FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488755

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488755
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences489145
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATCTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAG22720.4644839464780382No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17640.36062926126199796No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG16830.34406975436731435No Hit
GTGCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA14570.29786668574757996No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14110.28846252133825345No Hit
TCCCATGGCTGCTCGGGTGTGCTGCCAACTGGATCCTGCGCGGGACGTCC12470.2549346308354373No Hit
ATATTTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAG11760.24041950750799865No Hit
GTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAA11260.2301975896717742No Hit
CCCTTAACTTCATGGGATATGTAATTGGCAGTTGGGGTACTTTACCACAG11200.22897095953142726No Hit
CTCCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAACAGTCTTT11110.2271310143209069No Hit
GGCCCATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG10600.21670465812795797No Hit
GTCTATTAACAGGCAATTTCCGAAAACAATGCTTGATTTTTTGTACAATA10470.21404695949053962No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATCTCCTCCCC10260.20975375399932536No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC9620.19666969916895807No Hit
ATATGTTCCTGTGGTAAAGTACCCCAACTGCCAATTACATATCCCATGAA9500.1942164388882642No Hit
CTTCATCTGCCGTTCCGGCCAACCACGGGGCGCACCTCTCTTTACGCGGT9490.19401200053153972No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC9470.19360312381809075No Hit
CTATAGGCCATCGGCGCCTGAGTGGAACCTTTGTGGCTCCTCTACCGATC9420.1925809320344683No Hit
CTCCTAGTACAAAGACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAG9250.18910547997015198No Hit
CCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG8800.17990575391755No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8740.17867912377720307No Hit
GTAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT8670.17724805528013166No Hit
GTGTAAAAGGGGCAGCGAAGCCCAAAAGACCCACAATTCTTTGACATACT8620.1762258634965092No Hit
TACCCTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8410.17193265800529495No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG8400.17172821964857046No Hit
GTATGGATCGGTAGAGGAGCCACAAAGGTTCCACTCAGGCGCCGATGGCC8380.1713193429351215No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAG8310.16988827443805007No Hit
GGGATACCGCGTAAAGAGAGGTGCGCCCCGTGGTTGGCCGGAACGGCAGA8280.1692749593678766No Hit
GTCAACGAATTGTGGGTCTTTTGGGCTTCGCTGCCCCTTTTACACAATGT8220.16804832922752966No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8120.16600394566028479No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCAGG8080.16518619223338682No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8030.16416400044976437No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG7850.1604841100287236No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAACTGC7820.1598707949585501No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG7580.1549642743971624No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT7510.15353320590009098No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG7470.15271545247319301No Hit
TATTTGCACCTTTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGT7440.15210213740301956No Hit
ATACAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCT7280.14883112369542773No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA7230.14780893191180527No Hit
GCCATGGGAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGTTGTCAGTC7170.14658230177145837No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT7000.14310684970714205No Hit
GTCTTTAAACAAACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7000.14310684970714205No Hit
CTATATAAGAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATA6990.14290241135041756No Hit
CAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTG6940.14188021956679514No Hit
CAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGACCGCCGTGTGGTG6900.14106246613989717No Hit
ATTCTTTGACATACTTTCCAATCAATAGGTCTATTAACAGGCAATTTCCG6890.1408580277831727No Hit
GTAGAGGCCCAAACGGCCCCGAGACGGGTCGTCCGCGGGATTCAGCGCCG6370.13022723323349927No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6360.1300227948767748No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG6350.1298183565200503No Hit
ATATAAGAGAGAAACTACACGCAGCGCTTCATTCTGTGGGTCACCATATT6350.1298183565200503No Hit
CTTATATAGAATGCCAGCCTTCCACAGAGTATGTAAATAATGCCTAGTTT6340.12961391816332582No Hit
CTTGTATTCCCATCCCATCATCCTGGGCTTTCGCAAGATTCCTATGGGAG6320.12920504144987682No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA6300.12879616473642785No Hit
CATATAAAGGCATCAAGGCAGGATAGCCACATTGTGTAAAAGGGGCAGCG6200.12675178116918295No Hit
CGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTGCACTTCGCT6170.1261384660990095No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA6140.12552515102883605No Hit
GTGCAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGA6090.1245029592452136No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6070.12409408253176461No Hit
GTTAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCTTTT6030.12327632910486665No Hit
CTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA5950.12164082225107076No Hit
ATTTACATACTCTGTGGAAGGCTGGCATTCTATATAAGAGAGAAACTACA5950.12164082225107076No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTTTACC5930.12123194553762177No Hit
ACCAGGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5910.12082306882417279No Hit
GTCTATTTACAGGCAATTTCCGAAAACAATGCTTGATTTTTTGTACAATA5890.12041419211072382No Hit
CTTTTACACAATGTGGCTATCCTGCCTTGATGCCTTTATATGCATGTATA5880.12020975375399932No Hit
GTACAAAACCTTCGGACGGAAACTGCACTTGTATTCCCATCCCATCATCC5840.11939200032710137No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC5830.11918756197037687No Hit
GTTCAAATGTATACCCAAAGACAAAAGAAAATTGGTAATAGAGGTAAAAA5760.11775649347330547No Hit
GTAATTGGCAGTTGGGGTACTTTACCACAGGAACATATTGTACAAAAAAT5720.11693874004640752No Hit
GCAGACGAAGAAGGGGACGGTAGAGGCCCAAACGGCCCCGAGACGGGTCG5680.11612098661950955No Hit
TGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTG5670.11591654826278507No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5550.11346328798209121No Hit
CTATTAATAATGTCCTCCTGTAAATGAATGTTAGGAAAGGAGGGGGTTTG5530.11305441126864223No Hit
CTGCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGT5520.11284997291191774No Hit
TAGTTTGTATACATGCATATAAAGGCATCAAGGCAGGATAGCCACATTGT5510.11264553455519324No Hit
CCCATAGGAATCTTGCGAAAGCCCAGGATGATGGGATGGGAATACAAGTG5480.11203221948501978No Hit
GTAACCCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGAC5480.11203221948501978No Hit
GTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTTTTGTC5450.11141890441484631No Hit
CTCCTAGTACAAAGATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAG5390.11019227427449937No Hit
GTCCAAGAGTCCTCTTATGTAAGAGCTTGGGCAAGACCTGGTGGGCGTTC5360.10957895920432592No Hit
AGTCTGTACAACATCTTGAGTCCCTTTTTACCTCTATTACCAATTTTCTT5320.10876120577742797No Hit
CTCTAAGGCCTCCCGATACAGAGCAGAGGCGGTGTCGAGGAGATCTCGAA5260.10753457563708103No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5240.10712569892363205No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTG5210.10651238385345858No Hit
GGCCTATAGCCAAGCCCCAGCCAGTGGGGGTTGCGTCAGCAAACACTTGG5200.10630794549673409No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5200.10630794549673409No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCAC5190.1061035071400096No Hit
GACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACACCCGAGCAGCC5150.10528575371311166No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG5080.10385468521604023No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5070.10365024685931574No Hit
TTATTAATAGATGTCAACAATATGTGGGCCCTCTTACAATTAATGAAAAA4980.10181030164879534No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4940.1009925482218974No Hit
GATCCATACTGCGGAACTCCTAGCAGCTTGTTTTGCTCGCAGCCGGTCTG4930.1007881098651729No Hit
GCTACTCTGTGTTGGGGTGAGTTGATGAATCTGGCCACCTGGGTGGGAAG4920.10058367150844842No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA4900.10017479479499944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)