FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002488766

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002488766
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477858
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN22380.46833996710319803No Hit
ACTCACAACTGTGCCAGCAGCACCTCCTCCTGCCTCCACCAATCGGCAGT13550.2835570399574769No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA13380.27999949775874844No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC11880.24860941953467347No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11650.24379627420698197No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC11370.23793679293848802No Hit
GTTGTGAGTATGCCCTGAGCCTGAGGGCTCCACCCCAACAGACCCCCATG10660.22307882257909256No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTTCCAAAAGTAAGA9100.1904331412260546No Hit
ATGTAAGACCTTGGGCAGGTTCCAGTGGGCGTTCACGGTGGTCTCCATGC8240.17243616304425163No Hit
GTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCGTTGACATACT8170.17097129272712813No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTAATATTCG8070.16887862084552313No Hit
CCCTTATCTCCACCTCTAAGGGAAACTCATCCTCAGGCCATGCAGTGGAA7810.16343767395335015No Hit
CTATTATCCCGAGCATGTAGTTAATCATTACTTCCAGACGAGACATTATT7800.16322840676518965No Hit
CTTTTATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGT7710.16134500207174515No Hit
GCCTAACAGCCATGGAAACGATGTATACTTGCGGGATAGCACGACAGAAT7680.16071720050726365No Hit
ATATAAAAGAGAGACAACACGTAGCGCCTCATTTTGCGGGTCACCATATT7610.15925233019014018No Hit
TCCAAAGAGTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA7110.14878897078211517No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAATTTCAT7080.1481611692176337No Hit
ATGTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCC6930.1450221613952262No Hit
GTATCGGGAGGCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACGG6840.1431387567017817No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA6750.1412553520083372No Hit
CTTATAAGGCCTTTCTAAGTAAACAATATCAGAACCTTTACCCCGTTGCT6570.13748854262144822No Hit
CTATTCTTCTACGGTACCTAACTTTAATCCTGATTGGCAAACTCCTTCTT6510.13623293949248522No Hit
GTCCGGTGCTGGTGGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6300.1318383285411147No Hit
GTATGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACATGCTCGGG6130.12828078634238624No Hit
CCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6120.12807151915422574No Hit
GGATAATAGGGTTTGATTCCTTTATCTAAGGGGAAGTATTTGGTAACATT6070.12702518321342324No Hit
CATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTATAAGGCC6060.12681591602526274No Hit
CTCTTTGGAAGGCGGGTATCTTATATAAAAGAGAGACAACACGTAGCGCC5970.12493251133181825No Hit
GCTTACACCTATCAACAATGTCTTCCTGCAAATGAATGTCAGGAAAAGAA5960.12472324414365774No Hit
GGGTAAAGGTTCTGATATTGTTTACTTAGAAAGGCCTTATAAGTTGGCGA5940.12430470976733674No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG5920.12388617539101573No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5900.12346764101469475No Hit
GCACCGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG5890.12325837382653425No Hit
GTATGGATGGGCAGAGGAGACACAAAGGTTCCGCGCATGCGCTGATGGCC5820.12179350350941073No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5820.12179350350941073No Hit
ATGTATACTTGCGGGATAGCACGACAGAATTGTCAGTCCCGATAAGTTTT5770.12074716756860825No Hit
GTACAATATGTTCCTGTGGCAAGGAACCCCAACTCCCAATTACATATCCC5750.12032863319228725No Hit
ATATGTTCCTGTGGCAAGGAACCCCAACTCCCAATTACATATCCCATGAA5740.12011936600412675No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC5740.12011936600412675No Hit
CTGTTAGGGGTCCCACAAATTGCTTACACCTATCAACAATGTCTTCCTGC5730.11991009881596625No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5680.11886376287516376No Hit
ATTCTGAGCAGGGCTCACTGTCCCTGAACTGGAGCCACCAGCAGGAAAGT5640.11802669412252174No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACCACC5620.11760815974620076No Hit
GTTGATAGGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAG5600.11718962536987976No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG5520.11551548786459577No Hit
GCCTTAGAGTCCCCGGAACATTGTTCACCTCACCATACGGCAATCAGGCA5510.11530622067643527No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG5440.11384135035931175No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC5430.11363208317115127No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGACCGAGATCTTCTGCGAC5410.11321354879483027No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAACTCCAGATTGGGACCT5410.11321354879483027No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCATCAAACTCTTCAAG5340.11174867847770678No Hit
ATACCACATCATCCATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTA5300.11091160972506477No Hit
ATCCAGCATCCAGGGAATTAGTAGTCGGCTATGTCAATGTTAATATGGGC5300.11091160972506477No Hit
GGTGTAAGCAATTTGTGGGACCCCTAACAGTAAATGAAATCAGAAGACTA5290.11070234253690427No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTATGCCAAGTGTTTGCTG5290.11070234253690427No Hit
GTATTCATGTATACAAGCTAAACAGGCTTTTACTTTCTCGCCAACTTATA5280.11049307534874377No Hit
GTCTTACATAAGAGGACTCTTGGACTTTCAGCAATGTCAACGACCGACCT5270.11028380816058327No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGATTCCCGAGACCGAGATCT5210.10902820503162028No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTTCT5140.10756333471449678No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC5090.10651699877369428No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5080.10630773158553378No Hit
ATATAAGATACCCGCCTTCCAAAGAGTATGTAAATAATGTCTCGTCTGGA5060.10588919720921279No Hit
CTTTAATCCTGATTGGCAAACTCCTTCTTTTCCTGACATTCATTTGCAGG5050.10567993002105229No Hit
TATCCACATTGTGTAAAAGGGGCAGCGAACCCCAGAAGACCCACAATTCG5040.10547066283289179No Hit
TCCCGATACAGAGCAGAGGCGGTGTCGAGAAGATCTCGAATATTAGGAAA5030.10526139564473128No Hit
GCTCAGAATACTGCCTCTGCCATATCGTCAACCTTCTCGAAGACTGGGGA4930.10316872376312629No Hit
CTTTTACACAATGTGGATATCCTGCCTTACTGCCTTTGTATTCATGTATA4930.10316872376312629No Hit
GTTAATATGGGCCTAAAATTCAGACAACTATTGTGGTTTCACATTTCCTG4930.10316872376312629No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAACGGCACTAGTAAACTGAG4910.10275018938680529No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGTGGATTGGGGACAGAAAGAT4890.10233165501048427No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)