FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488756

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488756
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505096
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27120.536927633558769No Hit
GGATAATAGGGTTTAATTCCTTTATCTAAAGGCAAGTATTTGGTAACATT17370.34389502193642396No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT16240.32152303720480857No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC14460.2862822116983702No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGCTGAAAGTCCAA13540.2680678524478515No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT13190.26113847664602374No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT12920.25579295817032804No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACACCCGTG12370.2449039390531701No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA11760.2328270269414131No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC11680.23124316961528105No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACATAAGA11350.2247097581449863No Hit
ATTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG11090.2195622218350571No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA10750.21283082819899585No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG10480.20748530972330015No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT10230.20253575557913744No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA10090.19976400525840632No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAA9950.19699225493767522No Hit
ATCTAAAGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA9000.17818394918985697No Hit
ACCAAATACTTGCCTTTAGATAAAGGAATTAAACCCTATTATCCAGAGCA8910.17640210969795841No Hit
CTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTA8800.17422430587452684No Hit
ATGTAAGACCTTGGGCAAGTTCCGTTGGGCGTTCACGGTGGTTTCCATGC8550.16927475173036413No Hit
GGTTCAGATACTGTGTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA8540.16907676956459763No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGGGTAAAA8200.16234537592853635No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA8090.16016757210510477No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8070.15977160777357174No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8010.1585837147789727No Hit
GTCGTTGACAGTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG7890.1562079287897746No Hit
TTATTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAAC7860.15561398229247508No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTGTACCTAAC7790.15422810713210955No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT7740.153238196303277No Hit
GTTCAGATACTGTGTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7560.14967451731947987No Hit
GATATGATGTATATTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATA7510.14868460649064733No Hit
GCTTACACCTATCAATAATGTCCTCCTTCAAATGAATGTCAGGAAAAGAA7340.14531890967261668No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTG7330.14512092750685018No Hit
GCTTTATTCTTCTTCTGTACCTAACTTCAATCCTGATTGGCAAACTCCTT7270.14393303451225115No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC7270.14393303451225115No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAAGCCATGCAGTGGAA7230.1431411058491851No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA7210.14274514151765208No Hit
ATATGCAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7110.140765319859987No Hit
GGACATTATTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATG7030.13918146253385494No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTA6900.13660769437889034No Hit
GCCTAGCAGCCATGGATATGATGTATATTTGCGGGAGAGCACGACAGAAT6870.13601374788159085No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGC6740.13343997972662625No Hit
GCTTTAGGGCATGGACATCGACCCGTATAAAGAATTTGGAGCTTCTGTGG6650.13165814023472766No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC6650.13165814023472766No Hit
CCTATCAATAATGTCCTCCTTCAAATGAATGTCAGGAAAAGAAGGAGTTT6630.13126217590319464No Hit
CAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTTTG6600.13066822940589512No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6470.12809446125093052No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT6420.12710455042209798No Hit
TATTAACATTGACATAGCCGACTACTAATTCCCTGGATGCAGGATCTTCC6360.1259166574274989No Hit
CCTTTAGATAAAGGAATTAAACCCTATTATCCAGAGCATGTAGTTAATCA6350.12571867526173242No Hit
GATGCAGAGGAAGATGATAAAACGCCGCAGACACATCCAGCGATAACCAG6320.1251247287644329No Hit
CCTCTAAGGGACACTCATCCTCAAGCCATGCAGTGGAACTCCACCACTTT6310.12492674659866639No Hit
CAGTTATATGGATGATGTGGTTTTGGGGTCCAAGTCTGTACAACATCTTG6300.12472876443289989No Hit
TTCTTCTTCTGTACCTAACTTCAATCCTGATTGGCAAACTCCTTCTTTTC6250.12373885360406735No Hit
GCTCTGGATAATAGGGTTTAATTCCTTTATCTAAAGGCAAGTATTTGGTA6200.1227489427752348No Hit
ACTACATGCTCTGGATAATAGGGTTTAATTCCTTTATCTAAAGGCAAGTA6020.11918526379143767No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG5970.11819535296260512No Hit
ATACCAATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAG5880.11641351347070655No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCT5860.11601754913917355No Hit
TCCTAATACAAAGACCTTTAACCTCATCTCCTCCCCCAACTCCTCCCACT5800.1148296561445745No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG5730.11344378098420896No Hit
TCCTCAAGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG5660.11205790582384338No Hit
CTGTTAGGGGTCCCACAAATTGCTTACACCTATCAATAATGTCCTCCTTC5660.11205790582384338No Hit
ATAATGTCCTCCTTCAAATGAATGTCAGGAAAAGAAGGAGTTTGCCAATC5650.11185992365807687No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG5580.11047404849771132No Hit
CCTCAATGTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGC5570.11027606633194481No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5520.10928615550311227No Hit
CCTGTAAACAGGCCCATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5460.10809826250851323No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC5400.10691036951391418No Hit
CTTCAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGAAGG5370.10631642301661468No Hit
CTTCTGTACCTAACTTCAATCCTGATTGGCAAACTCCTTCTTTTCCTGAC5370.10631642301661468No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5330.10552449435354865No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC5320.10532651218778212No Hit
ATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACAT5300.1049305478562491No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAGGCTTTTACTTT5270.10433660135894958No Hit
CCTTTATCTAAAGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG5250.10394063702741659No Hit
GTTATATGGATGATGTGGTTTTGGGGTCCAAGTCTGTACAACATCTTGAG5250.10394063702741659No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT5240.10374265486165007No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATTGGTAT5210.10314870836435053No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGACGAGAAGGCACAG5200.10295072619858404No Hit
ACATAAGACAGGAAATGTGAAACCACAATAGTTGTCTGAGTTTTAGGCCC5130.10156485103821847No Hit
GTCTTTGGGTATACATTTAAACCCTCACAAAACAAAAAGATGGGGATATT5090.10077292237515244No Hit
ACATAGAATAGCTTGCCTGAGAGCCGTATGGTGAGGTGTACAATGTTCCG5080.10057494020938593No Hit
CTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACCTTT5070.10037695804361944No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCTCTCAGGCA5060.10017897587785293No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)