FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002488754

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488754
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489145
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAG22290.4556930971388852No Hit
CTCCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT18440.37698432979995705No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16730.3420253708000695No Hit
GTGCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA16650.34038986394627363No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA16130.32975906939660016No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG15200.31074630222122274No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG12490.2553435075488863No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11760.24041950750799865No Hit
ATATTTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAG11550.23612630201678436No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACTTTACCACAG11260.2301975896717742No Hit
GGCCCATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG10900.22283780882969262No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10560.21588690470106No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC10430.21322920606364165No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC10330.21118482249639675No Hit
CTATAGGCCATCGGCGCCTGAGTGGAACCTTTGTGGCTCCTCTACCGATC10050.20546054850811107No Hit
GTCTATTAACAGGCAATTTCCGAAAACAATGCTTGATTTTTTGTACAATA9900.20239397315724378No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTGCCAATTACATATCCCATGAA9610.1964652608122336No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC9600.19626082245550908No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATCTCCTCCCC9490.19401200053153972No Hit
GTGTAAAAGGGGCAGCGAAGCCCAAAAGACCCACAATTCTTTGACATACT9290.18992323339704995No Hit
GGGATACCGCGTAAAGAGAGGTGCGCCCCGTGGTTGGCCGGAACGGCAGA9260.1893099183268765No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC8950.18297232926841733No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8900.18195013748479488No Hit
GTATGGATCGGTAGAGGAGCCACAAAGGTTCCACTCAGGCGCCGATGGCC8850.18092794570117246No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8730.1784746854204786No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8700.17786137035030514No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG8600.17581698678306024No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8380.1713193429351215No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG8320.17009271279477456No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT8160.16682169908718275No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8150.16661726073045827No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8120.16600394566028479No Hit
CTTCATCTGCCGTTCCGGCCAACCACGGGGCGCACCTCTCTTTACGCGGT8030.16416400044976437No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT8020.16395956209303988No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG7790.15925747988837666No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7720.15782641139130524No Hit
CCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTTGA7660.1565997812509583No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCG7620.15578202782406034No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7600.15537315111061137No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7580.1549642743971624No Hit
TATTTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGT7470.15271545247319301No Hit
ATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCT7400.1512843839761216No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATA7330.14985331547905018No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7240.1480133702685298No Hit
CAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTG7230.14780893191180527No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT7220.1476044935550808No Hit
GTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA7030.1437201647773155No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTAACAGGCAATTTCCG6900.14106246613989717No Hit
GTCAACGAATTGTGGGTCTTTTGGGCTTCGCTGCCCCTTTTACACAATGT6890.1408580277831727No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG6870.1404491510697237No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6830.13963139764282573No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6760.13820032914575434No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6690.13676926064868292No Hit
TAGTTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGT6580.13452043872471353No Hit
CTCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAG6560.13411156201126456No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6540.1337026852978156No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTC6540.1337026852978156No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6530.13349824694109108No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6480.13247605515746863No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC6360.1300227948767748No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA6360.1300227948767748No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6340.12961391816332582No Hit
ACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC6290.12859172637970337No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA6270.12818284966625437No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6220.12716065788263192No Hit
GCCAATTACATATCCCATGAAGTTAAGGGAGTAGCCCCAACGTTTGGTTT6210.12695621952590744No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6210.12695621952590744No Hit
GCAGACGAAGAAGGGGACGGTAGAGGCCCAAACGGCCCCGAGACGGGTCG6200.12675178116918295No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATATT6200.12675178116918295No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC6160.12593402774228502No Hit
GTCTATTTACAGGCAATTTCCGAAAACAATGCTTGATTTTTTGTACAATA6120.12511627431538705No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA6100.12470739760193808No Hit
GGCCTATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG5970.12204969896451974No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5950.12164082225107076No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGGGTTTG5840.11939200032710137No Hit
CGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCT5790.11836980854347892No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5740.1173476167598565No Hit
GTTAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT5720.11693874004640752No Hit
GAATACAAGTGCAGTTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA5720.11693874004640752No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5720.11693874004640752No Hit
ATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGC5710.11673430168968302No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA5620.11489435647916262No Hit
ACATAGAGGTTCCTTGAGCAGGAATCGTGCAGGTCTTGCATGGTCCCGTG5600.11448547976571363No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5530.11305441126864223No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC5530.11305441126864223No Hit
GCTTAGTTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5500.11244109619846876No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT5450.11141890441484631No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5390.11019227427449937No Hit
GTAATTGGCAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAAAT5370.10978339756105039No Hit
GTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGTAAGAGCTTGGG5370.10978339756105039No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG5340.10917008249087695No Hit
ATATTAACATTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC5270.10773901399380552No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5240.10712569892363205No Hit
ATTAACAGGCAATTTCCGAAAACAATGCTTGATTTTTTGTACAATATGTT5240.10712569892363205No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5240.10712569892363205No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5230.10692126056690757No Hit
GGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGGT5230.10692126056690757No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5210.10651238385345858No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5210.10651238385345858No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA5200.10630794549673409No Hit
GTAACCCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGAC5200.10630794549673409No Hit
GTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAG5180.10589906878328512No Hit
GCTACTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG5170.10569463042656062No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC5170.10569463042656062No Hit
CATGTATACAAACTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5160.10549019206983615No Hit
GCTTATTTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACAT5160.10549019206983615No Hit
GCCTATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGGC5140.10508131535638718No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5130.10487687699966268No Hit
GGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCATGCGACGTGCAGAGG5110.10446800028621371No Hit
GCTATAGGCCATCGGCGCCTGAGTGGAACCTTTGTGGCTCCTCTACCGAT5100.10426356192948921No Hit
GTAGAGGAGCCACAAAGGTTCCACTCAGGCGCCGATGGCCTATAGCCAAG5060.10344580850259126No Hit
CTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCTCCA5050.10324137014586676No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCGAGCAGCC5040.10303693178914228No Hit
GTGGTAAAGTACCCCAACTGCCAATTACATATCCCATGAAGTTAAGGGAG5000.10221917836224431No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC4930.1007881098651729No Hit
CAAACTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTG4900.10017479479499944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)