FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488753

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488753
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences309269
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16870.5454798250067094No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG10980.3550307337625174No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG10720.3466238129266108No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG10570.34177366629051087No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGCGTAAAA9830.31784627621908434No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC9330.3016791207654178No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTAAGAGTCCAA7980.2580278010405181No Hit
CATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC7680.2483275077683182No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACA7600.24574076289573155No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC7540.24380070424129155No Hit
CTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG7540.24380070424129155No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG7200.23280703853279833No Hit
ATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAACCTTAT6870.22213671593337836No Hit
CTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTCG6740.21793325551542506No Hit
ATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATATT6720.2172865692972784No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTG6550.2117897364430318No Hit
GCTTAGATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT6540.21146639333395845No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG6510.21049636400673846No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC6380.20629290358878516No Hit
ATATGTTCCTGTGGTAACGTACCCCAACTTCCAATTACATATCCCATGAA6280.20305947249805184No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTAATAATGTCCTCCTGT6230.2014427569526852No Hit
ATTCAAGATCTCCTCGACACCGCTTCTGCTCTGTATCGGGAGGCCTTAGA6120.19788598275287855No Hit
ATATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA6030.19497589477121857No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA5970.1930358361167786No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG5870.18980240502604528No Hit
GGGTAAAGGTGCAGGTATTGTTGACACAGAAAGGCCTTGTAAGTTGGCGA5720.18495225838994533No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC5680.18365888595365198No Hit
CTCACCAACCTCTTGTCCTCCAATTTGTCCTGGCTATCGCTGGATGTGTC5660.18301219973550534No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACGTTACCACAG5540.17913208242662537No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC5390.17428193579052542No Hit
TTTACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACAC5370.17363524957237875No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5230.1691084460453521No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5200.1681384167181321No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5120.16555167184554548No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5070.1639349563001788No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGCGTAAAAGGGGCAGCA5070.1639349563001788No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5060.16361161319110548No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAATGAAAAA4820.15585137857334555No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4780.1545580061370522No Hit
CTTTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGT4750.15358797680983222No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4720.15261794748261223No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4650.1503545457190989No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4650.1503545457190989No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC4620.1493845163918789No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG4600.14873783017373227No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4570.14776780084651225No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG4540.14679777151929227No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGTTACTCCCTTAACTTCAT4530.14647442841021893No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG4460.14421102664670563No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTG4330.14000756622875232No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC4320.13968422311967899No Hit
CTACTGTACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAAC4300.1390375369015323No Hit
GCGTAAAAGGGGCAGCAAAGCCCAAAAGACCTACAATTCTTTGACATACT4230.136774135138019No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG4220.13645079202894567No Hit
ATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTC4210.13612744891987233No Hit
CTCCATGTTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG4210.13612744891987233No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4200.13580410581079902No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4120.13321736093821235No Hit
GTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC4110.13289401782913904No Hit
GGTTAGTGAGTGATTGGAGGTTGGGGACTGCGAATTTTGGCCAGGACACG4070.13160064539284572No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG4060.13127730228377238No Hit
CAACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTG4060.13127730228377238No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC4030.1303072729565524No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTATTACATAACCT4010.12966058673840572No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG4010.12966058673840572No Hit
GGGTTTACTGTTCCGGAACTGGAGCCACCAGCAGGAAAATATAGGCCCCT3960.1280438711930391No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG3930.12707384186581908No Hit
GGATGGGGCTTGGCCATAGGCCATCGGCGCATGCGTGGAACCTTTGTGGC3900.1261038125385991No Hit
AACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT3880.12545712632045242No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA3810.1231937245569391No Hit
GGATTAAAGACAGGTACAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC3800.12287038144786577No Hit
AACTTGTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCT3740.12093032279342579No Hit
TACATACTCTGTGGAAGGCTGGCATTCTATATAAAAGAGAAACTACACGC3690.11931360724805914No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC3640.11769689170269247No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA3590.11608017615732581No Hit
ATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTTGTGG3590.11608017615732581No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC3570.11543348993917917No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC3560.11511014683010584No Hit
GTCTATTTACAGGCAGTTTCCGAAAACATTGCTTGATTTTTAATACAATA3550.11478680372103249No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTC3540.11446346061195917No Hit
ACAGTAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTGTA3530.11414011750288584No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTGGATAAAGGCATTAAAC3500.11317008817566584No Hit
GATTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGCGT3490.11284674506659251No Hit
CCTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAAC3470.11220005884844586No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC3470.11220005884844586No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTAACATTCATTTACAGG3440.11123002952122586No Hit
CTATATAAAAGAGAAACTACACGCAGCGCTTCATTTTGTGGGTCACCATA3430.11090668641215254No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG3410.11026000019400586No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG3360.1086432846486392No Hit
GGAAAATATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGT3340.10799659843049256No Hit
ATTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT3320.10734991221234588No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT3320.10734991221234588No Hit
GGAACATATTGTATTAAAAATCAAGCAATGTTTTCGGAAACTGCCTGTAA3290.1063798828851259No Hit
CTATGGGAGTGGGCCTCAGTCCGTTTCTCCTGGCTCAGTTTACTAGTGCC3280.10605653977605256No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3250.10508651044883258No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3230.1044398242306859No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG3230.1044398242306859No Hit
ACTTATCGGGACTGACAACTCTGTTGTCCTCTCTCGGAAATACACCTCCT3220.10411648112161258No Hit
CAGTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTG3220.10411648112161258No Hit
GTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAAGGGAT3220.10411648112161258No Hit
ATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC3220.10411648112161258No Hit
ACTACTGTTGTTAGACGACGAGGCAGGTCCCCTAGAAGAAGAACTCCCTC3220.10411648112161258No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT3210.10379313801253925No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG3210.10379313801253925No Hit
GGATAGCCACATTGCGTAAAAGGGGCAGCAAAGCCCAAAAGACCTACAAT3190.10314645179439258No Hit
GTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTAT3190.10314645179439258No Hit
CTTTATATGCATGTATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACT3180.10282310868531926No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3160.1021764224671726No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG3150.10185307935809924No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC3150.10185307935809924No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT3140.10152973624902592No Hit
CAACACAAGTTTCCGGAAGTGTTGATAAGATAGGGGCATTTGGTGGTCTG3130.1012063931399526No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG3130.1012063931399526No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA3100.1002363638127326No Hit
AGTTAAGGGAGTAACCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA3100.1002363638127326No Hit
CCTGTAAATGAATGTTAGGAAAGGAGGGAGTTTGCCACTCAGGATTAAAG3100.1002363638127326No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)