FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488757

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488757
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences505096
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN77041.5252546050651759No Hit
GGATAATAGGGTTTAATTCCTTTATCTAAAGGCAAGTATTTGGTAACATT17370.34389502193642396No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT15380.30449657094888893No Hit
CTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT12290.24332008172703803No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11790.23342097343871265No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGGACACCCGTG11460.22688756196841792No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAACATAAGA10950.21679047151432598No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG10840.21461266769089443No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACAGTGCTGAAAGTCCAA10650.21085100654133077No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT10360.20510952373410202No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA9920.19639830844037567No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT9310.18432139632861871No Hit
ATTCTATGTTGGGGTGAGTTGATGAATCTAGCCACCTGGGTGGGAAGTAA9060.17937184218445604No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA8720.17264044854839478No Hit
CTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCTTCTGGACTA8520.1686808052330646No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC8390.1661070370781No Hit
GTTTACAGGAAGTTTCCTAAAACATAATTTGATTTTTTGTACAATATGTT8340.16511712624926747No Hit
ATCTAAAGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA8080.15996958993933827No Hit
ATTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAG7860.15561398229247508No Hit
ATATGTTCCTGTGGCAAGGAGCCCCAACTCCCAATTACATATCCCATGAA7730.15304021413751048No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAATTTTAATC7520.14888258865641382No Hit
ATGTTAATATGGGCCTAAAACTCAGACAACTATTGTGGTTTCACATTTCC7340.14531890967261668No Hit
ACCAAATACTTGCCTTTAGATAAAGGAATTAAACCCTATTATCCAGAGCA7250.14353707018071812No Hit
TTATTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAAC7190.14234917718611906No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGGGTAAAA7150.14155724852305304No Hit
GGAACATATTGTACAAAAAATCAAATTATGTTTTAGGAAACTTCCTGTAA7110.140765319859987No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7050.139577426865388No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6990.13838953387078892No Hit
CCCTAACAGTAAATGAAAACAGGAGATTAAAATTAATTATGCCTGCTAGA6930.13720164087618986No Hit
GGTTCAGATACTGTGTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA6860.13581576571582432No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6730.13324199756085972No Hit
GCTTTATTCTTCTTCTGTACCTAACTTCAATCCTGATTGGCAAACTCCTT6710.1328460332293267No Hit
GCTTACACCTATCAATAATGTCCTCCTTCAAATGAATGTCAGGAAAAGAA6460.12789647908516402No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAAGCCATGCAGTGGAA6370.12611463959326544No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTGTACCTAAC6270.12413481793560036No Hit
GGACATTATTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATG6120.12116508544910275No Hit
GTTCAGATACTGTGTACGTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6100.12076912111756972No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTTCTG6090.12057113895180321No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGG6080.12037315678603672No Hit
CCTGTAAACAGGCCCATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6000.11878929945990464No Hit
ATGTAAGACCTTGGGCAAGTTCCGTTGGGCGTTCACGGTGGTTTCCATGC5960.11799737079683861No Hit
CCTATCAATAATGTCCTCCTTCAAATGAATGTCAGGAAAAGAAGGAGTTT5850.11581956697340703No Hit
CCTCTAAGGGACACTCATCCTCAAGCCATGCAGTGGAACTCCACCACTTT5820.11522562047610752No Hit
GCTCTGGATAATAGGGTTTAATTCCTTTATCTAAAGGCAAGTATTTGGTA5790.11463167397880798No Hit
CCTTTAGATAAAGGAATTAAACCCTATTATCCAGAGCATGTAGTTAATCA5690.1126518523211429No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5640.11166194149231037No Hit
GATATGATGTATATTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATA5600.11087001282924436No Hit
GCTTTAGGGCATGGACATCGACCCGTATAAAGAATTTGGAGCTTCTGTGG5560.1100780841661783No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACGGCTCTCAGGCA5560.1100780841661783No Hit
TTCTTCTTCTGTACCTAACTTCAATCCTGATTGGCAAACTCCTTCTTTTC5550.10988010200041182No Hit
GCCTAGCAGCCATGGATATGATGTATATTTGCGGGAGAGCACGACAGAAT5540.10968211983464529No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAAACAGGAGATTA5520.10928615550311227No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC5480.10849422684004624No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG5430.1075043160112137No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG5410.10710835167968069No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGATGA5370.10631642301661468No Hit
GAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCT5340.10572247651931513No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT5330.10552449435354865No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT5320.10532651218778212No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5200.10295072619858404No Hit
GTCGTTGACAGTGCTGAAAGTCCAAGAGTCCTCTTATGTAAGACCTTGGG5200.10295072619858404No Hit
CCTCTATGTTTCCCTCATGTTGCTGTACAAAACCTACGGACGGAAACTGC5190.10275274403281753No Hit
ATATGCAGCAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5180.10255476186705101No Hit
TCCTCAAGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG5070.10037695804361944No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)