FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488760

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488760
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences544693
Sequences flagged as poor quality0
Sequence length35-151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAATAGGGTTTGATTCCTTTATCCAAGGGCAAGTATTTGGTAACATT19460.35726546880536375No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGAATGTAAATAATGTCTCGT19260.3535936757035615No Hit
CATCTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT14740.2706111516028295No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAACACC13400.2460101378207541No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13300.24417424126985293No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT12970.23811578265187913No Hit
ATTGATAGGTGTGAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAG12400.2276511723117426No Hit
ATATAAGACCTTGGTCAGGTTCCAGTGGGCGTTCACGGTGGTCTCCATGC12210.2241629688650304No Hit
ATATAAGATACCCGCCTTCCAAAGAGAATGTAAATAATGTCTCGTCTGGA11980.21994040679795776No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT11830.21718656197160605No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGTAACACCCGTG11300.20745631025182992No Hit
GTCTTTGAAGTATGTCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA10830.19882759646259454No Hit
CTCCAAAAGACACCAAATATTCAAGAACAACTTCTCTTCCAAAAGTAAGA10060.18469119302065568No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT9720.17844914474759177No Hit
ATATGTTCCTGTGGCAGGGAACCCCAACTCCCAATTACATATCCCATGAA9480.174042993025429No Hit
ATGTTAATATGGGCCTAAAATTAAGACAACTATTGTGGTTTCACATTTCC9050.16614863785655407No Hit
GTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAATTAACTTTAGTC8980.16486351027092325No Hit
CCTATCAATAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT8760.16082453785894074No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA8650.15880505165294947No Hit
CCCTAACAGTAAATGAAATCAGGAGACTAAAGTTAATTATGCCTGCTAGA8630.15843787234276924No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGTGTAAAA8620.1582542826876791No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8590.15770351372240876No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGGCCCCCATG8570.15733633441222855No Hit
GCTTTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTT8500.15605120682659773No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG8420.1545824895858768No Hit
ACCAAATACTTGCCCTTGGATAAAGGAATCAAACCCTATTATCCCGAGCA8340.15311377234515589No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG8240.15127787579425475No Hit
GTCTTATATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT8180.15017633786371404No Hit
CTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGGTACCTAAC8070.1481568516577228No Hit
CCTTTATCCAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGG7970.14632095510682164No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG7910.14521941717628095No Hit
GTTCTGTACAGGGTCCCCAGTCTTCGAGAAGGTTGACGATATGGCAGAGG7900.14503582752119082No Hit
CTTTTACACAATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATA7830.14375069993556003No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7690.14118044476429842No Hit
ATCCAAGGGCAAGTATTTGGTAACATTGGGATAAAATCTAGCAGGCATAA7600.1395281378684874No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA7540.1384265999379467No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAGACGGGCAAC7380.13548916545650486No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT7230.13273532063015314No Hit
ACATTATTGATAGGTGTGAGCAATTTGTGGGACCCCTAACAGTAAATGAA7220.132551730975063No Hit
TCCAAAGAGAATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA7200.13218455166488277No Hit
ATTTAATTTTTTGTACAATATGTTCCTGTGGCAGGGAACCCCAACTCCCA7190.1320009620097927No Hit
GTGTTGTCTCTCTTTTATATAAGATACCCGCCTTCCAAAGAGAATGTAAA7000.1285127585630805No Hit
GGGTAAAGGTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGA6850.12575891373672876No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAAC6850.12575891373672876No Hit
GTATCGGGAGGCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACGG6850.12575891373672876No Hit
GTTCAGATACTGTTTACTTAGAAAGGCCTTGTAAGTTGGCGAGAAAGTAA6660.12227071029001657No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTACTATTCG6620.12153635166965611No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6540.1200676344289352No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6440.11823173787803404No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGGAAAACCCC6430.11804814822294393No Hit
GGTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6430.11804814822294393No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCC6410.11768096891276371No Hit
GGATAAAGGAATCAAACCCTATTATCCCGAGCATGTAGTTAATCATTACT6290.11547789305168232No Hit
AATTTGAGGACAACAAGTTGGTGAGTGACTGGAGATTTGGGACTGCGAAT6290.11547789305168232No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC6290.11547789305168232No Hit
GTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCA6220.11419276546605153No Hit
CAGTAAATGAAATCAGGAGACTAAAGTTAATTATGCCTGCTAGATTTTAT6190.11364199650078118No Hit
GAATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG6180.11345840684569106No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6170.11327481719060094No Hit
GTTTAGATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6150.11290763788042071No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGATAGCACGACAGAAT6040.11088815167442945No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCTAAACAG5980.10978661374388876No Hit
CTCTTATATAAGACCTTGGTCAGGTTCCAGTGGGCGTTCACGGTGGTCTC5940.10905225512352829No Hit
TGATAGGTGTGAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAGGA5910.10850148615825796No Hit
AGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTGTTACCAATTTTCTT5910.10850148615825796No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5880.10795071719298761No Hit
TTCCCCCACTGTCTGGCTTTCAGTTACATGGATGATGTGGTATTGGGGGC5830.10703276891753702No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5810.10666558960735681No Hit
GATTAACTACATGCTCGGGATAATAGGGTTTGATTCCTTTATCCAAGGGC5790.10629841029717657No Hit
CTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCT5780.10611482064208647No Hit
ATACCACATCATCCATGTAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5730.1051968723666359No Hit
GGGAATTAGTAGTCGGCTATGTCAATGTTAATATGGGCCTAAAATTAAGA5730.1051968723666359No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5700.10464610340136554No Hit
GTACAGAACATGGAGAACATCTCATCAGGACTCCTAGGACCCCTGCTCGT5660.10391174478100508No Hit
GTTAATATGGGCCTAAAATTAAGACAACTATTGTGGTTTCACATTTCCTG5600.1028102068504644No Hit
ATCAAGGTATGTTGCCCGTCTGTCCTCTAATTCCAGGATCATCAACCACC5590.10262661719537428No Hit
ACCTAACTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATT5590.10262661719537428No Hit
CCCAATTACATATCCCATGAAATTAAGGGAATATCCCCATCTTTTTGTTT5580.10244302754028416No Hit
TATTAACATTGACATAGCCGACTACTAATTCCCTGGATGCTGGGTCTTCC5530.10152507926483359No Hit
CTGTTAGGGGTCCCACAAATTGCTCACACCTATCAATAATGTCTTCCTGC5530.10152507926483359No Hit
GTAAACACACAGTCTTTGAAGTATGTCTCAAGGTCGGTCGTTGACATTGC5510.10115789995465337No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)