591No Hit
CATATAAAGGCATCAAAGCAGGATAGCCACATTGTGTAAAAGGGGCAGCA5940.15701325889741802No Hit
CCCATCTCTCCACCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAA5800.15331260969781557No Hit
GAGATATGGGAGTAGGCTGTCTTCCTGACTGCCGATTGGTGGAGGCAGGA5690.1504049567552708No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATATC5670.149876292583899No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA5570.1472329717270401No Hit
GTATAAAGGGCCTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC5560.1469686396413542No Hit
GTGTAAACAATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTC5530.14617564338429656No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5520.14591131129861068No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACATTCCACCAAGCTCTGCTAG5490.145118315041553No Hit
GGGTAAAGGTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5420.14326799044175179No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCAAGAAGATTGACGATATGG5420.14326799044175179No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5370.14194633001332232No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5300.14009600541352113No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACAAAATGCC5280.13956734124214934No Hit
GGGTCGAGTTGATGAATCTGGCCACCTGGGTGGGAAGTAATTTGGAAGAT5270.13930300915646346No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5040.133223371185688No Hit
ACTTTTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTC5030.13295903910000212No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5010.13243037492863033No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAATAAACAGTCTTTGA5000.13216604284294445No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT4870.12872972572902788No Hit
GGATGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC4860.128465393643342No Hit
CTGCCTCGTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGG4840.1279367294719702No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4740.12529340861511135No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT4730.12502907652942544No Hit
ATGTTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4710.12450041235805367No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4710.12450041235805367No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC4700.12423608027236778No Hit
GTTTTAAAGTAATGATTAACTGCATGTTCCGGATAATAAGGTTTAATGCC4690.1239717481866819No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC4660.12317875192962423No Hit
CCCATATCTCCACCTCTAAGAGACAGTCATCCTCAGGCCATGCAGTGGAA4660.12317875192962423No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4650.12291441984393833No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG4650.12291441984393833No Hit
GGAACATATTGTACAAAAACTAAAGCAATGTTTTCGGAAACTGCCTGTAA4610.12185709150119477No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACAT4580.12106409524413712No Hit
CTTATATAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTTTCCA4580.12106409524413712No Hit
CCACAATAGTTGTCTGATTTTTAGGCCCATATTAACATTGACATAGCTGA4560.12053543107276533No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACTTTACCACAG4560.12053543107276533No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4550.12027109898707945No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4540.12000676690139356No Hit
CTTTAAAACTAGGCATTATTTACATACTCTGTGGAAGGCTGGCATTCTAT4500.11894943855864999No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC4430.11709911395884878No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTCTTGCATGGTCCCGTG4420.11683478187316289No Hit
CTTTTACACAATGTGGCTATCCTGCTTTGATGCCTTTATATGCATGTATA4300.11366279684493223No Hit
TATTAACATTGACATAGCTGACTACCAATTCCCTGGATGCTGGATCTTCC4300.11366279684493223No Hit
GTGTGTAGTTTCTCTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTA4290.11339846475924634No Hit
GTCTTATATAAGAGGACTCTTGGACTCTCAGCAATGTCAACGACCGACCT4290.11339846475924634No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4260.11260546850218867No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT4230.111812472245131No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4210.11128380807375923No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4190.11075514390238746No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTTAGTTTTTGTACAATA4190.11075514390238746No Hit
ACTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTC4180.11049081181670156No Hit
CCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGACA4130.10916915138827211No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGATATGGGAGTAGGCTGTCT4110.10864048721690032No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC4100.10837615513121446No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4100.10837615513121446No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC4100.10837615513121446No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG4090.10811182304552855No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4070.10758315887415679No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC4050.10705449470278501No Hit
GTACAATATGTTCCTGTGGTAAAGTACCCCAACTTCCAATTACATATCCC4020.10626149844572734No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3950.1044111738459261No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3950.1044111738459261No Hit
GATTGTATACATGCATATAAAGGCATCAAAGCAGGATAGCCACATTGTGT3940.10414684176024024No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA3910.10335384550318256No Hit
TGCAAGACCTGCACGACTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG3900.10308951341749667No Hit
CGGTGGTTTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG3890.10282518133181077No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT3890.10282518133181077No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG3890.10282518133181077No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCACCCATATCGTCAATCTTCTTG3830.10123918881769543No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC3820.10097485673200957No Hit
CCCCAAAAGACCGCCGTGAGGTGGGGTGAACCCTGGCCCGAATGCTCCCA3820.10097485673200957No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA3810.10071052464632367No Hit
GTCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCGACGGG3810.10071052464632367No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)