FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488758

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488758
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590467
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27490.4655636978865881No Hit
CATATAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCAT22980.3891834768073406No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA17260.2923110012921975No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA15480.2621653707997229No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT14160.23981018414238223No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC14140.23947146919302856No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCAT13940.2360843196994921No Hit
GTGCTGGTGGACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13930.23591496222481528No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG13100.22185829182663894No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11870.20102732244138963No Hit
GGTTTATATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA9890.16749454245537854No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC9840.16664775508199442No Hit
CTTTTGTATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9210.15597823417735454No Hit
CGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACGTTTCCT8670.14683293054480606No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA8420.14259899367788548No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT8410.14242963620320864No Hit
CCTCCAGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8390.142090921253855No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG8220.13921184418434898No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT8140.13785698438693442No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8010.1356553372161357No Hit
GTATAAAGGGACTCAAGGTGTTGTACAGACTTGGCCCCCAAAACCACATC7870.13328433257066016No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATATAA7760.13142140034921512No Hit
TTCAAGAACAGTGTCTCTCCCAAACATAAGACAGGAAACGTGAAACCACA7680.1300665405518005No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCACATGCAGTGGAACTC7680.1300665405518005No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7590.1285423232797091No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTCCCAAACATAAGA7480.12667939105826406No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7370.12481645883681898No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7260.12295352661537394No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7230.12244545419134345No Hit
GTATTATCCAGAGGATGTAGTTAATCATTACTTCCAGACGCGACATTATT7200.12193738176731299No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG7170.12142930934328253No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7090.12007444954586793No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7080.11990509207119111No Hit
CTACATCCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT6980.11821151732442287No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6910.11702601500168512No Hit
CCTCTAAGGGACACTCATCCTCACATGCAGTGGAACTCCACCACTTTCCA6880.11651794257765463No Hit
CCCTTACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGG6880.11651794257765463No Hit
TATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6870.1163485851029778No Hit
GCCTAGCAGCCATGGAAAGGAGGTATACTTGCGGGAGAGCACGACAGAAT6730.1139775804575023No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6690.11330015055879497No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT6670.11296143560944134No Hit
GTTTACAGGAAGTTTCCTAAAGCACATTTTGATTTTGTGTACAATATGTT6640.11245336318541088No Hit
TGCTGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6600.11177593328670357No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATCCTCTGGAT6530.11059043096396581No Hit
CCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6530.11059043096396581No Hit
CTCTTTGGAAGGCGGGGATCTTATACAAAAGAGAGTCCACACGTAGCGCC6510.11025171601461216No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6500.11008235853993535No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6390.10821942631849028No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6380.10805006884381345No Hit
GCTCAGAATACTGTCTCTGCAATATCGTCAATCTCATCGAAGACTGGGGA6360.10771135389445981No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6360.10771135389445981No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA6320.10703392399575253No Hit
GTATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA6210.10517099177430746No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6130.10381613197689286No Hit
GGATATCCACATTGTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6110.10347741702753921No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA6090.10313870207818558No Hit
ATATAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCATG6080.10296934460350875No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA6070.10279998712883193No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6050.10246127217947829No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTTTCAACAATGTCCTCCTGC6010.10178384228077099No Hit
GCATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCTTGAGG6010.10178384228077099No Hit
GTTGAAAGATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAG5990.10144512733141733No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)