FastQCFastQC Report
Sun 4 Sep 2022
EGAF00002488765

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488765
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences810025
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN127461.573531681120953No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT32390.399864201722169No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA28700.354310052158884No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC26860.3315947038671646No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGAACAAAGACC22190.273942162278942No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGAAACAGTGAGCCCTGCTCAGA19670.24283201135767415No Hit
GGATAATAAGGTTTGATCCCTTTATCTAAGGGCAAATATTTTGTAACATT19420.23974568686151665No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT18250.22530168821949942No Hit
TTATTATCCAGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT15990.19740131477423536No Hit
CTCCAAAAGACACCAAATATTCCAGAACAGTTTGTCTCCCAAAAGTAAGA15950.19690750285485015No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC15710.19394463133853893No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG15440.1906114008826888No Hit
GACTCTGGGATCTTGAAGAGTTTGGTGGAAAGTGGTGGAGTTCCACTGCA14730.18184623931360142No Hit
GGAATATGATAAAACGCCGCAGACACATCCAGCGATAGCCAGGACAAATT14720.18172278633375513No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG14170.17493287244220856No Hit
CAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCGTTTTGTTTTG13980.17258726582512887No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGTCCACACGTAGCGCC13690.16900712940958612No Hit
CATTTAAACCCTCACAAAACAAAACGATGGGGATATTCCCTTAACTTCAT13290.16406901021573408No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGTGGGTCACCATATT13000.16048887380019136No Hit
ACATCGACCCGTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTT12970.16011851486065243No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACACCCGTG12720.15703219036449492No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAG12680.15653837844510973No Hit
ATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCA12390.15295824202956698No Hit
CTCTTTTATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTC12240.15110644733187248No Hit
CCCAAAGTCAGGGCCCTGTACTTTCCTGCTGGTGGCTCCAGTTCAGAAAC12020.14839048177525385No Hit
GTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGGAT11950.14752631091632973No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG11670.14406962748063332No Hit
ATATAAGATACCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGTCTGGA11650.14382272152094072No Hit
GTTTTATCCCAATGTTACAAAATATTTGCCCTTAGATAAAGGGATCAAAC11550.1425881917224777No Hit
TAGATAAAGGGATCAAACCTTATTATCCAGAGCATGTAGTTAATCATTAC11540.14246473874263138No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGACAGTCCAA11390.1406129440449369No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCCA11220.13851424338754975No Hit
ATCATCTTCCTCTGCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT10660.1316008765161569No Hit
TCCATCATCCAGGGAATTAGTAGTCGGTTATGTCAACGTTAATATGGGCC10530.129995987778155No Hit
CGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCT10240.12641585136261227No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAACATTCG10140.12518132156414927No Hit
GGTGAAGATAAGGGAGTTGGCTGCCTTCCTGACTGCCGATTGGTGGAGGC9990.12332952686645474No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC9730.1201197493904509No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG9730.1201197493904509No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC9710.11987284343075832No Hit
TTCCAGAACAGTTTGTCTCCCAAAAGTAAGACAGGAAATGTGAAACCACA9620.1187617666121416No Hit
GATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAGTCTTCGATAAGAT9610.1186383136322953No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA9310.11493472423690627No Hit
GCCTTAGAGTCTCCGGAACATTGTACACCTCACCATACAGCAATCAGGCA9130.11271257059967284No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG8790.10851516928489863No Hit
GTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCTGG8790.10851516928489863No Hit
GAATCAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAAC8780.10839171630505232No Hit
GGAAAGTACAGGGCCCTGACTTTGGGATCTTGAAGAGTTTGGTGGAAAGT8750.10802135736551341No Hit
TTTTAAGTTTTTGTACAATATGTTCCTGCGGCAATGTGCCCCAACTCCCA8400.10370050307089287No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC8390.10357705009104658No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8120.10024381963519645No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)