FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488759

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488759
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences590467
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN27430.4645475530385271No Hit
CATATAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCAT21880.37055415459289004No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA16440.27842368836869796No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA14630.24776998545219292No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG14110.2389633967689981No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13890.23523753232610797No Hit
GTGCTGGTGGACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC13860.2347294599020775No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC13410.22710837354162047No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCAT13330.22575351374420588No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT12120.20526125930831018No Hit
ATATTAACGTTGACATAGCCGACTACTAATTCCCTGGATGCTGGATCTTC9950.1685106873034395No Hit
CTTTTGTATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9530.16139767336701288No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTGAAA9290.15733309397476913No Hit
GGTTTATATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA9090.15394594448123264No Hit
CCTCCAGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8810.14920393519028158No Hit
GAAACCAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT8580.14530871327271463No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATATAA8500.14395385347530004No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCACATGCAGTGGAACTC8370.14175220630450136No Hit
CGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACGTTTCCT8330.14107477640579405No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG8200.13887312923499534No Hit
GTATTATCCAGAGGATGTAGTTAATCATTACTTCCAGACGCGACATTATT8130.13768762691225758No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7910.13396176246936745No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTGTCTCTCCCAAACATAAGA7900.13379240499469064No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7600.12871168075438594No Hit
GTATAAAGGGACTCAAGGTGTTGTACAGACTTGGCCCCCAAAACCACATC7560.12803425085567863No Hit
TTCAAGAACAGTGTCTCTCCCAAACATAAGACAGGAAACGTGAAACCACA7510.1271874634822945No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG7450.1261713186342336No Hit
GTGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT7430.12583260368487995No Hit
CCTCTAAGGGACACTCATCCTCACATGCAGTGGAACTCCACCACTTTCCA7260.12295352661537394No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC7210.12210673924198982No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA7210.12210673924198982No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA7160.12125995186860569No Hit
CCCTTACAGTAAATGAAACCAGGAGACTAAAATTAATTATGCCTGCTAGG7120.12058252196989841No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTATG7030.119058304697807No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7010.11871958974845334No Hit
AGTCTGTACAACACCTTGAGTCCCTTTATACCGCTGTTACCAATTTTCTT6890.11668730005233147No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6830.11567115520427051No Hit
GTATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGTCTGGA6700.1134695080334718No Hit
CTCTTTGGAAGGCGGGGATCTTATACAAAAGAGAGTCCACACGTAGCGCC6680.11313079308411816No Hit
GCTTTATTCTTCTACGGTACCTTGCTTTAATCCTAATTGGCAAACTCCTT6660.11279207813476451No Hit
GTTTACAGGAAGTTTCCTAAAGCACATTTTGATTTTGTGTACAATATGTT6650.11262272066008769No Hit
CTTCAAAGACTGTGTGTTTACTGAGTGGGAGGAGTTGGGGGAGGAGGTTA6610.11194529076138039No Hit
ATATAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTCACTTCATG6490.10991300106525852No Hit
CTACATCCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATAT6480.1097436435905817No Hit
CCTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6450.10923557116655122No Hit
TCTTTCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6400.1083887837931671No Hit
GTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACATCCTCTGGAT6320.10703392399575253No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG6230.10550970672366111No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6230.10550970672366111No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6180.104662919350277No Hit
GCCTAGCAGCCATGGAAAGGAGGTATACTTGCGGGAGAGCACGACAGAAT6140.1039854894515697No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6120.10364677450221604No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6070.10279998712883193No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6020.1019531997554478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)