FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488762

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488762
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences803057
Sequences flagged as poor quality0
Sequence length35-151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN143091.7818162347131028No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATATGTTCAGG21290.2651119409954711No Hit
CATTTAAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTCAT17510.21804180774216525No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAACCTTAT17430.21704561444579898No Hit
CTCTTACAGTTAACGAAAAAAGGAGATTAAAATTGATCATGCCTGCTAGG17040.2121891721260135No Hit
GTGTAAAGGGAGCGGCAAAACCTAAAAGACCCACAATTCTTTGACATACT16560.2062120123478159No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA13420.1671114254654402No Hit
ATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAATATGTT12590.15677592001564022No Hit
GGTTAGGATAGAACCTAGCAGGCATGATCAATTTTAATCTCCTTTTTTCG11650.14507064878333667No Hit
ATATGTTCCTGTGGCAAGGTACCCCAACTTCCAATTACATATCCCATGAA11100.13822181987081864No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAAAGAATTGTGGGTCT10870.13535776414376563No Hit
GGATAATAAGGTTTGATGCCTTTATCTAAGGGCAAATATTTGGTAAGGTT10690.13311632922694155No Hit
GGATATTATCAATAGATGTCAACAATATGTGGGCCCTCTTACAGTTAACG10640.13249370841671265No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTAGGAAACTTCCTATAA10000.12452416204578255No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT9990.12439963788373677No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTGCCACAG9510.1184224781055392No Hit
CTGTAAGAGGGCCCACATATTGTTGACATCTATTGATAATATCCTCTTTC8970.11169817335506696No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGATAAAGGCATCAAAC8710.10846054514187661No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG8630.10746435184551034No Hit
GGTCTATTTATAGGAAGTTTCCTAAAACATTGTTTGATTTTTTGTACAAT8380.10435124779436579No Hit
GGGTAAAGGTTCAAATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA8130.10123814374322121No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)